Consistent chromosomal anomalies in keratinocyte cell lines derived from untreated malignant lesions of the oral cavity

Vyomesh Patel, William Andrew Yeudall, Anne Gardner, Serdar Mutlu, Crispian Scully, Stephen S. Prime

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Cytogenetic analysis has been carried out on 8 early passage cell lines derived from 8 untreated human oral squamous cell carcinomas. Clonal aberrations were detected in the karyotypes of each cell line. A high frequency of breakpoints were noted on chromosomes 1, 7, 8, 9, 11, and X. An isochromosome 8 was present in 6 out of 8 cell lines; isochromosome 9 (3 cell lines) and isochromosome 11 (1 cell line) were also found. In 4 out of 8 cell lines the X chromosome harboured breakpoints, a novel finding in oral squamous cell carcinomas. Breakpoints were common on chromosome 1, with 1p12–p13 most frequently involved. Tandem duplication of 11q13–q23, which contains a number of growth regulatory genes, was also noted in 2 cases. We correlate the sites of proto‐oncogenes and other growth control genes with chromosomal breakpoints and suggest that several of these may play a role in the pathogenesis of oral cancer. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalGenes, Chromosomes and Cancer
Volume7
Issue number2
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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Keratinocytes
Mouth
Isochromosomes
Cell Line
Chromosomes, Human, Pair 1
Squamous Cell Carcinoma
Chromosome Breakpoints
Chromosomes, Human, Pair 7
Cytogenetic Analysis
Mouth Neoplasms
X Chromosome
Regulator Genes
Growth
Karyotype
Genes

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

Cite this

Consistent chromosomal anomalies in keratinocyte cell lines derived from untreated malignant lesions of the oral cavity. / Patel, Vyomesh; Yeudall, William Andrew; Gardner, Anne; Mutlu, Serdar; Scully, Crispian; Prime, Stephen S.

In: Genes, Chromosomes and Cancer, Vol. 7, No. 2, 01.01.1993, p. 109-115.

Research output: Contribution to journalArticle

Patel, Vyomesh ; Yeudall, William Andrew ; Gardner, Anne ; Mutlu, Serdar ; Scully, Crispian ; Prime, Stephen S. / Consistent chromosomal anomalies in keratinocyte cell lines derived from untreated malignant lesions of the oral cavity. In: Genes, Chromosomes and Cancer. 1993 ; Vol. 7, No. 2. pp. 109-115.
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