Consistent chromosomal anomalies in keratinocyte cell lines derived from untreated malignant lesions of the oral cavity

Vyomesh Patel, W. Andrew Yeudall, Anne Gardner, Serdar Mutlu, Crispian Scully, Stephen S. Prime

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22 Scopus citations

Abstract

Cytogenetic analysis has been carried out on 8 early passage cell lines derived from 8 untreated human oral squamous cell carcinomas. Clonal aberrations were detected in the karyotypes of each cell line. A high frequency of breakpoints were noted on chromosomes 1, 7, 8, 9, 11, and X. An isochromosome 8 was present in 6 out of 8 cell lines; isochromosome 9 (3 cell lines) and isochromosome 11 (1 cell line) were also found. In 4 out of 8 cell lines the X chromosome harboured breakpoints, a novel finding in oral squamous cell carcinomas. Breakpoints were common on chromosome 1, with 1p12–p13 most frequently involved. Tandem duplication of 11q13–q23, which contains a number of growth regulatory genes, was also noted in 2 cases. We correlate the sites of proto‐oncogenes and other growth control genes with chromosomal breakpoints and suggest that several of these may play a role in the pathogenesis of oral cancer. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalGenes, Chromosomes and Cancer
Volume7
Issue number2
DOIs
Publication statusPublished - Jun 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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