Cyclic amp enhances toxicity of tumor necrosis factor alpha (tnf-α) in l929 cells

S. K. Leeper-Woodfonl, L. M. Zhane, Manuel R Castresana, W. H. Newman

Research output: Contribution to journalArticle

Abstract

Mouse L929 cells undergo necrosis in response to TNF-α and are used as a standard assay for biologically active TNF-α. Therefore determining the effect of agents on TNF-α cytotoxicity in this cell line could contribute to understanding the mechanism of cell death and also is important because of consequences in a standard assay. L929 cells were grown to confluence in 96 well plates. Human recombinant TNF-α was added in increasing concentrations (12.5 pg/ml to 400 pg/ml) for 24 hr. and cytotoxic activity was determined by staining cells with crystal violet. The influence of 8-Br-cAMP on TNF-α cytotoxity was also determined Intracellular CAMP was measured by RIA. L929 cells were killed by TNF-α dependent on concentration. Maximum % cytotoxity was 60 ± 0.1% at 400 pg/ml TNF-α. 8-Br-cAMP, alone, produced no cytotoxicity. However, the addition of 0.1, 0.5, or, 1 mM 8-Br-cAMP to the medium significantly shifted the TNF-α cytotoxicity curve upward and to the left in a concentration-dependent manner. Maximum cytotoxicity of TNF was increased to 74 ± 0.1% with 1 mM 8-Br-cAMP (P < 0.05) and 50% cytotoxicity occurred at 25 pg/ml TNF vs. 200 pg/ml in the absence of 8-Br-cAMP. Intracellular CAMP was 8 4 ±0.5 pmol/mg cell protein in control cells and increased to 17.4 ±1.5 (p < 0.05) in cells exposed to 1 mM 8-Br-cAMP Forskolin also potentiated cytotoxicity with concentrations of TNF-α up to 100 pg/ml but did not alter maximum cytotoxicity. These results suggest that elevated Intracellular cAMP potentiates the cytotoxic effect of TNF-α in L929 cells. Such findings suggest caution when using the L929 cell cytotoxicity assay for TNF-α in situations where alterations in cAMP might be encountered.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996
Externally publishedYes

Fingerprint

Cytotoxicity
Toxicity
Tumor Necrosis Factor-alpha
Assays
Gentian Violet
Colforsin
Cell death
Cells
Cell Death
Necrosis
Staining and Labeling
Cell Line

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Cyclic amp enhances toxicity of tumor necrosis factor alpha (tnf-α) in l929 cells. / Leeper-Woodfonl, S. K.; Zhane, L. M.; Castresana, Manuel R; Newman, W. H.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

Leeper-Woodfonl, S. K. ; Zhane, L. M. ; Castresana, Manuel R ; Newman, W. H. / Cyclic amp enhances toxicity of tumor necrosis factor alpha (tnf-α) in l929 cells. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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