Consensus recommendations for trabecular meshwork cell isolation, characterization and culture

Kate E. Keller, Sanjoy K. Bhattacharya, Theresa Borrás, Thomas M. Brunner, Sunee Chansangpetch, Abbott F. Clark, W. Michael Dismuke, Yiqin Du, Michael H. Elliott, C. Ross Ethier, Jennifer A. Faralli, Thomas F. Freddo, Rudolf Fuchshofer, Michael Giovingo, Haiyan Gong, Pedro Gonzalez, Alex Huang, Murray A. Johnstone, Paul L. Kaufman, Mary J. Kelley & 37 others Paul A. Knepper, Casey C. Kopczynski, John G. Kuchtey, Rachel W. Kuchtey, Markus H. Kuehn, Raquel L. Lieberman, Shan C. Lin, Paloma Liton, Yutao Liu, Elke Lütjen-Drecoll, Weiming Mao, Marisse Masis-Solano, Fiona McDonnell, Colleen M. McDowell, Darryl R. Overby, Padmanabhan P. Pattabiraman, Vijay K. Raghunathan, P. Vasanth Rao, Douglas J. Rhee, Uttio Roy Chowdhury, Paul Russell, John R. Samples, Donald Schwartz, Evan B. Stubbs, Ernst R. Tamm, James C. Tan, Carol B. Toris, Karen Y. Torrejon, Janice A. Vranka, Mary K. Wirtz, Thomas Yorio, Jie Zhang, Gulab S. Zode, Michael P. Fautsch, Donna M. Peters, Ted S. Acott, W. Daniel Stamer

Research output: Contribution to journalShort survey

9 Citations (Scopus)

Abstract

Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

Original languageEnglish (US)
Pages (from-to)164-173
Number of pages10
JournalExperimental eye research
Volume171
DOIs
StatePublished - Jun 1 2018

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Trabecular Meshwork
Cell Separation
Cell Culture Techniques
Ocular Hypertension
Intraocular Pressure
Research Personnel

Keywords

  • Aqueous humor dynamics
  • Conventional outflow
  • Glaucoma
  • Intraocular pressure
  • Ocular hypertension

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Keller, K. E., Bhattacharya, S. K., Borrás, T., Brunner, T. M., Chansangpetch, S., Clark, A. F., ... Stamer, W. D. (2018). Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Experimental eye research, 171, 164-173. https://doi.org/10.1016/j.exer.2018.03.001

Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. / Keller, Kate E.; Bhattacharya, Sanjoy K.; Borrás, Theresa; Brunner, Thomas M.; Chansangpetch, Sunee; Clark, Abbott F.; Dismuke, W. Michael; Du, Yiqin; Elliott, Michael H.; Ethier, C. Ross; Faralli, Jennifer A.; Freddo, Thomas F.; Fuchshofer, Rudolf; Giovingo, Michael; Gong, Haiyan; Gonzalez, Pedro; Huang, Alex; Johnstone, Murray A.; Kaufman, Paul L.; Kelley, Mary J.; Knepper, Paul A.; Kopczynski, Casey C.; Kuchtey, John G.; Kuchtey, Rachel W.; Kuehn, Markus H.; Lieberman, Raquel L.; Lin, Shan C.; Liton, Paloma; Liu, Yutao; Lütjen-Drecoll, Elke; Mao, Weiming; Masis-Solano, Marisse; McDonnell, Fiona; McDowell, Colleen M.; Overby, Darryl R.; Pattabiraman, Padmanabhan P.; Raghunathan, Vijay K.; Rao, P. Vasanth; Rhee, Douglas J.; Chowdhury, Uttio Roy; Russell, Paul; Samples, John R.; Schwartz, Donald; Stubbs, Evan B.; Tamm, Ernst R.; Tan, James C.; Toris, Carol B.; Torrejon, Karen Y.; Vranka, Janice A.; Wirtz, Mary K.; Yorio, Thomas; Zhang, Jie; Zode, Gulab S.; Fautsch, Michael P.; Peters, Donna M.; Acott, Ted S.; Stamer, W. Daniel.

In: Experimental eye research, Vol. 171, 01.06.2018, p. 164-173.

Research output: Contribution to journalShort survey

Keller, KE, Bhattacharya, SK, Borrás, T, Brunner, TM, Chansangpetch, S, Clark, AF, Dismuke, WM, Du, Y, Elliott, MH, Ethier, CR, Faralli, JA, Freddo, TF, Fuchshofer, R, Giovingo, M, Gong, H, Gonzalez, P, Huang, A, Johnstone, MA, Kaufman, PL, Kelley, MJ, Knepper, PA, Kopczynski, CC, Kuchtey, JG, Kuchtey, RW, Kuehn, MH, Lieberman, RL, Lin, SC, Liton, P, Liu, Y, Lütjen-Drecoll, E, Mao, W, Masis-Solano, M, McDonnell, F, McDowell, CM, Overby, DR, Pattabiraman, PP, Raghunathan, VK, Rao, PV, Rhee, DJ, Chowdhury, UR, Russell, P, Samples, JR, Schwartz, D, Stubbs, EB, Tamm, ER, Tan, JC, Toris, CB, Torrejon, KY, Vranka, JA, Wirtz, MK, Yorio, T, Zhang, J, Zode, GS, Fautsch, MP, Peters, DM, Acott, TS & Stamer, WD 2018, 'Consensus recommendations for trabecular meshwork cell isolation, characterization and culture', Experimental eye research, vol. 171, pp. 164-173. https://doi.org/10.1016/j.exer.2018.03.001
Keller KE, Bhattacharya SK, Borrás T, Brunner TM, Chansangpetch S, Clark AF et al. Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. Experimental eye research. 2018 Jun 1;171:164-173. https://doi.org/10.1016/j.exer.2018.03.001
Keller, Kate E. ; Bhattacharya, Sanjoy K. ; Borrás, Theresa ; Brunner, Thomas M. ; Chansangpetch, Sunee ; Clark, Abbott F. ; Dismuke, W. Michael ; Du, Yiqin ; Elliott, Michael H. ; Ethier, C. Ross ; Faralli, Jennifer A. ; Freddo, Thomas F. ; Fuchshofer, Rudolf ; Giovingo, Michael ; Gong, Haiyan ; Gonzalez, Pedro ; Huang, Alex ; Johnstone, Murray A. ; Kaufman, Paul L. ; Kelley, Mary J. ; Knepper, Paul A. ; Kopczynski, Casey C. ; Kuchtey, John G. ; Kuchtey, Rachel W. ; Kuehn, Markus H. ; Lieberman, Raquel L. ; Lin, Shan C. ; Liton, Paloma ; Liu, Yutao ; Lütjen-Drecoll, Elke ; Mao, Weiming ; Masis-Solano, Marisse ; McDonnell, Fiona ; McDowell, Colleen M. ; Overby, Darryl R. ; Pattabiraman, Padmanabhan P. ; Raghunathan, Vijay K. ; Rao, P. Vasanth ; Rhee, Douglas J. ; Chowdhury, Uttio Roy ; Russell, Paul ; Samples, John R. ; Schwartz, Donald ; Stubbs, Evan B. ; Tamm, Ernst R. ; Tan, James C. ; Toris, Carol B. ; Torrejon, Karen Y. ; Vranka, Janice A. ; Wirtz, Mary K. ; Yorio, Thomas ; Zhang, Jie ; Zode, Gulab S. ; Fautsch, Michael P. ; Peters, Donna M. ; Acott, Ted S. ; Stamer, W. Daniel. / Consensus recommendations for trabecular meshwork cell isolation, characterization and culture. In: Experimental eye research. 2018 ; Vol. 171. pp. 164-173.
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abstract = "Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.",
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AU - Keller, Kate E.

AU - Bhattacharya, Sanjoy K.

AU - Borrás, Theresa

AU - Brunner, Thomas M.

AU - Chansangpetch, Sunee

AU - Clark, Abbott F.

AU - Dismuke, W. Michael

AU - Du, Yiqin

AU - Elliott, Michael H.

AU - Ethier, C. Ross

AU - Faralli, Jennifer A.

AU - Freddo, Thomas F.

AU - Fuchshofer, Rudolf

AU - Giovingo, Michael

AU - Gong, Haiyan

AU - Gonzalez, Pedro

AU - Huang, Alex

AU - Johnstone, Murray A.

AU - Kaufman, Paul L.

AU - Kelley, Mary J.

AU - Knepper, Paul A.

AU - Kopczynski, Casey C.

AU - Kuchtey, John G.

AU - Kuchtey, Rachel W.

AU - Kuehn, Markus H.

AU - Lieberman, Raquel L.

AU - Lin, Shan C.

AU - Liton, Paloma

AU - Liu, Yutao

AU - Lütjen-Drecoll, Elke

AU - Mao, Weiming

AU - Masis-Solano, Marisse

AU - McDonnell, Fiona

AU - McDowell, Colleen M.

AU - Overby, Darryl R.

AU - Pattabiraman, Padmanabhan P.

AU - Raghunathan, Vijay K.

AU - Rao, P. Vasanth

AU - Rhee, Douglas J.

AU - Chowdhury, Uttio Roy

AU - Russell, Paul

AU - Samples, John R.

AU - Schwartz, Donald

AU - Stubbs, Evan B.

AU - Tamm, Ernst R.

AU - Tan, James C.

AU - Toris, Carol B.

AU - Torrejon, Karen Y.

AU - Vranka, Janice A.

AU - Wirtz, Mary K.

AU - Yorio, Thomas

AU - Zhang, Jie

AU - Zode, Gulab S.

AU - Fautsch, Michael P.

AU - Peters, Donna M.

AU - Acott, Ted S.

AU - Stamer, W. Daniel

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N2 - Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

AB - Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.

KW - Aqueous humor dynamics

KW - Conventional outflow

KW - Glaucoma

KW - Intraocular pressure

KW - Ocular hypertension

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DO - 10.1016/j.exer.2018.03.001

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JO - Experimental Eye Research

JF - Experimental Eye Research

SN - 0014-4835

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