Cysteine cathepsins in human carious dentin

F. D. Nascimento, C. L. Minciotti, S. Geraldeli, M. R. Carrilho, David Henry Pashley, Franklin Chi Meng Tay, H. B. Nader, T. Salo, L. Tjäderhane, I. L.S. Tersariol

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Matrix metalloproteinases (MMPs) are important in dentinal caries, and analysis of recent data demonstrates the presence of other collagen-degrading enzymes, cysteine cathepsins, in human dentin. This study aimed to examine the presence, source, and activity of cysteine cathepsins in human caries. Cathepsin B was detected with immunostaining. Saliva and dentin cysteine cathepsin and MMP activities on caries lesions were analyzed spectrofluorometrically. Immunostaining demonstrated stronger cathepsins B in carious than in healthy dentin. In carious dentin, cysteine cathepsin activity increased with increasing depth and age in chronic lesions, but decreased with age in active lesions. MMP activity decreased with age in both active and chronic lesions. Salivary MMP activities were higher in patients with active than chronic lesions and with increasing lesion depth, while cysteine cathepsin activities showed no differences. The results indicate that, along with MMPs, cysteine cathepsins are important, especially in active and deep caries.

Original languageEnglish (US)
Pages (from-to)506-511
Number of pages6
JournalJournal of Dental Research
Volume90
Issue number4
DOIs
StatePublished - Apr 1 2011

Fingerprint

Cathepsins
Dental Caries
Cysteine
Matrix Metalloproteinases
Dentin
Cathepsin B
Collagenases
Saliva

Keywords

  • MMP
  • cathepsin
  • dentin
  • enzyme
  • human
  • tooth

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Nascimento, F. D., Minciotti, C. L., Geraldeli, S., Carrilho, M. R., Pashley, D. H., Tay, F. C. M., ... Tersariol, I. L. S. (2011). Cysteine cathepsins in human carious dentin. Journal of Dental Research, 90(4), 506-511. https://doi.org/10.1177/0022034510391906

Cysteine cathepsins in human carious dentin. / Nascimento, F. D.; Minciotti, C. L.; Geraldeli, S.; Carrilho, M. R.; Pashley, David Henry; Tay, Franklin Chi Meng; Nader, H. B.; Salo, T.; Tjäderhane, L.; Tersariol, I. L.S.

In: Journal of Dental Research, Vol. 90, No. 4, 01.04.2011, p. 506-511.

Research output: Contribution to journalArticle

Nascimento, FD, Minciotti, CL, Geraldeli, S, Carrilho, MR, Pashley, DH, Tay, FCM, Nader, HB, Salo, T, Tjäderhane, L & Tersariol, ILS 2011, 'Cysteine cathepsins in human carious dentin', Journal of Dental Research, vol. 90, no. 4, pp. 506-511. https://doi.org/10.1177/0022034510391906
Nascimento FD, Minciotti CL, Geraldeli S, Carrilho MR, Pashley DH, Tay FCM et al. Cysteine cathepsins in human carious dentin. Journal of Dental Research. 2011 Apr 1;90(4):506-511. https://doi.org/10.1177/0022034510391906
Nascimento, F. D. ; Minciotti, C. L. ; Geraldeli, S. ; Carrilho, M. R. ; Pashley, David Henry ; Tay, Franklin Chi Meng ; Nader, H. B. ; Salo, T. ; Tjäderhane, L. ; Tersariol, I. L.S. / Cysteine cathepsins in human carious dentin. In: Journal of Dental Research. 2011 ; Vol. 90, No. 4. pp. 506-511.
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