Curcumin-loaded embryonic stem cell exosomes restored neurovascular unit following ischemia-reperfusion injury

Anuradha Kalani, Pankaj Chaturvedi, Pradip K. Kamat, Claudio Maldonado, Philip Bauer, Irving G. Joshua, Suresh C. Tyagi, Neetu Tyagi

Research output: Contribution to journalArticlepeer-review

74 Scopus citations

Abstract

We tested whether the combined nano-formulation, prepared with curcumin (anti-inflammatory and neuroprotective molecule) and embryonic stem cell exosomes (MESC-exocur), restored neurovascular loss following an ischemia reperfusion (IR) injury in mice. IR-injury was created in 8–10 weeks old mice and divided into two groups. Out of two IR-injured groups, one group received intranasal administration of MESC-exocur for 7 days. Similarly, two sham groups were made and one group received MESC-exocur treatment. The study determined that MESC-exocur treatment reduced neurological score, infarct volume and edema following IR-injury. As compared to untreated IR group, MESC-exocur treated-IR group showed reduced inflammation and N-methyl-D-aspartate receptor expression. Treatment of MESC-exocur also reduced astrocytic GFAP expression and alleviated the expression of NeuN positive neurons in IR-injured mice. In addition, MESC-exocur treatment restored vascular endothelial tight (claudin-5 and occludin) and adherent (VE-cadherin) junction proteins in IR-injured mice as compared to untreated IR-injured mice. These results suggest that combining the potentials of embryonic stem cell exosomes and curcumin can help neurovascular restoration following ischemia-reperfusion injury in mice.

Original languageEnglish (US)
Pages (from-to)360-369
Number of pages10
JournalInternational Journal of Biochemistry and Cell Biology
Volume79
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

Keywords

  • Curcumin
  • Exosomes
  • Neurons
  • Stroke

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

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