TY - JOUR
T1 - Cytochalasin selectively inhibits synthesis of a secretory protein, cellulose, in Achlya
AU - Thomas, Donovan Des S.
AU - Lutzac, Marjorie
AU - Manavathu, Elias
PY - 1974
Y1 - 1974
N2 - IN an effort to elucidate the possible relationship between cytoplasmic streaming and secretion we used the male strain E87 of the water mould Achlya ambisexualis1. Cellulase secreted by Achlya presumably modifies the plasticity of the cellulosic walls of this fungus during morphogenesis 2. Synthesis of this enzyme can be induced by the steroid hormone antheridiol, by amino acids2, or by shake induction (mechanical agitation) as reported here. In attempts to arrest streaming we used colchicine and vinblastine, which have been employed to disrupt microtubule function, and cytochalasins, whose reversible effects include inhibition of streaming 3, of macromolecular secretion4, of uptake of small molecules such as glucose5, and destruction of microfilaments 6.
AB - IN an effort to elucidate the possible relationship between cytoplasmic streaming and secretion we used the male strain E87 of the water mould Achlya ambisexualis1. Cellulase secreted by Achlya presumably modifies the plasticity of the cellulosic walls of this fungus during morphogenesis 2. Synthesis of this enzyme can be induced by the steroid hormone antheridiol, by amino acids2, or by shake induction (mechanical agitation) as reported here. In attempts to arrest streaming we used colchicine and vinblastine, which have been employed to disrupt microtubule function, and cytochalasins, whose reversible effects include inhibition of streaming 3, of macromolecular secretion4, of uptake of small molecules such as glucose5, and destruction of microfilaments 6.
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U2 - 10.1038/249140a0
DO - 10.1038/249140a0
M3 - Article
C2 - 4833510
AN - SCOPUS:0016351196
SN - 0028-0836
VL - 249
SP - 140
EP - 142
JO - Nature
JF - Nature
IS - 5453
ER -