Culture media and their components differ their ability to scavenge reactive oxygen species in the plasmid relaxation assay

Alexander Ermilov, Michael Peter Diamond, Anthony G. Sacco, Dmitri D. Dozortsev

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Objective: To investigate the modulation of DNA-damaging effects of reactive oxygen species by media composition. Design: In vitro study. Setting: Academic medical center. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): Plasmid relaxation. Result(s): Ham's F-10 medium, 1% Percoll, superoxide dismutase (1, 10, or 100 IU), and synthetic serum substitute did not affect DNA damage by reactive oxygen species and did not have any effect on plasmid DNA damage. Plasmid DNA damage was partially inhibited in the presence of P-1 and human tubal fluid media. Human serum albumin, phenol red, glucose, polyvinyl alcohol, polyvinylpyrrolidone, sucrose, and HEPES also were found to protect DNA from damage. Conclusion(s): In vitro fertilization media and their components vary widely in the way they affect DNA damage by reactive oxygen species.

Original languageEnglish (US)
Pages (from-to)154-157
Number of pages4
JournalFertility and Sterility
Volume72
Issue number1
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

DNA Damage
Culture Media
Reactive Oxygen Species
Plasmids
Phenolsulfonphthalein
HEPES
Polyvinyl Alcohol
Povidone
Fertilization in Vitro
Serum Albumin
Sucrose
Outcome Assessment (Health Care)
Glucose
DNA
Serum

Keywords

  • DNA damage
  • In vitro fertilization
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

Culture media and their components differ their ability to scavenge reactive oxygen species in the plasmid relaxation assay. / Ermilov, Alexander; Diamond, Michael Peter; Sacco, Anthony G.; Dozortsev, Dmitri D.

In: Fertility and Sterility, Vol. 72, No. 1, 01.01.1999, p. 154-157.

Research output: Contribution to journalArticle

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