Curcumin improves the tumoricidal effect of mitomycin C by suppressing ABCG2 expression in stem cell-like breast cancer cells

Qianmei Zhou, Meina Ye, Yiyu Lu, Hui Zhang, Qilong Chen, Shuang Huang, Shibing Su

Research output: Contribution to journalArticle

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Abstract

Cancer cells with stem cell-like properties contribute to the development of resistance to chemotherapy and eventually to tumor relapses. The current study investigated the potential of curcumin to reduce breast cancer stem cell (BCSC) population for sensitizing breast cancer cells to mitomycin C (MMC) both in vitro and in vivo. Curcumin improved the sensitivity of paclitaxel, cisplatin, and doxorubicin in breast cancer cell lines MCF-7 and MDA-MB-231, as shown by the more than 2-fold decrease in the half-maximal inhibitory concentration of these chemotherapeutic agents. In addition, curcumin sensitized the BCSCs of MCF-7 and MDA-MB-231 to MMC by 5-and 15-fold, respectively. The BCSCs could not grow to the fifth generation in the presence of curcumin and MMC. MMC or curcumin alone only marginally reduced the BCSC population in the mammospheres; however, together, they reduced the BCSC population in CD44+CD24-/low cells by more than 75% (29.34% to 6.86%). Curcumin sensitized BCSCs through a reduction in the expression of ATP-binding cassette (ABC) transporters ABCG2 and ABCC1. We demonstrated that fumitremorgin C, a selective ABCG2 inhibitor, reduced BCSC survival to a similar degree as curcumin did. Curcumin sensitized breast cancer cells to chemotherapeutic drugs by reducing the BCSC population mainly through a reduction in the expression of ABCG2.

Original languageEnglish (US)
Article numbere0136694
JournalPLoS One
Volume10
Issue number8
DOIs
StatePublished - Aug 25 2015

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mitomycin
Curcumin
curcumin
Mitomycin
Stem cells
breast neoplasms
stem cells
Stem Cells
Cells
Neoplastic Stem Cells
Breast Neoplasms
Population
ATP-Binding Cassette Transporters
Chemotherapy
neoplasm cells
ABC transporters
cisplatin
doxorubicin
paclitaxel
Paclitaxel

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Curcumin improves the tumoricidal effect of mitomycin C by suppressing ABCG2 expression in stem cell-like breast cancer cells. / Zhou, Qianmei; Ye, Meina; Lu, Yiyu; Zhang, Hui; Chen, Qilong; Huang, Shuang; Su, Shibing.

In: PLoS One, Vol. 10, No. 8, e0136694, 25.08.2015.

Research output: Contribution to journalArticle

Zhou, Qianmei ; Ye, Meina ; Lu, Yiyu ; Zhang, Hui ; Chen, Qilong ; Huang, Shuang ; Su, Shibing. / Curcumin improves the tumoricidal effect of mitomycin C by suppressing ABCG2 expression in stem cell-like breast cancer cells. In: PLoS One. 2015 ; Vol. 10, No. 8.
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