Contemporary Methacrylate Resin-based Root Canal Sealers Exhibit Different Degrees of Ex Vivo Cytotoxicity When Cured in Their Self-cured Mode

Jason M. Ames, Robert J. Loushine, Brian R. Babb, Thomas E. Bryan, Petra E. Lockwood, Mai Sui, Steven Roberts, R. Norman Weller, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The cytotoxicity of four methacrylate resin-based sealers was investigated by the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay, which measures cell viability by assessing its succinate dehydrogenase activity. The sealers were polymerized in the self-cured mode to simulate the setting condition upon their extrusion into periradicular tissues. Disks were prepared from EndoREZ (Ultradent, South Jordan, UT), RealSeal (SybronEndo, Orange, CA), MetaSEAL (Parkell, Farmington, NY), and RealSeal SE (SybronEndo) together with positive and negative controls. After setting, they were placed in direct contact with rat osteosarcoma (ROS 17/2.8) cells and for 5 succeeding weeks after immersing in simulated body fluid (SBF). All sealers exhibited severe toxicity initially (week 0). EndoREZ and RealSeal remained severely toxic after five cycles of SBF immersion. Toxicity of the two self-etching resin-based sealers MetaSEAL and RealSeal SE decreased gradually over time. Transmission electron microscopy of cells exposed to RealSeal SE showed variable degrees of cell injury that reflect its toxicity status. Cells with intact mitochondria were identifiable after the sealer became noncytotoxic at week 5.

Original languageEnglish (US)
Pages (from-to)225-228
Number of pages4
JournalJournal of Endodontics
Volume35
Issue number2
DOIs
StatePublished - Feb 1 2009

Fingerprint

Methacrylates
Dental Pulp Cavity
Body Fluids
Jordan
Succinate Dehydrogenase
Poisons
Immersion
Osteosarcoma
Transmission Electron Microscopy
Bromides
Cell Survival
Mitochondria
canals sealer
Wounds and Injuries

Keywords

  • Cytotoxicity
  • MTT assay
  • methacrylate resin-based sealers
  • nonetching
  • self-adhesive
  • self-etching primer
  • succinate dehydrogenase

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Contemporary Methacrylate Resin-based Root Canal Sealers Exhibit Different Degrees of Ex Vivo Cytotoxicity When Cured in Their Self-cured Mode. / Ames, Jason M.; Loushine, Robert J.; Babb, Brian R.; Bryan, Thomas E.; Lockwood, Petra E.; Sui, Mai; Roberts, Steven; Weller, R. Norman; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Journal of Endodontics, Vol. 35, No. 2, 01.02.2009, p. 225-228.

Research output: Contribution to journalArticle

Ames, Jason M. ; Loushine, Robert J. ; Babb, Brian R. ; Bryan, Thomas E. ; Lockwood, Petra E. ; Sui, Mai ; Roberts, Steven ; Weller, R. Norman ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Contemporary Methacrylate Resin-based Root Canal Sealers Exhibit Different Degrees of Ex Vivo Cytotoxicity When Cured in Their Self-cured Mode. In: Journal of Endodontics. 2009 ; Vol. 35, No. 2. pp. 225-228.
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