Conservation of Amino Acid Sequences Between Human and Porcine Choline Acetyltransferase

Louis B. Hersh, Karen Takane, Karen Gylys, Carolyn Moomaw, Clive A. Slaughter

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Abstract: The amino acid sequence of 11 peptides generated from human placental choline acetyltransferase was compared to the corresponding amino acid sequences predicted from the nucleotide sequence of a recently cloned porcine choline acetyltransferase cDNA. These peptides, which were generated by cyanogen bromide cleavage or tryptic digestion, accounted for 23% of the amino acids in the enzyme. Of the 145 amino acids sequenced eight differed between the two species, yielding an identity of 94% over the regions sampled. Of the eight amino acids that differed six could represent single base changes in the DNA sequence. These findings demonstrate strong sequence similarity between porcine and human choline acetyltransferase and indicate that they are closely related evolutionarily.

Original languageEnglish (US)
Pages (from-to)1843-1845
Number of pages3
JournalJournal of Neurochemistry
Volume51
Issue number6
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

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Keywords

  • Amino acid sequences
  • Choline acetyltransferase
  • Homology

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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