Conservation of the egg-laying hormone neuropeptide and attractin pheromone in the spotted sea hare, Aplysia dactylomela

Scott F. Cummins, Parinyaporn Nuurai, Gregg Thomas Nagle, Bernard M. Degnan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In the marine opisthobranch mollusc, Aplysia, secreted peptides and proteins play an essential role in egg laying and mate attraction. Aplysia californica egg laying is initiated by secretion of the egg-laying hormone (ELH) peptide while mate attraction is made possible by protein pheromones, such as attractin, released into the surrounding seawater with the egg cordon. In this study, we investigated the existence of similar egg-laying hormone and attractin products in the spotted sea hare, Aplysia dactylomela, a species that is widely distributed in almost all tropical and temperate oceans, including Australia's Great Barrier Reef. Immunological analysis revealed that an ELH-like transmitter is present within bag cell somata and processes of the abdominal ganglion. A molecular genetic approach found that the ELH precursor mRNA is synthesized in the abdominal ganglia and encodes a 36-residue peptide (dELH) that is cleaved from the prohormone prior to secretion. It is most closely related to A. californica and A. brasiliana ELH (91.7% identical). We also found that A. dactylomela synthesize an attractin pheromone in the albumen gland that is released during egg laying. The gene encodes a 58-residue mature protein that is 74.9% similar to A. californica attractin. We demonstrate that an increase in seawater temperature can disrupt attractins higher order interactions, such as those with the pheromone temptin, and accelerates attractin degradation. Together, these findings further expands our understanding of pheromone intermolecular interactions and presents an opportunity for further study of how increases in sea water temperature may affect this important marine communication system.

Original languageEnglish (US)
Pages (from-to)394-401
Number of pages8
JournalPeptides
Volume31
Issue number3
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

Fingerprint

Hares
Aplysia
Pheromones
Neuropeptides
Oceans and Seas
Ovum
Conservation
Hormones
Seawater
Molluscs
Peptides
Proteins
Reefs
Peptide Hormones
RNA Precursors
Carisoprodol
Ganglia
Transmitters
Communication systems
Genes

Keywords

  • Aplysia
  • Attractin
  • Egg-laying hormone
  • Pheromone
  • Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Conservation of the egg-laying hormone neuropeptide and attractin pheromone in the spotted sea hare, Aplysia dactylomela. / Cummins, Scott F.; Nuurai, Parinyaporn; Nagle, Gregg Thomas; Degnan, Bernard M.

In: Peptides, Vol. 31, No. 3, 01.03.2010, p. 394-401.

Research output: Contribution to journalArticle

Cummins, Scott F. ; Nuurai, Parinyaporn ; Nagle, Gregg Thomas ; Degnan, Bernard M. / Conservation of the egg-laying hormone neuropeptide and attractin pheromone in the spotted sea hare, Aplysia dactylomela. In: Peptides. 2010 ; Vol. 31, No. 3. pp. 394-401.
@article{d8c6a6e0185a4867b346ae318eaa8bf6,
title = "Conservation of the egg-laying hormone neuropeptide and attractin pheromone in the spotted sea hare, Aplysia dactylomela",
abstract = "In the marine opisthobranch mollusc, Aplysia, secreted peptides and proteins play an essential role in egg laying and mate attraction. Aplysia californica egg laying is initiated by secretion of the egg-laying hormone (ELH) peptide while mate attraction is made possible by protein pheromones, such as attractin, released into the surrounding seawater with the egg cordon. In this study, we investigated the existence of similar egg-laying hormone and attractin products in the spotted sea hare, Aplysia dactylomela, a species that is widely distributed in almost all tropical and temperate oceans, including Australia's Great Barrier Reef. Immunological analysis revealed that an ELH-like transmitter is present within bag cell somata and processes of the abdominal ganglion. A molecular genetic approach found that the ELH precursor mRNA is synthesized in the abdominal ganglia and encodes a 36-residue peptide (dELH) that is cleaved from the prohormone prior to secretion. It is most closely related to A. californica and A. brasiliana ELH (91.7{\%} identical). We also found that A. dactylomela synthesize an attractin pheromone in the albumen gland that is released during egg laying. The gene encodes a 58-residue mature protein that is 74.9{\%} similar to A. californica attractin. We demonstrate that an increase in seawater temperature can disrupt attractins higher order interactions, such as those with the pheromone temptin, and accelerates attractin degradation. Together, these findings further expands our understanding of pheromone intermolecular interactions and presents an opportunity for further study of how increases in sea water temperature may affect this important marine communication system.",
keywords = "Aplysia, Attractin, Egg-laying hormone, Pheromone, Temperature",
author = "Cummins, {Scott F.} and Parinyaporn Nuurai and Nagle, {Gregg Thomas} and Degnan, {Bernard M.}",
year = "2010",
month = "3",
day = "1",
doi = "10.1016/j.peptides.2009.10.010",
language = "English (US)",
volume = "31",
pages = "394--401",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Conservation of the egg-laying hormone neuropeptide and attractin pheromone in the spotted sea hare, Aplysia dactylomela

AU - Cummins, Scott F.

AU - Nuurai, Parinyaporn

AU - Nagle, Gregg Thomas

AU - Degnan, Bernard M.

PY - 2010/3/1

Y1 - 2010/3/1

N2 - In the marine opisthobranch mollusc, Aplysia, secreted peptides and proteins play an essential role in egg laying and mate attraction. Aplysia californica egg laying is initiated by secretion of the egg-laying hormone (ELH) peptide while mate attraction is made possible by protein pheromones, such as attractin, released into the surrounding seawater with the egg cordon. In this study, we investigated the existence of similar egg-laying hormone and attractin products in the spotted sea hare, Aplysia dactylomela, a species that is widely distributed in almost all tropical and temperate oceans, including Australia's Great Barrier Reef. Immunological analysis revealed that an ELH-like transmitter is present within bag cell somata and processes of the abdominal ganglion. A molecular genetic approach found that the ELH precursor mRNA is synthesized in the abdominal ganglia and encodes a 36-residue peptide (dELH) that is cleaved from the prohormone prior to secretion. It is most closely related to A. californica and A. brasiliana ELH (91.7% identical). We also found that A. dactylomela synthesize an attractin pheromone in the albumen gland that is released during egg laying. The gene encodes a 58-residue mature protein that is 74.9% similar to A. californica attractin. We demonstrate that an increase in seawater temperature can disrupt attractins higher order interactions, such as those with the pheromone temptin, and accelerates attractin degradation. Together, these findings further expands our understanding of pheromone intermolecular interactions and presents an opportunity for further study of how increases in sea water temperature may affect this important marine communication system.

AB - In the marine opisthobranch mollusc, Aplysia, secreted peptides and proteins play an essential role in egg laying and mate attraction. Aplysia californica egg laying is initiated by secretion of the egg-laying hormone (ELH) peptide while mate attraction is made possible by protein pheromones, such as attractin, released into the surrounding seawater with the egg cordon. In this study, we investigated the existence of similar egg-laying hormone and attractin products in the spotted sea hare, Aplysia dactylomela, a species that is widely distributed in almost all tropical and temperate oceans, including Australia's Great Barrier Reef. Immunological analysis revealed that an ELH-like transmitter is present within bag cell somata and processes of the abdominal ganglion. A molecular genetic approach found that the ELH precursor mRNA is synthesized in the abdominal ganglia and encodes a 36-residue peptide (dELH) that is cleaved from the prohormone prior to secretion. It is most closely related to A. californica and A. brasiliana ELH (91.7% identical). We also found that A. dactylomela synthesize an attractin pheromone in the albumen gland that is released during egg laying. The gene encodes a 58-residue mature protein that is 74.9% similar to A. californica attractin. We demonstrate that an increase in seawater temperature can disrupt attractins higher order interactions, such as those with the pheromone temptin, and accelerates attractin degradation. Together, these findings further expands our understanding of pheromone intermolecular interactions and presents an opportunity for further study of how increases in sea water temperature may affect this important marine communication system.

KW - Aplysia

KW - Attractin

KW - Egg-laying hormone

KW - Pheromone

KW - Temperature

UR - http://www.scopus.com/inward/record.url?scp=77049114260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77049114260&partnerID=8YFLogxK

U2 - 10.1016/j.peptides.2009.10.010

DO - 10.1016/j.peptides.2009.10.010

M3 - Article

VL - 31

SP - 394

EP - 401

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 3

ER -