Cross-linked dry bonding: A new etch-and-rinse technique

Jianfeng Zhou, Ayaka Chiba, Debora L S Scheffel, Josimeri Hebling, Kelli Agee, Junji Tagami, Jianquo Tan, Dalia Abuelenain, Manar Abu Nawareg, Ali H. Hassan, Lorenzo Breschi, Franklin R. Tay, David H. Pashley

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective To determine if acid-etched, cross-linked dentin can be dehydrated without lowering bond strength below that of cross-linked wet-bonded dentin in vitro. Methods Using extracted human third molars, control acid-etched dentin was bonded with Single Bond Plus, using either the wet- or dry-bonding technique. Experimental acid-etched dentin was treated with 5 mass% grape seed extract (GSE) in different solvents for 1 min before undergoing wet vs dry resin-dentin bonding with Single Bond Plus. Completely demineralized dentin beams were treated with 5% GSE for 0, 1 or 10 min, before measuring stiffness by 3-point flexure. Other completely demineralized beams were treated similarly and then incubated in buffer for 1 week to measure the collagen solubilization by endogenous dentin proteases. Results 24 h microtensile bond strengths (μTBS) in wet and dry controls were 53.5 ± 3.6 and 9.4 ± 1.8 MPa, respectively (p < 0.05). 5% GSE in water gave μTBS of 53.7 ± 3.4 and 39.1 ± 9.7 MPa (p < 0.05), respectively, while 5% GSE in ethanol gave μTBS of 51.2 ± 2.3 and 35.3 ± 2.0 MPa (p < 0.05). 5% GSE in 5% EtOH/95% water gave wet and dry μTBS of 53.0 ± 2.3 and 55.7 ± 5.1 MPa (p > 0.05). Cross-linking demineralized dentin with 5% GSE increased stiffness of dentin and decreased collagen degradation (p < 0.05). Significance 5% GSE pretreatment of acid-etched dentin for 1 min permits the dentin to be completely air-dried without lowering bond strength.

Original languageEnglish (US)
Pages (from-to)1124-1132
Number of pages9
JournalDental Materials
Volume32
Issue number9
DOIs
StatePublished - Sep 1 2016

Fingerprint

Grape Seed Extract
Dentin
Seed
Acids
Collagen
Stiffness
Buffers
Peptide Hydrolases
Resins
Degradation
Air
Third Molar

Keywords

  • Bond strength
  • Collagen degradation
  • Cross-linking
  • Dentin
  • Dentin bonding
  • Dentin stiffness
  • Dry bonding
  • Grape seed extract
  • Wet bonding

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Zhou, J., Chiba, A., Scheffel, D. L. S., Hebling, J., Agee, K., Tagami, J., ... Pashley, D. H. (2016). Cross-linked dry bonding: A new etch-and-rinse technique. Dental Materials, 32(9), 1124-1132. https://doi.org/10.1016/j.dental.2016.06.014

Cross-linked dry bonding : A new etch-and-rinse technique. / Zhou, Jianfeng; Chiba, Ayaka; Scheffel, Debora L S; Hebling, Josimeri; Agee, Kelli; Tagami, Junji; Tan, Jianquo; Abuelenain, Dalia; Nawareg, Manar Abu; Hassan, Ali H.; Breschi, Lorenzo; Tay, Franklin R.; Pashley, David H.

In: Dental Materials, Vol. 32, No. 9, 01.09.2016, p. 1124-1132.

Research output: Contribution to journalArticle

Zhou, J, Chiba, A, Scheffel, DLS, Hebling, J, Agee, K, Tagami, J, Tan, J, Abuelenain, D, Nawareg, MA, Hassan, AH, Breschi, L, Tay, FR & Pashley, DH 2016, 'Cross-linked dry bonding: A new etch-and-rinse technique', Dental Materials, vol. 32, no. 9, pp. 1124-1132. https://doi.org/10.1016/j.dental.2016.06.014
Zhou J, Chiba A, Scheffel DLS, Hebling J, Agee K, Tagami J et al. Cross-linked dry bonding: A new etch-and-rinse technique. Dental Materials. 2016 Sep 1;32(9):1124-1132. https://doi.org/10.1016/j.dental.2016.06.014
Zhou, Jianfeng ; Chiba, Ayaka ; Scheffel, Debora L S ; Hebling, Josimeri ; Agee, Kelli ; Tagami, Junji ; Tan, Jianquo ; Abuelenain, Dalia ; Nawareg, Manar Abu ; Hassan, Ali H. ; Breschi, Lorenzo ; Tay, Franklin R. ; Pashley, David H. / Cross-linked dry bonding : A new etch-and-rinse technique. In: Dental Materials. 2016 ; Vol. 32, No. 9. pp. 1124-1132.
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abstract = "Objective To determine if acid-etched, cross-linked dentin can be dehydrated without lowering bond strength below that of cross-linked wet-bonded dentin in vitro. Methods Using extracted human third molars, control acid-etched dentin was bonded with Single Bond Plus, using either the wet- or dry-bonding technique. Experimental acid-etched dentin was treated with 5 mass{\%} grape seed extract (GSE) in different solvents for 1 min before undergoing wet vs dry resin-dentin bonding with Single Bond Plus. Completely demineralized dentin beams were treated with 5{\%} GSE for 0, 1 or 10 min, before measuring stiffness by 3-point flexure. Other completely demineralized beams were treated similarly and then incubated in buffer for 1 week to measure the collagen solubilization by endogenous dentin proteases. Results 24 h microtensile bond strengths (μTBS) in wet and dry controls were 53.5 ± 3.6 and 9.4 ± 1.8 MPa, respectively (p < 0.05). 5{\%} GSE in water gave μTBS of 53.7 ± 3.4 and 39.1 ± 9.7 MPa (p < 0.05), respectively, while 5{\%} GSE in ethanol gave μTBS of 51.2 ± 2.3 and 35.3 ± 2.0 MPa (p < 0.05). 5{\%} GSE in 5{\%} EtOH/95{\%} water gave wet and dry μTBS of 53.0 ± 2.3 and 55.7 ± 5.1 MPa (p > 0.05). Cross-linking demineralized dentin with 5{\%} GSE increased stiffness of dentin and decreased collagen degradation (p < 0.05). Significance 5{\%} GSE pretreatment of acid-etched dentin for 1 min permits the dentin to be completely air-dried without lowering bond strength.",
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AU - Hebling, Josimeri

AU - Agee, Kelli

AU - Tagami, Junji

AU - Tan, Jianquo

AU - Abuelenain, Dalia

AU - Nawareg, Manar Abu

AU - Hassan, Ali H.

AU - Breschi, Lorenzo

AU - Tay, Franklin R.

AU - Pashley, David H.

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N2 - Objective To determine if acid-etched, cross-linked dentin can be dehydrated without lowering bond strength below that of cross-linked wet-bonded dentin in vitro. Methods Using extracted human third molars, control acid-etched dentin was bonded with Single Bond Plus, using either the wet- or dry-bonding technique. Experimental acid-etched dentin was treated with 5 mass% grape seed extract (GSE) in different solvents for 1 min before undergoing wet vs dry resin-dentin bonding with Single Bond Plus. Completely demineralized dentin beams were treated with 5% GSE for 0, 1 or 10 min, before measuring stiffness by 3-point flexure. Other completely demineralized beams were treated similarly and then incubated in buffer for 1 week to measure the collagen solubilization by endogenous dentin proteases. Results 24 h microtensile bond strengths (μTBS) in wet and dry controls were 53.5 ± 3.6 and 9.4 ± 1.8 MPa, respectively (p < 0.05). 5% GSE in water gave μTBS of 53.7 ± 3.4 and 39.1 ± 9.7 MPa (p < 0.05), respectively, while 5% GSE in ethanol gave μTBS of 51.2 ± 2.3 and 35.3 ± 2.0 MPa (p < 0.05). 5% GSE in 5% EtOH/95% water gave wet and dry μTBS of 53.0 ± 2.3 and 55.7 ± 5.1 MPa (p > 0.05). Cross-linking demineralized dentin with 5% GSE increased stiffness of dentin and decreased collagen degradation (p < 0.05). Significance 5% GSE pretreatment of acid-etched dentin for 1 min permits the dentin to be completely air-dried without lowering bond strength.

AB - Objective To determine if acid-etched, cross-linked dentin can be dehydrated without lowering bond strength below that of cross-linked wet-bonded dentin in vitro. Methods Using extracted human third molars, control acid-etched dentin was bonded with Single Bond Plus, using either the wet- or dry-bonding technique. Experimental acid-etched dentin was treated with 5 mass% grape seed extract (GSE) in different solvents for 1 min before undergoing wet vs dry resin-dentin bonding with Single Bond Plus. Completely demineralized dentin beams were treated with 5% GSE for 0, 1 or 10 min, before measuring stiffness by 3-point flexure. Other completely demineralized beams were treated similarly and then incubated in buffer for 1 week to measure the collagen solubilization by endogenous dentin proteases. Results 24 h microtensile bond strengths (μTBS) in wet and dry controls were 53.5 ± 3.6 and 9.4 ± 1.8 MPa, respectively (p < 0.05). 5% GSE in water gave μTBS of 53.7 ± 3.4 and 39.1 ± 9.7 MPa (p < 0.05), respectively, while 5% GSE in ethanol gave μTBS of 51.2 ± 2.3 and 35.3 ± 2.0 MPa (p < 0.05). 5% GSE in 5% EtOH/95% water gave wet and dry μTBS of 53.0 ± 2.3 and 55.7 ± 5.1 MPa (p > 0.05). Cross-linking demineralized dentin with 5% GSE increased stiffness of dentin and decreased collagen degradation (p < 0.05). Significance 5% GSE pretreatment of acid-etched dentin for 1 min permits the dentin to be completely air-dried without lowering bond strength.

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KW - Collagen degradation

KW - Cross-linking

KW - Dentin

KW - Dentin bonding

KW - Dentin stiffness

KW - Dry bonding

KW - Grape seed extract

KW - Wet bonding

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