Culture of prostatic epithelial cells from ultrasound‐guided needle biopsies

Donna M. Peehl, Stephen T. Wong, Martha Kennedy Terris, Thomas A. Stamey

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A protocol which was developed for the culture of epithelial cells from radical prostatectomy specimens was slightly modified to permit the culture of cells from ultrasound‐guided prostatic needle biopsies. The collagenase digestion step of the standard protocol was omitted, and biopsies were simply minced and allowed to attach to collagen‐coated dishes in serum‐free medium. Cell outgrowths from biopsies were free of fibroblasts, and expression of keratin, prostate specific antigen, and prostatic acid phosphatase was maintained in vitro. The establishment of a bank of frozen cells from primary cultures permitted repetitive studies with individual cell strains, which could be serially passaged and were capable of clonal growth. The ability to derive cultures from biopsies will facilitate the biological characterization of cells from primary prostate tumors of high malignant grade, which are not commonly available from radical prostatectomy specimens.

Original languageEnglish (US)
Pages (from-to)141-147
Number of pages7
JournalThe Prostate
Volume19
Issue number2
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Needle Biopsy
Epithelial Cells
Prostatectomy
Biopsy
Primary Cell Culture
Collagenases
Prostate-Specific Antigen
Keratins
Prostate
Digestion
Cell Culture Techniques
Fibroblasts
Growth
Neoplasms

Keywords

  • cancer
  • prostate

ASJC Scopus subject areas

  • Oncology
  • Urology

Cite this

Culture of prostatic epithelial cells from ultrasound‐guided needle biopsies. / Peehl, Donna M.; Wong, Stephen T.; Terris, Martha Kennedy; Stamey, Thomas A.

In: The Prostate, Vol. 19, No. 2, 01.01.1991, p. 141-147.

Research output: Contribution to journalArticle

Peehl, Donna M. ; Wong, Stephen T. ; Terris, Martha Kennedy ; Stamey, Thomas A. / Culture of prostatic epithelial cells from ultrasound‐guided needle biopsies. In: The Prostate. 1991 ; Vol. 19, No. 2. pp. 141-147.
@article{d47d31d2f5f64678870f4d1e28f83860,
title = "Culture of prostatic epithelial cells from ultrasound‐guided needle biopsies",
abstract = "A protocol which was developed for the culture of epithelial cells from radical prostatectomy specimens was slightly modified to permit the culture of cells from ultrasound‐guided prostatic needle biopsies. The collagenase digestion step of the standard protocol was omitted, and biopsies were simply minced and allowed to attach to collagen‐coated dishes in serum‐free medium. Cell outgrowths from biopsies were free of fibroblasts, and expression of keratin, prostate specific antigen, and prostatic acid phosphatase was maintained in vitro. The establishment of a bank of frozen cells from primary cultures permitted repetitive studies with individual cell strains, which could be serially passaged and were capable of clonal growth. The ability to derive cultures from biopsies will facilitate the biological characterization of cells from primary prostate tumors of high malignant grade, which are not commonly available from radical prostatectomy specimens.",
keywords = "cancer, prostate",
author = "Peehl, {Donna M.} and Wong, {Stephen T.} and Terris, {Martha Kennedy} and Stamey, {Thomas A.}",
year = "1991",
month = "1",
day = "1",
doi = "10.1002/pros.2990190207",
language = "English (US)",
volume = "19",
pages = "141--147",
journal = "Prostate",
issn = "0270-4137",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Culture of prostatic epithelial cells from ultrasound‐guided needle biopsies

AU - Peehl, Donna M.

AU - Wong, Stephen T.

AU - Terris, Martha Kennedy

AU - Stamey, Thomas A.

PY - 1991/1/1

Y1 - 1991/1/1

N2 - A protocol which was developed for the culture of epithelial cells from radical prostatectomy specimens was slightly modified to permit the culture of cells from ultrasound‐guided prostatic needle biopsies. The collagenase digestion step of the standard protocol was omitted, and biopsies were simply minced and allowed to attach to collagen‐coated dishes in serum‐free medium. Cell outgrowths from biopsies were free of fibroblasts, and expression of keratin, prostate specific antigen, and prostatic acid phosphatase was maintained in vitro. The establishment of a bank of frozen cells from primary cultures permitted repetitive studies with individual cell strains, which could be serially passaged and were capable of clonal growth. The ability to derive cultures from biopsies will facilitate the biological characterization of cells from primary prostate tumors of high malignant grade, which are not commonly available from radical prostatectomy specimens.

AB - A protocol which was developed for the culture of epithelial cells from radical prostatectomy specimens was slightly modified to permit the culture of cells from ultrasound‐guided prostatic needle biopsies. The collagenase digestion step of the standard protocol was omitted, and biopsies were simply minced and allowed to attach to collagen‐coated dishes in serum‐free medium. Cell outgrowths from biopsies were free of fibroblasts, and expression of keratin, prostate specific antigen, and prostatic acid phosphatase was maintained in vitro. The establishment of a bank of frozen cells from primary cultures permitted repetitive studies with individual cell strains, which could be serially passaged and were capable of clonal growth. The ability to derive cultures from biopsies will facilitate the biological characterization of cells from primary prostate tumors of high malignant grade, which are not commonly available from radical prostatectomy specimens.

KW - cancer

KW - prostate

UR - http://www.scopus.com/inward/record.url?scp=0026040669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026040669&partnerID=8YFLogxK

U2 - 10.1002/pros.2990190207

DO - 10.1002/pros.2990190207

M3 - Article

C2 - 1717964

AN - SCOPUS:0026040669

VL - 19

SP - 141

EP - 147

JO - Prostate

JF - Prostate

SN - 0270-4137

IS - 2

ER -