Cutting edge: Activation of NK T cells by CD1d and α-galactosylceramide directs conventional T cells to the acquisition of a Th2 phenotype

Nagendra Singh, Seokmann Hong, David C. Scherer, Isao Serizawa, Nicolas Burdin, Mitchell Kronenberg, Yasuhiko Koezuka, Luc Van Kaer

Research output: Contribution to journalArticle

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Abstract

NK T cells recognize glycolipid Ags such as α-galactosylceramide (α- GalCer) presented by the MHC class I-like molecule CD1d. In this paper we have studied the in vivo effects of α-GalCer on the generation of adaptive immune responses. Treatment of mice with α-GalCer resulted in rapid activation of NK T cells and production of the cytokines IL-4 and IFN-γ. However, after this initial stimulation, NK T cells became polarized for the production of IL-4. Further, as soon as 6 days after α-GalCer injection, a marked increase in serum IgE levels was observed. Administration of α-GalCer at the time of priming of mice with protein Ag resulted in the generation of Ag-specific Th2 cells and a profound increase in the production of IgE. Collectively, these findings indicate that α-GalCer may be useful for modulating immune responses toward a Th2 phenotype during prophylaxis and therapy.

Original languageEnglish (US)
Pages (from-to)2373-2377
Number of pages5
JournalJournal of Immunology
Volume163
Issue number5
StatePublished - Sep 1 1999

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Galactosylceramides
Natural Killer Cells
T-Lymphocytes
Phenotype
Interleukin-4
Immunoglobulin E
CD1d Antigen
Th2 Cells
Glycolipids
Adaptive Immunity
Cytokines
Injections
Therapeutics
Serum
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Singh, N., Hong, S., Scherer, D. C., Serizawa, I., Burdin, N., Kronenberg, M., ... Van Kaer, L. (1999). Cutting edge: Activation of NK T cells by CD1d and α-galactosylceramide directs conventional T cells to the acquisition of a Th2 phenotype. Journal of Immunology, 163(5), 2373-2377.

Cutting edge : Activation of NK T cells by CD1d and α-galactosylceramide directs conventional T cells to the acquisition of a Th2 phenotype. / Singh, Nagendra; Hong, Seokmann; Scherer, David C.; Serizawa, Isao; Burdin, Nicolas; Kronenberg, Mitchell; Koezuka, Yasuhiko; Van Kaer, Luc.

In: Journal of Immunology, Vol. 163, No. 5, 01.09.1999, p. 2373-2377.

Research output: Contribution to journalArticle

Singh, N, Hong, S, Scherer, DC, Serizawa, I, Burdin, N, Kronenberg, M, Koezuka, Y & Van Kaer, L 1999, 'Cutting edge: Activation of NK T cells by CD1d and α-galactosylceramide directs conventional T cells to the acquisition of a Th2 phenotype', Journal of Immunology, vol. 163, no. 5, pp. 2373-2377.
Singh, Nagendra ; Hong, Seokmann ; Scherer, David C. ; Serizawa, Isao ; Burdin, Nicolas ; Kronenberg, Mitchell ; Koezuka, Yasuhiko ; Van Kaer, Luc. / Cutting edge : Activation of NK T cells by CD1d and α-galactosylceramide directs conventional T cells to the acquisition of a Th2 phenotype. In: Journal of Immunology. 1999 ; Vol. 163, No. 5. pp. 2373-2377.
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