Critical role of nadph oxidase in neuronal oxidative damage and microglia activation following traumatic brain injury

Quanguang Zhang, Melissa D. Laird, Dong Han, Khoi Nguyen, Erin Scott, Yan Dong, Krishnan Michael Dhandapani, Darrell W Brann

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Background: Oxidative stress is known to play an important role in the pathology of traumatic brain injury. Mitochondria are thought to be the major source of the damaging reactive oxygen species (ROS) following TBI. However, recent work has revealed that the membrane, via the enzyme NADPH oxidase can also generate the superoxide radical (O 2 -), and thereby potentially contribute to the oxidative stress following TBI. The current study thus addressed the potential role of NADPH oxidase in TBI. Methodology/Principal Findings: The results revealed that NADPH oxidase activity in the cerebral cortex and hippocampal CA1 region increases rapidly following controlled cortical impact in male mice, with an early peak at 1 h, followed by a secondary peak from 24-96 h after TBI. In situ localization using oxidized hydroethidine and the neuronal marker, NeuN, revealed that the O 2 - induction occurred in neurons at 1 h after TBI. Pre- or post-treatment with the NADPH oxidase inhibitor, apocynin markedly inhibited microglial activation and oxidative stress damage. Apocynin also attenuated TBI-induction of the Alzheimer's disease proteins β-amyloid and amyloid precursor protein. Finally, both pre- and post-treatment of apocynin was also shown to induce significant neuroprotection against TBI. In addition, a NOX2-specific inhibitor, gp91ds-tat was also shown to exert neuroprotection against TBI. Conclusions/Significance: As a whole, the study demonstrates that NADPH oxidase activity and superoxide production exhibit a biphasic elevation in the hippocampus and cortex following TBI, which contributes significantly to the pathology of TBI via mediation of oxidative stress damage, microglial activation, and AD protein induction in the brain following TBI.

Original languageEnglish (US)
Article numbere34504
JournalPloS one
Volume7
Issue number4
DOIs
StatePublished - Apr 2 2012

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NADPH Oxidase
neuroglia
Microglia
Oxidative stress
Brain
Oxidoreductases
Chemical activation
brain
Oxidative Stress
oxidative stress
Pathology
amyloid
Superoxides
superoxide anion
pretreatment
Amyloidogenic Proteins
Hippocampal CA1 Region
Mitochondria
proteins
Amyloid beta-Protein Precursor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Critical role of nadph oxidase in neuronal oxidative damage and microglia activation following traumatic brain injury. / Zhang, Quanguang; Laird, Melissa D.; Han, Dong; Nguyen, Khoi; Scott, Erin; Dong, Yan; Dhandapani, Krishnan Michael; Brann, Darrell W.

In: PloS one, Vol. 7, No. 4, e34504, 02.04.2012.

Research output: Contribution to journalArticle

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