CRISPR links to long noncoding RNA function in mice: A practical approach

Joseph M. Miano, Xiaochun Long, Qing Lyu

Research output: Contribution to journalReview article

Abstract

Next generation sequencing has uncovered a trove of short noncoding RNAs (e.g., microRNAs) and long noncoding RNAs (lncRNAs) that act as molecular rheostats in the control of diverse homeostatic processes. Meanwhile, the tsunamic emergence of clustered regularly interspaced short palindromic repeats (CRISPR) editing has transformed our influence over all DNA-carrying entities, heralding global CRISPRization. This is evident in biomedical research where the ease and low-cost of CRISPR editing has made it the preferred method of manipulating the mouse genome, facilitating rapid discovery of genome function in an in vivo context. Here, CRISPR genome editing components are updated for elucidating lncRNA function in mice. Various strategies are highlighted for understanding the function of lncRNAs residing in intergenic sequence space, as host genes that harbor microRNAs or other genes, and as natural antisense, overlapping or intronic genes. Also discussed is CRISPR editing of mice carrying human lncRNAs as well as the editing of competing endogenous RNAs. The information described herein should assist labs in the rigorous design of experiments that interrogate lncRNA function in mice where complex disease processes can be modeled thus accelerating translational discovery.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalVascular Pharmacology
Volume114
DOIs
StatePublished - Mar 2019
Externally publishedYes

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
Long Noncoding RNA
MicroRNAs
Genome Components
Genome
Genes
Small Untranslated RNA
Intergenic DNA
Biomedical Research
RNA
Costs and Cost Analysis
DNA

Keywords

  • CRISPR
  • Genetics
  • Genome editing
  • Long noncoding RNA
  • Mouse

ASJC Scopus subject areas

  • Physiology
  • Molecular Medicine
  • Pharmacology

Cite this

CRISPR links to long noncoding RNA function in mice : A practical approach. / Miano, Joseph M.; Long, Xiaochun; Lyu, Qing.

In: Vascular Pharmacology, Vol. 114, 03.2019, p. 1-12.

Research output: Contribution to journalReview article

Miano, Joseph M. ; Long, Xiaochun ; Lyu, Qing. / CRISPR links to long noncoding RNA function in mice : A practical approach. In: Vascular Pharmacology. 2019 ; Vol. 114. pp. 1-12.
@article{62c081c3261c46ffb21d69dfcbc900b0,
title = "CRISPR links to long noncoding RNA function in mice: A practical approach",
abstract = "Next generation sequencing has uncovered a trove of short noncoding RNAs (e.g., microRNAs) and long noncoding RNAs (lncRNAs) that act as molecular rheostats in the control of diverse homeostatic processes. Meanwhile, the tsunamic emergence of clustered regularly interspaced short palindromic repeats (CRISPR) editing has transformed our influence over all DNA-carrying entities, heralding global CRISPRization. This is evident in biomedical research where the ease and low-cost of CRISPR editing has made it the preferred method of manipulating the mouse genome, facilitating rapid discovery of genome function in an in vivo context. Here, CRISPR genome editing components are updated for elucidating lncRNA function in mice. Various strategies are highlighted for understanding the function of lncRNAs residing in intergenic sequence space, as host genes that harbor microRNAs or other genes, and as natural antisense, overlapping or intronic genes. Also discussed is CRISPR editing of mice carrying human lncRNAs as well as the editing of competing endogenous RNAs. The information described herein should assist labs in the rigorous design of experiments that interrogate lncRNA function in mice where complex disease processes can be modeled thus accelerating translational discovery.",
keywords = "CRISPR, Genetics, Genome editing, Long noncoding RNA, Mouse",
author = "Miano, {Joseph M.} and Xiaochun Long and Qing Lyu",
year = "2019",
month = "3",
doi = "10.1016/j.vph.2019.02.004",
language = "English (US)",
volume = "114",
pages = "1--12",
journal = "Vascular Pharmacology",
issn = "1537-1891",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - CRISPR links to long noncoding RNA function in mice

T2 - A practical approach

AU - Miano, Joseph M.

AU - Long, Xiaochun

AU - Lyu, Qing

PY - 2019/3

Y1 - 2019/3

N2 - Next generation sequencing has uncovered a trove of short noncoding RNAs (e.g., microRNAs) and long noncoding RNAs (lncRNAs) that act as molecular rheostats in the control of diverse homeostatic processes. Meanwhile, the tsunamic emergence of clustered regularly interspaced short palindromic repeats (CRISPR) editing has transformed our influence over all DNA-carrying entities, heralding global CRISPRization. This is evident in biomedical research where the ease and low-cost of CRISPR editing has made it the preferred method of manipulating the mouse genome, facilitating rapid discovery of genome function in an in vivo context. Here, CRISPR genome editing components are updated for elucidating lncRNA function in mice. Various strategies are highlighted for understanding the function of lncRNAs residing in intergenic sequence space, as host genes that harbor microRNAs or other genes, and as natural antisense, overlapping or intronic genes. Also discussed is CRISPR editing of mice carrying human lncRNAs as well as the editing of competing endogenous RNAs. The information described herein should assist labs in the rigorous design of experiments that interrogate lncRNA function in mice where complex disease processes can be modeled thus accelerating translational discovery.

AB - Next generation sequencing has uncovered a trove of short noncoding RNAs (e.g., microRNAs) and long noncoding RNAs (lncRNAs) that act as molecular rheostats in the control of diverse homeostatic processes. Meanwhile, the tsunamic emergence of clustered regularly interspaced short palindromic repeats (CRISPR) editing has transformed our influence over all DNA-carrying entities, heralding global CRISPRization. This is evident in biomedical research where the ease and low-cost of CRISPR editing has made it the preferred method of manipulating the mouse genome, facilitating rapid discovery of genome function in an in vivo context. Here, CRISPR genome editing components are updated for elucidating lncRNA function in mice. Various strategies are highlighted for understanding the function of lncRNAs residing in intergenic sequence space, as host genes that harbor microRNAs or other genes, and as natural antisense, overlapping or intronic genes. Also discussed is CRISPR editing of mice carrying human lncRNAs as well as the editing of competing endogenous RNAs. The information described herein should assist labs in the rigorous design of experiments that interrogate lncRNA function in mice where complex disease processes can be modeled thus accelerating translational discovery.

KW - CRISPR

KW - Genetics

KW - Genome editing

KW - Long noncoding RNA

KW - Mouse

UR - http://www.scopus.com/inward/record.url?scp=85062259620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062259620&partnerID=8YFLogxK

U2 - 10.1016/j.vph.2019.02.004

DO - 10.1016/j.vph.2019.02.004

M3 - Review article

C2 - 30822570

AN - SCOPUS:85062259620

VL - 114

SP - 1

EP - 12

JO - Vascular Pharmacology

JF - Vascular Pharmacology

SN - 1537-1891

ER -