Cross-linking effect on dentin bond strength and MMPs activity

Annalisa Mazzoni, Valeria Angeloni, Allegra Comba, Tatjana Maravic, Milena Cadenaro, Arzu Tezvergil-Mutluay, David Henry Pashley, Franklin Chi Meng Tay, Lorenzo Breschi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: The objectives of the study were to evaluate the ability of a 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide (EDC)-containing primer to improve immediate bond strength of either self-etch or etch-and-rinse adhesive systems and to stabilize the adhesive interfaces over time. A further objective was to investigate the effect of EDC on the dentinal MMPs activity using zymographic analysis. Methods: Freshly extracted molars (n = 80, 20 for each group) were selected to conduct microtensile bond strength tests. The following groups were tested, immediately or after 1-year aging in artificial saliva: G1: Clearfil SE (CSE) primer applied on unetched dentin, pretreated with 0.3 M EDC water-solution for 1 min and bonded with CSE Bond; G2: as G1 but without EDC pre-treatment; G3: acid-etched (35% phosphoric-acid for 15s) dentin pretreated with 0.3 M EDC, then bonded with XP Bond (XPB); Group 4 (G4): as G3 without EDC pre-treatment. Further, gelatinase activity in dentin powder treated with CSE and XPB with and without EDC pre-treatment, was analyzed using gelatin zymography. Results: The use of 0.3 M EDC-containing conditioner did not affect the immediate bond strength of XPB or CSE adhesive systems (p > 0.05), while it improved the bond strength after 1 year of aging (p < 0.05). Pre-treatment with EDC followed by the application of CSE resulted in an incomplete MMPs inactivation, while EDC pretreatment followed by the application of XPB resulted in an almost complete inactivation of dentinal gelatinases. Significance: The μTBS and zymography results support the efficacy of EDC over time and reveal that changes within the dentin matrix promoted by EDC are not adhesive-system-dependent.

Original languageEnglish (US)
Pages (from-to)288-295
Number of pages8
JournalDental Materials
Volume34
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Dentin
Matrix Metalloproteinases
Adhesives
Gelatinases
Aging of materials
Ethyldimethylaminopropyl Carbodiimide
Artificial Saliva
Phosphoric acid
Gelatin
Powders
Acids
XP-Bond
Water
Clearfil SE Bond

Keywords

  • Dentin bonding systems
  • EDC
  • MMPs
  • Protein cross-linkers

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Mazzoni, A., Angeloni, V., Comba, A., Maravic, T., Cadenaro, M., Tezvergil-Mutluay, A., ... Breschi, L. (2018). Cross-linking effect on dentin bond strength and MMPs activity. Dental Materials, 34(2), 288-295. https://doi.org/10.1016/j.dental.2017.11.009

Cross-linking effect on dentin bond strength and MMPs activity. / Mazzoni, Annalisa; Angeloni, Valeria; Comba, Allegra; Maravic, Tatjana; Cadenaro, Milena; Tezvergil-Mutluay, Arzu; Pashley, David Henry; Tay, Franklin Chi Meng; Breschi, Lorenzo.

In: Dental Materials, Vol. 34, No. 2, 01.02.2018, p. 288-295.

Research output: Contribution to journalArticle

Mazzoni, A, Angeloni, V, Comba, A, Maravic, T, Cadenaro, M, Tezvergil-Mutluay, A, Pashley, DH, Tay, FCM & Breschi, L 2018, 'Cross-linking effect on dentin bond strength and MMPs activity', Dental Materials, vol. 34, no. 2, pp. 288-295. https://doi.org/10.1016/j.dental.2017.11.009
Mazzoni A, Angeloni V, Comba A, Maravic T, Cadenaro M, Tezvergil-Mutluay A et al. Cross-linking effect on dentin bond strength and MMPs activity. Dental Materials. 2018 Feb 1;34(2):288-295. https://doi.org/10.1016/j.dental.2017.11.009
Mazzoni, Annalisa ; Angeloni, Valeria ; Comba, Allegra ; Maravic, Tatjana ; Cadenaro, Milena ; Tezvergil-Mutluay, Arzu ; Pashley, David Henry ; Tay, Franklin Chi Meng ; Breschi, Lorenzo. / Cross-linking effect on dentin bond strength and MMPs activity. In: Dental Materials. 2018 ; Vol. 34, No. 2. pp. 288-295.
@article{f5c9458358a044e5b1ce09e3e6f7d8cd,
title = "Cross-linking effect on dentin bond strength and MMPs activity",
abstract = "Objective: The objectives of the study were to evaluate the ability of a 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide (EDC)-containing primer to improve immediate bond strength of either self-etch or etch-and-rinse adhesive systems and to stabilize the adhesive interfaces over time. A further objective was to investigate the effect of EDC on the dentinal MMPs activity using zymographic analysis. Methods: Freshly extracted molars (n = 80, 20 for each group) were selected to conduct microtensile bond strength tests. The following groups were tested, immediately or after 1-year aging in artificial saliva: G1: Clearfil SE (CSE) primer applied on unetched dentin, pretreated with 0.3 M EDC water-solution for 1 min and bonded with CSE Bond; G2: as G1 but without EDC pre-treatment; G3: acid-etched (35{\%} phosphoric-acid for 15s) dentin pretreated with 0.3 M EDC, then bonded with XP Bond (XPB); Group 4 (G4): as G3 without EDC pre-treatment. Further, gelatinase activity in dentin powder treated with CSE and XPB with and without EDC pre-treatment, was analyzed using gelatin zymography. Results: The use of 0.3 M EDC-containing conditioner did not affect the immediate bond strength of XPB or CSE adhesive systems (p > 0.05), while it improved the bond strength after 1 year of aging (p < 0.05). Pre-treatment with EDC followed by the application of CSE resulted in an incomplete MMPs inactivation, while EDC pretreatment followed by the application of XPB resulted in an almost complete inactivation of dentinal gelatinases. Significance: The μTBS and zymography results support the efficacy of EDC over time and reveal that changes within the dentin matrix promoted by EDC are not adhesive-system-dependent.",
keywords = "Dentin bonding systems, EDC, MMPs, Protein cross-linkers",
author = "Annalisa Mazzoni and Valeria Angeloni and Allegra Comba and Tatjana Maravic and Milena Cadenaro and Arzu Tezvergil-Mutluay and Pashley, {David Henry} and Tay, {Franklin Chi Meng} and Lorenzo Breschi",
year = "2018",
month = "2",
day = "1",
doi = "10.1016/j.dental.2017.11.009",
language = "English (US)",
volume = "34",
pages = "288--295",
journal = "Dental Materials",
issn = "0109-5641",
publisher = "Elsevier Science",
number = "2",

}

TY - JOUR

T1 - Cross-linking effect on dentin bond strength and MMPs activity

AU - Mazzoni, Annalisa

AU - Angeloni, Valeria

AU - Comba, Allegra

AU - Maravic, Tatjana

AU - Cadenaro, Milena

AU - Tezvergil-Mutluay, Arzu

AU - Pashley, David Henry

AU - Tay, Franklin Chi Meng

AU - Breschi, Lorenzo

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Objective: The objectives of the study were to evaluate the ability of a 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide (EDC)-containing primer to improve immediate bond strength of either self-etch or etch-and-rinse adhesive systems and to stabilize the adhesive interfaces over time. A further objective was to investigate the effect of EDC on the dentinal MMPs activity using zymographic analysis. Methods: Freshly extracted molars (n = 80, 20 for each group) were selected to conduct microtensile bond strength tests. The following groups were tested, immediately or after 1-year aging in artificial saliva: G1: Clearfil SE (CSE) primer applied on unetched dentin, pretreated with 0.3 M EDC water-solution for 1 min and bonded with CSE Bond; G2: as G1 but without EDC pre-treatment; G3: acid-etched (35% phosphoric-acid for 15s) dentin pretreated with 0.3 M EDC, then bonded with XP Bond (XPB); Group 4 (G4): as G3 without EDC pre-treatment. Further, gelatinase activity in dentin powder treated with CSE and XPB with and without EDC pre-treatment, was analyzed using gelatin zymography. Results: The use of 0.3 M EDC-containing conditioner did not affect the immediate bond strength of XPB or CSE adhesive systems (p > 0.05), while it improved the bond strength after 1 year of aging (p < 0.05). Pre-treatment with EDC followed by the application of CSE resulted in an incomplete MMPs inactivation, while EDC pretreatment followed by the application of XPB resulted in an almost complete inactivation of dentinal gelatinases. Significance: The μTBS and zymography results support the efficacy of EDC over time and reveal that changes within the dentin matrix promoted by EDC are not adhesive-system-dependent.

AB - Objective: The objectives of the study were to evaluate the ability of a 1-ethyl-3 (3-dimethylaminopropyl) carbodiimide (EDC)-containing primer to improve immediate bond strength of either self-etch or etch-and-rinse adhesive systems and to stabilize the adhesive interfaces over time. A further objective was to investigate the effect of EDC on the dentinal MMPs activity using zymographic analysis. Methods: Freshly extracted molars (n = 80, 20 for each group) were selected to conduct microtensile bond strength tests. The following groups were tested, immediately or after 1-year aging in artificial saliva: G1: Clearfil SE (CSE) primer applied on unetched dentin, pretreated with 0.3 M EDC water-solution for 1 min and bonded with CSE Bond; G2: as G1 but without EDC pre-treatment; G3: acid-etched (35% phosphoric-acid for 15s) dentin pretreated with 0.3 M EDC, then bonded with XP Bond (XPB); Group 4 (G4): as G3 without EDC pre-treatment. Further, gelatinase activity in dentin powder treated with CSE and XPB with and without EDC pre-treatment, was analyzed using gelatin zymography. Results: The use of 0.3 M EDC-containing conditioner did not affect the immediate bond strength of XPB or CSE adhesive systems (p > 0.05), while it improved the bond strength after 1 year of aging (p < 0.05). Pre-treatment with EDC followed by the application of CSE resulted in an incomplete MMPs inactivation, while EDC pretreatment followed by the application of XPB resulted in an almost complete inactivation of dentinal gelatinases. Significance: The μTBS and zymography results support the efficacy of EDC over time and reveal that changes within the dentin matrix promoted by EDC are not adhesive-system-dependent.

KW - Dentin bonding systems

KW - EDC

KW - MMPs

KW - Protein cross-linkers

UR - http://www.scopus.com/inward/record.url?scp=85035105529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85035105529&partnerID=8YFLogxK

U2 - 10.1016/j.dental.2017.11.009

DO - 10.1016/j.dental.2017.11.009

M3 - Article

C2 - 29179972

AN - SCOPUS:85035105529

VL - 34

SP - 288

EP - 295

JO - Dental Materials

JF - Dental Materials

SN - 0109-5641

IS - 2

ER -