Correlation of cytotoxicity, filler loading and curing time of dental composites

Wyatt Franklin Caughman, Gretchen B Caughman, Robert A. Shiflett, Frederick Rueggeberg, George S. Schuster

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

Previous studies have shown that dental resin composites tested in cell culture produce cytotoxic effects on human gingival tissues. In this study, the cytotoxic potential of resin composites on primary human gingival fibroblast cultures was evaluated, based on inhibition of cellular protein synthesis measured by [35S] methionine incorporation. Both resin content and percentage of monomer conversion were considered as potential causes of cytotoxicity. Three resin composites were selected to provide a range of filler content from 45 to 86 wt%. Duplicate sample discs (1 mm thick x 10 mm diameter) of each composite were polymerized for 15, 30 and 60 s, followed by heat (110°C, 10 min), and the degree of monomer conversion for each sample group was measured using Fourier transform infrared spectrophotometry. Identically fabricated discs were placed into 35 mm culture dishes with gingival fibroblasts and incubated for 24 h at 37°C. The cell monolayers then were labelled at 24 h with [35S] methionine, washed and solubilized; then incorporated radioactivity was quantitated by liquid scintillation spectrometry. For each composite, as the percentage of monomer conversion increased, cellular toxicity decreased. In comparing different composites having similar monomer conversions, it was found that the filler resin ratio was not the only factor determining the composite's relative toxicity.

Original languageEnglish (US)
Pages (from-to)737-740
Number of pages4
JournalBiomaterials
Volume12
Issue number8
DOIs
StatePublished - Jan 1 1991

Fingerprint

Dental composites
Composite Resins
Cytotoxicity
Methionine
Curing
Fillers
Tooth
Fibroblasts
Infrared Spectrophotometry
Monomers
Cell culture
Resins
Composite materials
Fourier Analysis
Radioactivity
Toxicity
Spectrum Analysis
Cell Culture Techniques
Hot Temperature
Spectrophotometry

Keywords

  • Cytotoxicity
  • composites
  • dental materials

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Correlation of cytotoxicity, filler loading and curing time of dental composites. / Caughman, Wyatt Franklin; Caughman, Gretchen B; Shiflett, Robert A.; Rueggeberg, Frederick; Schuster, George S.

In: Biomaterials, Vol. 12, No. 8, 01.01.1991, p. 737-740.

Research output: Contribution to journalArticle

@article{44d21e2a67c84f76b3e04b4bbf772898,
title = "Correlation of cytotoxicity, filler loading and curing time of dental composites",
abstract = "Previous studies have shown that dental resin composites tested in cell culture produce cytotoxic effects on human gingival tissues. In this study, the cytotoxic potential of resin composites on primary human gingival fibroblast cultures was evaluated, based on inhibition of cellular protein synthesis measured by [35S] methionine incorporation. Both resin content and percentage of monomer conversion were considered as potential causes of cytotoxicity. Three resin composites were selected to provide a range of filler content from 45 to 86 wt{\%}. Duplicate sample discs (1 mm thick x 10 mm diameter) of each composite were polymerized for 15, 30 and 60 s, followed by heat (110°C, 10 min), and the degree of monomer conversion for each sample group was measured using Fourier transform infrared spectrophotometry. Identically fabricated discs were placed into 35 mm culture dishes with gingival fibroblasts and incubated for 24 h at 37°C. The cell monolayers then were labelled at 24 h with [35S] methionine, washed and solubilized; then incorporated radioactivity was quantitated by liquid scintillation spectrometry. For each composite, as the percentage of monomer conversion increased, cellular toxicity decreased. In comparing different composites having similar monomer conversions, it was found that the filler resin ratio was not the only factor determining the composite's relative toxicity.",
keywords = "Cytotoxicity, composites, dental materials",
author = "Caughman, {Wyatt Franklin} and Caughman, {Gretchen B} and Shiflett, {Robert A.} and Frederick Rueggeberg and Schuster, {George S.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1016/0142-9612(91)90022-3",
language = "English (US)",
volume = "12",
pages = "737--740",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "8",

}

TY - JOUR

T1 - Correlation of cytotoxicity, filler loading and curing time of dental composites

AU - Caughman, Wyatt Franklin

AU - Caughman, Gretchen B

AU - Shiflett, Robert A.

AU - Rueggeberg, Frederick

AU - Schuster, George S.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - Previous studies have shown that dental resin composites tested in cell culture produce cytotoxic effects on human gingival tissues. In this study, the cytotoxic potential of resin composites on primary human gingival fibroblast cultures was evaluated, based on inhibition of cellular protein synthesis measured by [35S] methionine incorporation. Both resin content and percentage of monomer conversion were considered as potential causes of cytotoxicity. Three resin composites were selected to provide a range of filler content from 45 to 86 wt%. Duplicate sample discs (1 mm thick x 10 mm diameter) of each composite were polymerized for 15, 30 and 60 s, followed by heat (110°C, 10 min), and the degree of monomer conversion for each sample group was measured using Fourier transform infrared spectrophotometry. Identically fabricated discs were placed into 35 mm culture dishes with gingival fibroblasts and incubated for 24 h at 37°C. The cell monolayers then were labelled at 24 h with [35S] methionine, washed and solubilized; then incorporated radioactivity was quantitated by liquid scintillation spectrometry. For each composite, as the percentage of monomer conversion increased, cellular toxicity decreased. In comparing different composites having similar monomer conversions, it was found that the filler resin ratio was not the only factor determining the composite's relative toxicity.

AB - Previous studies have shown that dental resin composites tested in cell culture produce cytotoxic effects on human gingival tissues. In this study, the cytotoxic potential of resin composites on primary human gingival fibroblast cultures was evaluated, based on inhibition of cellular protein synthesis measured by [35S] methionine incorporation. Both resin content and percentage of monomer conversion were considered as potential causes of cytotoxicity. Three resin composites were selected to provide a range of filler content from 45 to 86 wt%. Duplicate sample discs (1 mm thick x 10 mm diameter) of each composite were polymerized for 15, 30 and 60 s, followed by heat (110°C, 10 min), and the degree of monomer conversion for each sample group was measured using Fourier transform infrared spectrophotometry. Identically fabricated discs were placed into 35 mm culture dishes with gingival fibroblasts and incubated for 24 h at 37°C. The cell monolayers then were labelled at 24 h with [35S] methionine, washed and solubilized; then incorporated radioactivity was quantitated by liquid scintillation spectrometry. For each composite, as the percentage of monomer conversion increased, cellular toxicity decreased. In comparing different composites having similar monomer conversions, it was found that the filler resin ratio was not the only factor determining the composite's relative toxicity.

KW - Cytotoxicity

KW - composites

KW - dental materials

UR - http://www.scopus.com/inward/record.url?scp=0025918460&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025918460&partnerID=8YFLogxK

U2 - 10.1016/0142-9612(91)90022-3

DO - 10.1016/0142-9612(91)90022-3

M3 - Article

C2 - 1799649

AN - SCOPUS:0025918460

VL - 12

SP - 737

EP - 740

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 8

ER -