Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization

Q. Song, K. Jiao, L. Tonggu, L. G. Wang, S. L. Zhang, Y. D. Yang, L. Zhang, J. H. Bian, D. X. Hao, C. Y. Wang, Y. X. Ma, D. D. Arola, L. Breschi, J. H. Chen, Franklin Chi Meng Tay, L. N. Niu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Contemporary models of intrafibrillar mineralization mechanisms are established using collagen fibrils as templates without considering the contribution from collagen-bound apatite nucleation inhibitors. However, collagen matrices destined for mineralization in vertebrates contain bound matrix proteins for intrafibrillar mineralization. Negatively charged, high–molecular weight polycarboxylic acid is cross-linked to reconstituted collagen to create a model for examining the contribution of collagen-ligand interaction to intrafibrillar mineralization. Cryogenic electron microscopy and molecular dynamics simulation show that, after cross-linking to collagen, the bound polyelectrolyte caches prenucleation cluster singlets into chain-like aggregates along the fibrillar surface to increase the pool of mineralization precursors available for intrafibrillar mineralization. Higher-quality mineralized scaffolds with better biomechanical properties are achieved compared with mineralization of unmodified scaffolds in polyelectrolyte-stabilized mineralization solution. Collagen-ligand interaction provides insights on the genesis of heterogeneously mineralized tissues and the potential causes of ectopic calcification in nonmineralized body tissues.

Original languageEnglish (US)
Article numbereaav9075
JournalScience Advances
Volume5
Issue number3
DOIs
StatePublished - Mar 29 2019

Fingerprint

biomimetics
collagens
ligands
interactions
calcification
vertebrates
apatites
matrices
inhibitors
cryogenics
electron microscopy
templates
nucleation
molecular dynamics
proteins
acids
causes

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)
  • General

Cite this

Song, Q., Jiao, K., Tonggu, L., Wang, L. G., Zhang, S. L., Yang, Y. D., ... Niu, L. N. (2019). Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization. Science Advances, 5(3), [eaav9075]. https://doi.org/10.1126/sciadv.aav9075

Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization. / Song, Q.; Jiao, K.; Tonggu, L.; Wang, L. G.; Zhang, S. L.; Yang, Y. D.; Zhang, L.; Bian, J. H.; Hao, D. X.; Wang, C. Y.; Ma, Y. X.; Arola, D. D.; Breschi, L.; Chen, J. H.; Tay, Franklin Chi Meng; Niu, L. N.

In: Science Advances, Vol. 5, No. 3, eaav9075, 29.03.2019.

Research output: Contribution to journalArticle

Song, Q, Jiao, K, Tonggu, L, Wang, LG, Zhang, SL, Yang, YD, Zhang, L, Bian, JH, Hao, DX, Wang, CY, Ma, YX, Arola, DD, Breschi, L, Chen, JH, Tay, FCM & Niu, LN 2019, 'Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization', Science Advances, vol. 5, no. 3, eaav9075. https://doi.org/10.1126/sciadv.aav9075
Song, Q. ; Jiao, K. ; Tonggu, L. ; Wang, L. G. ; Zhang, S. L. ; Yang, Y. D. ; Zhang, L. ; Bian, J. H. ; Hao, D. X. ; Wang, C. Y. ; Ma, Y. X. ; Arola, D. D. ; Breschi, L. ; Chen, J. H. ; Tay, Franklin Chi Meng ; Niu, L. N. / Contribution of biomimetic collagen-ligand interaction to intrafibrillar mineralization. In: Science Advances. 2019 ; Vol. 5, No. 3.
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