Controversial role of epstein-barr virus in multiple sclerosis

Nazneen Fatima, Michael P. Toscano, Stephen B. Hunter, Cynthia Cohen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The role of Epstein-Barr virus (EBV) in the pathogenesis of multiple sclerosis (MS) is still elusive. In 2007, Serafini et al demonstrated the direct role of EBV in brain lesions of MS patients. They found positive immunohistochemistry (IHC) staining for latency membrane protein 1 (LMP1), and EBV-encoded RNA (EBER) by in-situ hybridization (ISH) within postmortem brains of MS patients. The goal of this study was to attempt to demonstrate LMP1 by IHC and EBER by ISH in brains of patients with MS, to either support or refute their findings. Seventeen MS (16 brain biopsies and 1 autopsy brain) and 12 autopsy brains with no pathologic abnormalities, as normal controls, were studied. To control for the possibility that inflammation owing to other etiologies could result in EBV-positive cell accumulation, 11 brain biopsies of encephalitis and 4 brain biopsies of progressive multifocal leukoencephalopathy were also studied. Known positive (Hodgkins and non-Hodgkins lymphoma) and negative (with antibody primary replaced by buffer) controls were used. All positive and negative controls showed appropriate staining. However, there were no positive LMP1 or EBER results in any of the groups studied. The negative results of IHC and ISH in our study sharply contrast to those previously mentioned by Serafini et al, 2007 and suggest that EBV is not directly related to MS as an etiology.

Original languageEnglish (US)
Pages (from-to)246-252
Number of pages7
JournalApplied Immunohistochemistry and Molecular Morphology
Volume19
Issue number3
DOIs
StatePublished - May 1 2011

Fingerprint

Human Herpesvirus 4
Multiple Sclerosis
Brain
In Situ Hybridization
Membrane Proteins
Immunohistochemistry
Biopsy
Autopsy
RNA
Staining and Labeling
Progressive Multifocal Leukoencephalopathy
Encephalitis
Hodgkin Disease
Non-Hodgkin's Lymphoma
Buffers
Inflammation
Antibodies

Keywords

  • EBV-encoded RNA
  • immunohistochemistry
  • in-situ hybridization
  • latency membrane protein
  • multiple sclerosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology
  • Histology

Cite this

Controversial role of epstein-barr virus in multiple sclerosis. / Fatima, Nazneen; Toscano, Michael P.; Hunter, Stephen B.; Cohen, Cynthia.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 19, No. 3, 01.05.2011, p. 246-252.

Research output: Contribution to journalArticle

Fatima, Nazneen ; Toscano, Michael P. ; Hunter, Stephen B. ; Cohen, Cynthia. / Controversial role of epstein-barr virus in multiple sclerosis. In: Applied Immunohistochemistry and Molecular Morphology. 2011 ; Vol. 19, No. 3. pp. 246-252.
@article{7bf0f08ad5094b55b38bc05663769b6e,
title = "Controversial role of epstein-barr virus in multiple sclerosis",
abstract = "The role of Epstein-Barr virus (EBV) in the pathogenesis of multiple sclerosis (MS) is still elusive. In 2007, Serafini et al demonstrated the direct role of EBV in brain lesions of MS patients. They found positive immunohistochemistry (IHC) staining for latency membrane protein 1 (LMP1), and EBV-encoded RNA (EBER) by in-situ hybridization (ISH) within postmortem brains of MS patients. The goal of this study was to attempt to demonstrate LMP1 by IHC and EBER by ISH in brains of patients with MS, to either support or refute their findings. Seventeen MS (16 brain biopsies and 1 autopsy brain) and 12 autopsy brains with no pathologic abnormalities, as normal controls, were studied. To control for the possibility that inflammation owing to other etiologies could result in EBV-positive cell accumulation, 11 brain biopsies of encephalitis and 4 brain biopsies of progressive multifocal leukoencephalopathy were also studied. Known positive (Hodgkins and non-Hodgkins lymphoma) and negative (with antibody primary replaced by buffer) controls were used. All positive and negative controls showed appropriate staining. However, there were no positive LMP1 or EBER results in any of the groups studied. The negative results of IHC and ISH in our study sharply contrast to those previously mentioned by Serafini et al, 2007 and suggest that EBV is not directly related to MS as an etiology.",
keywords = "EBV-encoded RNA, immunohistochemistry, in-situ hybridization, latency membrane protein, multiple sclerosis",
author = "Nazneen Fatima and Toscano, {Michael P.} and Hunter, {Stephen B.} and Cynthia Cohen",
year = "2011",
month = "5",
day = "1",
doi = "10.1097/PAI.0b013e3181fcf3b4",
language = "English (US)",
volume = "19",
pages = "246--252",
journal = "Applied Immunohistochemistry and Molecular Morphology",
issn = "1541-2016",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Controversial role of epstein-barr virus in multiple sclerosis

AU - Fatima, Nazneen

AU - Toscano, Michael P.

AU - Hunter, Stephen B.

AU - Cohen, Cynthia

PY - 2011/5/1

Y1 - 2011/5/1

N2 - The role of Epstein-Barr virus (EBV) in the pathogenesis of multiple sclerosis (MS) is still elusive. In 2007, Serafini et al demonstrated the direct role of EBV in brain lesions of MS patients. They found positive immunohistochemistry (IHC) staining for latency membrane protein 1 (LMP1), and EBV-encoded RNA (EBER) by in-situ hybridization (ISH) within postmortem brains of MS patients. The goal of this study was to attempt to demonstrate LMP1 by IHC and EBER by ISH in brains of patients with MS, to either support or refute their findings. Seventeen MS (16 brain biopsies and 1 autopsy brain) and 12 autopsy brains with no pathologic abnormalities, as normal controls, were studied. To control for the possibility that inflammation owing to other etiologies could result in EBV-positive cell accumulation, 11 brain biopsies of encephalitis and 4 brain biopsies of progressive multifocal leukoencephalopathy were also studied. Known positive (Hodgkins and non-Hodgkins lymphoma) and negative (with antibody primary replaced by buffer) controls were used. All positive and negative controls showed appropriate staining. However, there were no positive LMP1 or EBER results in any of the groups studied. The negative results of IHC and ISH in our study sharply contrast to those previously mentioned by Serafini et al, 2007 and suggest that EBV is not directly related to MS as an etiology.

AB - The role of Epstein-Barr virus (EBV) in the pathogenesis of multiple sclerosis (MS) is still elusive. In 2007, Serafini et al demonstrated the direct role of EBV in brain lesions of MS patients. They found positive immunohistochemistry (IHC) staining for latency membrane protein 1 (LMP1), and EBV-encoded RNA (EBER) by in-situ hybridization (ISH) within postmortem brains of MS patients. The goal of this study was to attempt to demonstrate LMP1 by IHC and EBER by ISH in brains of patients with MS, to either support or refute their findings. Seventeen MS (16 brain biopsies and 1 autopsy brain) and 12 autopsy brains with no pathologic abnormalities, as normal controls, were studied. To control for the possibility that inflammation owing to other etiologies could result in EBV-positive cell accumulation, 11 brain biopsies of encephalitis and 4 brain biopsies of progressive multifocal leukoencephalopathy were also studied. Known positive (Hodgkins and non-Hodgkins lymphoma) and negative (with antibody primary replaced by buffer) controls were used. All positive and negative controls showed appropriate staining. However, there were no positive LMP1 or EBER results in any of the groups studied. The negative results of IHC and ISH in our study sharply contrast to those previously mentioned by Serafini et al, 2007 and suggest that EBV is not directly related to MS as an etiology.

KW - EBV-encoded RNA

KW - immunohistochemistry

KW - in-situ hybridization

KW - latency membrane protein

KW - multiple sclerosis

UR - http://www.scopus.com/inward/record.url?scp=79955074523&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955074523&partnerID=8YFLogxK

U2 - 10.1097/PAI.0b013e3181fcf3b4

DO - 10.1097/PAI.0b013e3181fcf3b4

M3 - Article

C2 - 21494180

AN - SCOPUS:79955074523

VL - 19

SP - 246

EP - 252

JO - Applied Immunohistochemistry and Molecular Morphology

JF - Applied Immunohistochemistry and Molecular Morphology

SN - 1541-2016

IS - 3

ER -