Cysteine proteases from the Asclepiadaceae plants latex exhibited thrombin and plasmin like activities

H. V. Shivaprasad, M. Riyaz, R. Venkatesh Kumar, K. K. Dharmappa, Shaista Tarannum, J. M. Siddesha, R. Rajesh, B. S. Vishwanath

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In the present study we evaluated the presence of cysteine protease from the latex of four plants Asclepias curassavica L., Calotropis gigantea R.Br., Pergularia extensa R.Br. and Cynanchum puciflorum R.Br. belongs to the family Asclepiadaceae. Cysteine proteases from these plants latex exhibited both thrombin and plasmin like activities. Latex enzyme fraction in a concentration dependent manner induced the formation of clot in citrated blood plasma. Direct incubation of fibrinogen with latex enzyme fraction resulted in the formation of fibrin clot similar to thrombin enzyme. However prolonged incubation resulted in degradation of the formed fibrin clot suggesting plasmin like activity. Latex enzyme fraction preferentially hydrolyzed Aα and Bβ chains of fibrinogen to form fibrin clot. Latex enzyme fraction also hydrolyzed the subunits of fully cross linked fibrin efficiently, the order of hydrolysis was α-polymer > α-chains > β-chain and γ-γ dimer. Cysteine proteases from all the four Asclepiadaceae plants latex exhibited similar action on fibrinogen and fibrin. This study scientifically validate the use of plant latex in stop bleeding and wound healing by traditional healers all over the world.

Original languageEnglish (US)
Pages (from-to)304-308
Number of pages5
JournalJournal of Thrombosis and Thrombolysis
Volume28
Issue number3
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Fingerprint

Apocynaceae
Cysteine Proteases
Fibrinolysin
Latex
Thrombin
Fibrin
Fibrinogen
Enzymes
Cynanchum
Asclepias
Calotropis
Wound Healing
Polymers
Hydrolysis
Hemorrhage

Keywords

  • Asclepiadaceae
  • Cysteine proteases
  • Plasmin like enzymes
  • Thrombin like enzymes

ASJC Scopus subject areas

  • Hematology
  • Cardiology and Cardiovascular Medicine

Cite this

Shivaprasad, H. V., Riyaz, M., Venkatesh Kumar, R., Dharmappa, K. K., Tarannum, S., Siddesha, J. M., ... Vishwanath, B. S. (2009). Cysteine proteases from the Asclepiadaceae plants latex exhibited thrombin and plasmin like activities. Journal of Thrombosis and Thrombolysis, 28(3), 304-308. https://doi.org/10.1007/s11239-008-0290-2

Cysteine proteases from the Asclepiadaceae plants latex exhibited thrombin and plasmin like activities. / Shivaprasad, H. V.; Riyaz, M.; Venkatesh Kumar, R.; Dharmappa, K. K.; Tarannum, Shaista; Siddesha, J. M.; Rajesh, R.; Vishwanath, B. S.

In: Journal of Thrombosis and Thrombolysis, Vol. 28, No. 3, 01.01.2009, p. 304-308.

Research output: Contribution to journalArticle

Shivaprasad, HV, Riyaz, M, Venkatesh Kumar, R, Dharmappa, KK, Tarannum, S, Siddesha, JM, Rajesh, R & Vishwanath, BS 2009, 'Cysteine proteases from the Asclepiadaceae plants latex exhibited thrombin and plasmin like activities', Journal of Thrombosis and Thrombolysis, vol. 28, no. 3, pp. 304-308. https://doi.org/10.1007/s11239-008-0290-2
Shivaprasad, H. V. ; Riyaz, M. ; Venkatesh Kumar, R. ; Dharmappa, K. K. ; Tarannum, Shaista ; Siddesha, J. M. ; Rajesh, R. ; Vishwanath, B. S. / Cysteine proteases from the Asclepiadaceae plants latex exhibited thrombin and plasmin like activities. In: Journal of Thrombosis and Thrombolysis. 2009 ; Vol. 28, No. 3. pp. 304-308.
@article{017827ef8c194a9b80eac3eed82a3999,
title = "Cysteine proteases from the Asclepiadaceae plants latex exhibited thrombin and plasmin like activities",
abstract = "In the present study we evaluated the presence of cysteine protease from the latex of four plants Asclepias curassavica L., Calotropis gigantea R.Br., Pergularia extensa R.Br. and Cynanchum puciflorum R.Br. belongs to the family Asclepiadaceae. Cysteine proteases from these plants latex exhibited both thrombin and plasmin like activities. Latex enzyme fraction in a concentration dependent manner induced the formation of clot in citrated blood plasma. Direct incubation of fibrinogen with latex enzyme fraction resulted in the formation of fibrin clot similar to thrombin enzyme. However prolonged incubation resulted in degradation of the formed fibrin clot suggesting plasmin like activity. Latex enzyme fraction preferentially hydrolyzed Aα and Bβ chains of fibrinogen to form fibrin clot. Latex enzyme fraction also hydrolyzed the subunits of fully cross linked fibrin efficiently, the order of hydrolysis was α-polymer > α-chains > β-chain and γ-γ dimer. Cysteine proteases from all the four Asclepiadaceae plants latex exhibited similar action on fibrinogen and fibrin. This study scientifically validate the use of plant latex in stop bleeding and wound healing by traditional healers all over the world.",
keywords = "Asclepiadaceae, Cysteine proteases, Plasmin like enzymes, Thrombin like enzymes",
author = "Shivaprasad, {H. V.} and M. Riyaz and {Venkatesh Kumar}, R. and Dharmappa, {K. K.} and Shaista Tarannum and Siddesha, {J. M.} and R. Rajesh and Vishwanath, {B. S.}",
year = "2009",
month = "1",
day = "1",
doi = "10.1007/s11239-008-0290-2",
language = "English (US)",
volume = "28",
pages = "304--308",
journal = "Journal of Thrombosis and Thrombolysis",
issn = "0929-5305",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Cysteine proteases from the Asclepiadaceae plants latex exhibited thrombin and plasmin like activities

AU - Shivaprasad, H. V.

AU - Riyaz, M.

AU - Venkatesh Kumar, R.

AU - Dharmappa, K. K.

AU - Tarannum, Shaista

AU - Siddesha, J. M.

AU - Rajesh, R.

AU - Vishwanath, B. S.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - In the present study we evaluated the presence of cysteine protease from the latex of four plants Asclepias curassavica L., Calotropis gigantea R.Br., Pergularia extensa R.Br. and Cynanchum puciflorum R.Br. belongs to the family Asclepiadaceae. Cysteine proteases from these plants latex exhibited both thrombin and plasmin like activities. Latex enzyme fraction in a concentration dependent manner induced the formation of clot in citrated blood plasma. Direct incubation of fibrinogen with latex enzyme fraction resulted in the formation of fibrin clot similar to thrombin enzyme. However prolonged incubation resulted in degradation of the formed fibrin clot suggesting plasmin like activity. Latex enzyme fraction preferentially hydrolyzed Aα and Bβ chains of fibrinogen to form fibrin clot. Latex enzyme fraction also hydrolyzed the subunits of fully cross linked fibrin efficiently, the order of hydrolysis was α-polymer > α-chains > β-chain and γ-γ dimer. Cysteine proteases from all the four Asclepiadaceae plants latex exhibited similar action on fibrinogen and fibrin. This study scientifically validate the use of plant latex in stop bleeding and wound healing by traditional healers all over the world.

AB - In the present study we evaluated the presence of cysteine protease from the latex of four plants Asclepias curassavica L., Calotropis gigantea R.Br., Pergularia extensa R.Br. and Cynanchum puciflorum R.Br. belongs to the family Asclepiadaceae. Cysteine proteases from these plants latex exhibited both thrombin and plasmin like activities. Latex enzyme fraction in a concentration dependent manner induced the formation of clot in citrated blood plasma. Direct incubation of fibrinogen with latex enzyme fraction resulted in the formation of fibrin clot similar to thrombin enzyme. However prolonged incubation resulted in degradation of the formed fibrin clot suggesting plasmin like activity. Latex enzyme fraction preferentially hydrolyzed Aα and Bβ chains of fibrinogen to form fibrin clot. Latex enzyme fraction also hydrolyzed the subunits of fully cross linked fibrin efficiently, the order of hydrolysis was α-polymer > α-chains > β-chain and γ-γ dimer. Cysteine proteases from all the four Asclepiadaceae plants latex exhibited similar action on fibrinogen and fibrin. This study scientifically validate the use of plant latex in stop bleeding and wound healing by traditional healers all over the world.

KW - Asclepiadaceae

KW - Cysteine proteases

KW - Plasmin like enzymes

KW - Thrombin like enzymes

UR - http://www.scopus.com/inward/record.url?scp=70349098140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349098140&partnerID=8YFLogxK

U2 - 10.1007/s11239-008-0290-2

DO - 10.1007/s11239-008-0290-2

M3 - Article

C2 - 18979066

AN - SCOPUS:70349098140

VL - 28

SP - 304

EP - 308

JO - Journal of Thrombosis and Thrombolysis

JF - Journal of Thrombosis and Thrombolysis

SN - 0929-5305

IS - 3

ER -