Correlation between vascular and cellular responses to atrial natriuretic peptide and to sodium nitroprusside

Walter H. Newman, Li Ming Zhang, Manuel R. Castresana, Mark G. Currie, Bernhard F. Becker

Research output: Contribution to journalArticle

2 Scopus citations


Atrial natriuretic peptide (ANP) and sodium nitroprusside (SNP) increase cGMP in vascular smooth muscle cells and act as vasodilators in some, but not all, blood vessels. In this present study, we attempted to correlate the ability of these two agents to dilate blood vessels with the ability to increase cGMP in cultured vascular smooth muscle cells. In the isolated guinea pig heart, SNP dose-dependently increased coronary flow while ANP was ineffective. In smooth muscle cells cultured from the coronary system, SNP increased intracellular cGMP in a dose-dependent manner while ANP had no effect on cGMP in these cells. In isolated guinea-pig thoracic aorta, precontracted with K+, both ANP and SNP produced relaxation and ANP was the more potent. In smooth muscle cells cultured from the aorta, ANP and SNP increased cGMP and the potency relationship was similar to the intact vessel. These results support the view that phenotypic properties of vascular smooth muscle cells can account for differences in the response of blood vessels to vasodilators.

Original languageEnglish (US)
Pages (from-to)1351-1356
Number of pages6
JournalLife sciences
Issue number18
Publication statusPublished - 1994
Externally publishedYes



  • atrial natriuretic peptide
  • blood vessels
  • sodium nitroprusside
  • vascular smooth muscle cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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