Culture of dissociated hippocampal neurons

Yun Peng, Wen Cheng Xiong, Lin Mei

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Hippocampal neurons consist mainly of pyramidal neuron and granule cell, and dissociated hippocampal neurons are a good tool to investigate the molecular and cellular mechanism of neuronal development and neuronal degenerative disease in the central neuronal system (CNS). Here, we describe a general procedure of dissociated hippocampal neuron culture.

Original languageEnglish (US)
Title of host publicationNeural Development
Subtitle of host publicationMethods and Protocols
Pages39-47
Number of pages9
DOIs
StatePublished - Sep 4 2013

Publication series

NameMethods in Molecular Biology
Volume1018
ISSN (Print)1064-3745

Fingerprint

Neurons
Pyramidal Cells

Keywords

  • Coating
  • Culture
  • Digestion
  • Dissection
  • Dissociated neuron
  • Feeding
  • Glial inhibition
  • Hippocampus
  • Plating

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Peng, Y., Xiong, W. C., & Mei, L. (2013). Culture of dissociated hippocampal neurons. In Neural Development: Methods and Protocols (pp. 39-47). (Methods in Molecular Biology; Vol. 1018). https://doi.org/10.1007/978-1-62703-444-9-4

Culture of dissociated hippocampal neurons. / Peng, Yun; Xiong, Wen Cheng; Mei, Lin.

Neural Development: Methods and Protocols. 2013. p. 39-47 (Methods in Molecular Biology; Vol. 1018).

Research output: Chapter in Book/Report/Conference proceedingChapter

Peng, Y, Xiong, WC & Mei, L 2013, Culture of dissociated hippocampal neurons. in Neural Development: Methods and Protocols. Methods in Molecular Biology, vol. 1018, pp. 39-47. https://doi.org/10.1007/978-1-62703-444-9-4
Peng Y, Xiong WC, Mei L. Culture of dissociated hippocampal neurons. In Neural Development: Methods and Protocols. 2013. p. 39-47. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-444-9-4
Peng, Yun ; Xiong, Wen Cheng ; Mei, Lin. / Culture of dissociated hippocampal neurons. Neural Development: Methods and Protocols. 2013. pp. 39-47 (Methods in Molecular Biology).
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