Cyclooxygenase-2 preserves flow-mediated remodelling in old obese zucker rat mesenteric arteries

Eric Jacques Belin de Chantemele, Emilie Vessires, Anne Laure Guihot, Bertrand Toutain, Laurent Loufrani, Daniel Henrion

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

AimsResistance arteries have a key role in the control of local blood flow and pressure, and chronic increases in blood flow induce endothelium-dependent outward hypertrophic remodelling. The incidence of metabolic syndrome increases with age, and the combination of these two risk factors impairs endothelium integrity, in part through an inflammatory process. We hypothesized that cyclooxygenase-2 (COX2) would affect remodelling in 12-month-old obese rats compared with young rats.Methods and resultsMesenteric arteries of obese and lean Zucker rats were alternatively ligated to generate high flow (HF) in the median artery. After 21 days, arteries were isolated for in vitro analysis. After 21 days, outward hypertrophic remodelling occurred in HF arteries in obese (498 ± 20 vs. 443 ± 18 m intraluminal diameter in normal flow (NF) arteries, P < 0.01), but not in lean rats (454 ± 17 vs. 432 ± 14, NS; n = 12 per group). Endothelium-dependent (acetylcholine)- mediated relaxation (AMR) was lower in obese than in lean rats. AMR was reduced by NO-synthase blockade in all groups, and eNOS expression was higher in HF than in NF arteries without difference between lean and obese rats. Indomethacin further reduced AMR in HF arteries from obese rats only. Obesity increased COX2 immunostaining in mesenteric arteries. Acute COX2 inhibition (NS398) significantly reduced AMR in HF arteries from obese rats only, suggesting production of vasodilator prostanoid(s). In obese rats chronically treated with the COX2 inhibitor celecoxib, outward remodelling did not occur in HF arteries and AMR was improved without reaching the level found in lean rats. Conclusion COX2 preserved in part flow-mediated arterial remodelling in old obese rats. Nevertheless, this effect was not sufficient to keep endothelium-dependent relaxation to the level obtained in lean rats.

Original languageEnglish (US)
Pages (from-to)516-525
Number of pages10
JournalCardiovascular Research
Volume86
Issue number3
DOIs
StatePublished - Jun 1 2010

Fingerprint

Zucker Rats
Mesenteric Arteries
Cyclooxygenase 2
Arteries
Acetylcholine
Endothelium
Celecoxib
Cyclooxygenase 2 Inhibitors
Vasodilator Agents
Nitric Oxide Synthase
Indomethacin
Prostaglandins
Obesity

Keywords

  • Ageing
  • Cyclooxygenase-2
  • Metabolic syndrome
  • Resistance arteries
  • Shear stress

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Cyclooxygenase-2 preserves flow-mediated remodelling in old obese zucker rat mesenteric arteries. / Belin de Chantemele, Eric Jacques; Vessires, Emilie; Guihot, Anne Laure; Toutain, Bertrand; Loufrani, Laurent; Henrion, Daniel.

In: Cardiovascular Research, Vol. 86, No. 3, 01.06.2010, p. 516-525.

Research output: Contribution to journalArticle

Belin de Chantemele, Eric Jacques ; Vessires, Emilie ; Guihot, Anne Laure ; Toutain, Bertrand ; Loufrani, Laurent ; Henrion, Daniel. / Cyclooxygenase-2 preserves flow-mediated remodelling in old obese zucker rat mesenteric arteries. In: Cardiovascular Research. 2010 ; Vol. 86, No. 3. pp. 516-525.
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AU - Belin de Chantemele, Eric Jacques

AU - Vessires, Emilie

AU - Guihot, Anne Laure

AU - Toutain, Bertrand

AU - Loufrani, Laurent

AU - Henrion, Daniel

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KW - Ageing

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KW - Resistance arteries

KW - Shear stress

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