Cytochrome c oxidase immobilized in stable supported lipid bilayer membranes

James D. Burgess, Melissa C. Rhoten, Fred M. Hawkridge

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

The voltammetry of cytochrome c oxidase immobilized in lipid bilayer membranes on gold electrodes and amperometric data of cytochrome c reacting at these electrodes under flow conditions are reported. A submonolayer of octadecyl mercaptan formed on electrodeposited silver anchors and becomes a part of the lipid bilayer membrane on the gold electrode. The supported lipid bilayer membrane containing cytochrome c oxidase is formed during a deoxycholate dialysis procedure. Slow scan rate cyclic voltammograms (20 mV/s) taken at the oxidase-modified electrodes show well-defined anodic waves. Fast scan rate cyclic voltammograms (200 mV/s) taken at the oxidase-modified electrodes show well-defined anodic and cathodic waves. Cyclic voltammograms taken at the oxidase-modified electrodes under 0.1 mM sodium cyanide show an increase (ca. 300%) in electrode capacitance and well-defined anodic and cathodic waves irrespective of scan rate. The voltammetric data are consistent with electron transfer of cytochrome c oxidase coupled with changes in nonfaradaic current and possibly diffusion of cytochrome c oxidase in a lipid multilayer structure. Quartz crystal microbalance data of cytochrome c binding to lipid bilayer membranes containing no cytochrome c oxidase under flow conditions are presented.

Original languageEnglish (US)
Pages (from-to)2467-2475
Number of pages9
JournalLangmuir
Volume14
Issue number9
DOIs
StatePublished - Apr 28 1998

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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    Burgess, J. D., Rhoten, M. C., & Hawkridge, F. M. (1998). Cytochrome c oxidase immobilized in stable supported lipid bilayer membranes. Langmuir, 14(9), 2467-2475. https://doi.org/10.1021/la9711995