Cysteine Cathepsins in Human Dentin-Pulp Complex

Ivarne L. Tersariol, Saulo Geraldeli, Christiane L. Minciotti, Fábio D. Nascimento, Virve Pääkkönen, Marília T. Martins, Marcela R. Carrilho, David H. Pashley, Franklin Chi Meng Tay, Tuula Salo, Leo Tjäderhane

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Introduction: Collagen-degrading matrix metalloproteinases (MMPs) are expressed by odontoblasts and present in dentin. We hypothesized that odontoblasts express other collagen-degrading enzymes such as cysteine cathepsins, and their activity would be present in dentin, because odontoblasts are known to express at least cathepsin D. Effect of transforming growth factor beta (TGF-β) on cathepsin expression was also analyzed. Methods: Human odontoblasts and pulp tissue were cultured with and without TGF-β, and cathepsin gene expression was analyzed with DNA microarrays. Dentin cathepsin and MMP activities were analyzed by degradation of respective specific fluorogenic substrates. Results: Both odontoblasts and pulp tissue demonstrated a wide range of cysteine cathepsin expression that gave minor responses to TGF-β. Cathepsin and MMP activities were observed in all dentin samples, with significant negative correlations in their activities with tooth age. Conclusions: These results demonstrate for the first time the presence of cysteine cathepsins in dentin and suggest their role, along with MMPs, in dentin modification with aging.

Original languageEnglish (US)
Pages (from-to)475-481
Number of pages7
JournalJournal of Endodontics
Volume36
Issue number3
DOIs
StatePublished - Mar 1 2010

Fingerprint

Cathepsins
Dentin
Odontoblasts
Cysteine
Matrix Metalloproteinases
Transforming Growth Factor beta
Cathepsin D
Collagenases
Oligonucleotide Array Sequence Analysis
Fluorescent Dyes
Tooth
Collagen
Gene Expression

Keywords

  • Enzyme
  • human
  • MMP
  • odontoblast
  • tooth

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Tersariol, I. L., Geraldeli, S., Minciotti, C. L., Nascimento, F. D., Pääkkönen, V., Martins, M. T., ... Tjäderhane, L. (2010). Cysteine Cathepsins in Human Dentin-Pulp Complex. Journal of Endodontics, 36(3), 475-481. https://doi.org/10.1016/j.joen.2009.12.034

Cysteine Cathepsins in Human Dentin-Pulp Complex. / Tersariol, Ivarne L.; Geraldeli, Saulo; Minciotti, Christiane L.; Nascimento, Fábio D.; Pääkkönen, Virve; Martins, Marília T.; Carrilho, Marcela R.; Pashley, David H.; Tay, Franklin Chi Meng; Salo, Tuula; Tjäderhane, Leo.

In: Journal of Endodontics, Vol. 36, No. 3, 01.03.2010, p. 475-481.

Research output: Contribution to journalArticle

Tersariol, IL, Geraldeli, S, Minciotti, CL, Nascimento, FD, Pääkkönen, V, Martins, MT, Carrilho, MR, Pashley, DH, Tay, FCM, Salo, T & Tjäderhane, L 2010, 'Cysteine Cathepsins in Human Dentin-Pulp Complex', Journal of Endodontics, vol. 36, no. 3, pp. 475-481. https://doi.org/10.1016/j.joen.2009.12.034
Tersariol IL, Geraldeli S, Minciotti CL, Nascimento FD, Pääkkönen V, Martins MT et al. Cysteine Cathepsins in Human Dentin-Pulp Complex. Journal of Endodontics. 2010 Mar 1;36(3):475-481. https://doi.org/10.1016/j.joen.2009.12.034
Tersariol, Ivarne L. ; Geraldeli, Saulo ; Minciotti, Christiane L. ; Nascimento, Fábio D. ; Pääkkönen, Virve ; Martins, Marília T. ; Carrilho, Marcela R. ; Pashley, David H. ; Tay, Franklin Chi Meng ; Salo, Tuula ; Tjäderhane, Leo. / Cysteine Cathepsins in Human Dentin-Pulp Complex. In: Journal of Endodontics. 2010 ; Vol. 36, No. 3. pp. 475-481.
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