Culture of dissociated hippocampal neurons

Yun Peng, Wen Cheng Xiong, Lin Mei

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


Hippocampal neurons consist mainly of pyramidal neuron and granule cell, and dissociated hippocampal neurons are a good tool to investigate the molecular and cellular mechanism of neuronal development and neuronal degenerative disease in the central neuronal system (CNS). Here, we describe a general procedure of dissociated hippocampal neuron culture.

Original languageEnglish (US)
Title of host publicationNeural Development
Subtitle of host publicationMethods and Protocols
Number of pages9
StatePublished - Sep 4 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Coating
  • Culture
  • Digestion
  • Dissection
  • Dissociated neuron
  • Feeding
  • Glial inhibition
  • Hippocampus
  • Plating

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Peng, Y., Xiong, W. C., & Mei, L. (2013). Culture of dissociated hippocampal neurons. In Neural Development: Methods and Protocols (pp. 39-47). (Methods in Molecular Biology; Vol. 1018).