Cooperative effect of the lipopolysaccharide and culinary-medicinal cauliflower mushroom sparassis crispa (Wulf.) Fr. (Aphyllophoromycetideae)- derived β-glucan on inflammatory cytokine secretion by the murine macrophage cell line

Sung In Kim, Hyuk Gu Park, Gye Hyung Cho, In Soo Ko, Ha Won Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

β-Glucans act as pathogen-associated molecular patterns (PAMPs), which can bind to the pattern recognition receptors (PRRs) on a macrophage or dendritic cell. Many kinds of mushroom β-glucans act as PAMPs and can bind to dectin-1 as one of the PRRs. High-molecular-weight β-glucans or intestinal normal microbiota can be transported through lumen epithelial cells, resulting in the stimulation of a macrophage in Peyer's patch. To study the involvement of TLR4 in the stimulation of dectin-1, the RAW264.7 macrophage cell line was stimulated using the β-glucan of Sparassis crispa (BGS) as well as the lipopolysaccharide (LPS) as a TLR4 ligand. The induction of IL-6 and the inducible nitric oxide synthase (iNOS) mRNAs was increased in a BGS and TNF-α concentration-dependent pattern. The increases of the TNF-α and IL-6 secretions after their treatment with 10 μg/mL BGS were found to be 204- and 420-fold, which are much higher, compared to those of the control. Moreover, the secretions of such cytokines were further increased by the presence of LPS, indicating the involvement of TLR4 in the signaling by dectin-1 with BGS. The secretion of nitric oxide (NO) was increased by BGS. These results show that BGS stimulated innate immunity by activating the macrophages through the induction of the dectin-1 receptor and the inflammatory cytokines. The secretion of inflammatory cytokines was influenced by TLR4 stimulation.

Original languageEnglish (US)
Pages (from-to)9-20
Number of pages12
JournalInternational Journal of Medicinal Mushrooms
Volume11
Issue number1
DOIs
StatePublished - Apr 24 2009
Externally publishedYes

Fingerprint

Glucans
Agaricales
Brassica
Lipopolysaccharides
Macrophages
Cytokines
Pattern Recognition Receptors
Cell Line
Interleukin-6
Peyer's Patches
Cytokine Receptors
Nitric Oxide Synthase Type II
Innate Immunity
Dendritic Cells
Nitric Oxide
Molecular Weight
Epithelial Cells
Ligands
Messenger RNA
dectin 1

Keywords

  • ?-glucan
  • Dectin-1
  • Inducible nitric oxide synthase
  • Interleukin
  • Medicinal mushrooms
  • Nitric oxide
  • Nuclear factor-kappa B
  • Sparassis crispa
  • TLR4
  • Tumor necrosis factor-?

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Pharmacology
  • Drug Discovery

Cite this

@article{ed8a8b3bd6964afe8e12dfb967b9671f,
title = "Cooperative effect of the lipopolysaccharide and culinary-medicinal cauliflower mushroom sparassis crispa (Wulf.) Fr. (Aphyllophoromycetideae)- derived β-glucan on inflammatory cytokine secretion by the murine macrophage cell line",
abstract = "β-Glucans act as pathogen-associated molecular patterns (PAMPs), which can bind to the pattern recognition receptors (PRRs) on a macrophage or dendritic cell. Many kinds of mushroom β-glucans act as PAMPs and can bind to dectin-1 as one of the PRRs. High-molecular-weight β-glucans or intestinal normal microbiota can be transported through lumen epithelial cells, resulting in the stimulation of a macrophage in Peyer's patch. To study the involvement of TLR4 in the stimulation of dectin-1, the RAW264.7 macrophage cell line was stimulated using the β-glucan of Sparassis crispa (BGS) as well as the lipopolysaccharide (LPS) as a TLR4 ligand. The induction of IL-6 and the inducible nitric oxide synthase (iNOS) mRNAs was increased in a BGS and TNF-α concentration-dependent pattern. The increases of the TNF-α and IL-6 secretions after their treatment with 10 μg/mL BGS were found to be 204- and 420-fold, which are much higher, compared to those of the control. Moreover, the secretions of such cytokines were further increased by the presence of LPS, indicating the involvement of TLR4 in the signaling by dectin-1 with BGS. The secretion of nitric oxide (NO) was increased by BGS. These results show that BGS stimulated innate immunity by activating the macrophages through the induction of the dectin-1 receptor and the inflammatory cytokines. The secretion of inflammatory cytokines was influenced by TLR4 stimulation.",
keywords = "?-glucan, Dectin-1, Inducible nitric oxide synthase, Interleukin, Medicinal mushrooms, Nitric oxide, Nuclear factor-kappa B, Sparassis crispa, TLR4, Tumor necrosis factor-?",
author = "Kim, {Sung In} and Park, {Hyuk Gu} and Cho, {Gye Hyung} and Ko, {In Soo} and Kim, {Ha Won}",
year = "2009",
month = "4",
day = "24",
doi = "10.1615/IntJMedMushr.v11.i1.20",
language = "English (US)",
volume = "11",
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T1 - Cooperative effect of the lipopolysaccharide and culinary-medicinal cauliflower mushroom sparassis crispa (Wulf.) Fr. (Aphyllophoromycetideae)- derived β-glucan on inflammatory cytokine secretion by the murine macrophage cell line

AU - Kim, Sung In

AU - Park, Hyuk Gu

AU - Cho, Gye Hyung

AU - Ko, In Soo

AU - Kim, Ha Won

PY - 2009/4/24

Y1 - 2009/4/24

N2 - β-Glucans act as pathogen-associated molecular patterns (PAMPs), which can bind to the pattern recognition receptors (PRRs) on a macrophage or dendritic cell. Many kinds of mushroom β-glucans act as PAMPs and can bind to dectin-1 as one of the PRRs. High-molecular-weight β-glucans or intestinal normal microbiota can be transported through lumen epithelial cells, resulting in the stimulation of a macrophage in Peyer's patch. To study the involvement of TLR4 in the stimulation of dectin-1, the RAW264.7 macrophage cell line was stimulated using the β-glucan of Sparassis crispa (BGS) as well as the lipopolysaccharide (LPS) as a TLR4 ligand. The induction of IL-6 and the inducible nitric oxide synthase (iNOS) mRNAs was increased in a BGS and TNF-α concentration-dependent pattern. The increases of the TNF-α and IL-6 secretions after their treatment with 10 μg/mL BGS were found to be 204- and 420-fold, which are much higher, compared to those of the control. Moreover, the secretions of such cytokines were further increased by the presence of LPS, indicating the involvement of TLR4 in the signaling by dectin-1 with BGS. The secretion of nitric oxide (NO) was increased by BGS. These results show that BGS stimulated innate immunity by activating the macrophages through the induction of the dectin-1 receptor and the inflammatory cytokines. The secretion of inflammatory cytokines was influenced by TLR4 stimulation.

AB - β-Glucans act as pathogen-associated molecular patterns (PAMPs), which can bind to the pattern recognition receptors (PRRs) on a macrophage or dendritic cell. Many kinds of mushroom β-glucans act as PAMPs and can bind to dectin-1 as one of the PRRs. High-molecular-weight β-glucans or intestinal normal microbiota can be transported through lumen epithelial cells, resulting in the stimulation of a macrophage in Peyer's patch. To study the involvement of TLR4 in the stimulation of dectin-1, the RAW264.7 macrophage cell line was stimulated using the β-glucan of Sparassis crispa (BGS) as well as the lipopolysaccharide (LPS) as a TLR4 ligand. The induction of IL-6 and the inducible nitric oxide synthase (iNOS) mRNAs was increased in a BGS and TNF-α concentration-dependent pattern. The increases of the TNF-α and IL-6 secretions after their treatment with 10 μg/mL BGS were found to be 204- and 420-fold, which are much higher, compared to those of the control. Moreover, the secretions of such cytokines were further increased by the presence of LPS, indicating the involvement of TLR4 in the signaling by dectin-1 with BGS. The secretion of nitric oxide (NO) was increased by BGS. These results show that BGS stimulated innate immunity by activating the macrophages through the induction of the dectin-1 receptor and the inflammatory cytokines. The secretion of inflammatory cytokines was influenced by TLR4 stimulation.

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KW - Interleukin

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KW - TLR4

KW - Tumor necrosis factor-?

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DO - 10.1615/IntJMedMushr.v11.i1.20

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VL - 11

SP - 9

EP - 20

JO - International Journal of Medicinal Mushrooms

JF - International Journal of Medicinal Mushrooms

SN - 1521-9437

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