Cytochrome P450(11 β): Ectopic expression in oocyte and its physiological implication

Mitsuhiro Okamoto, Hiroshi Takemori, Sunil K. Halder, Yasuki Nonaka

Research output: Contribution to journalArticle

Abstract

Cytochrome P450(11 β) (steroid 11 β-hydroxylase) catalyzes an essential step for biosynthesis of glucocorticoid as well as mineralocorticoid, and therefore is believed to exist specifically in the adrenocortical mitochondria. We previously isolated a complementary DNA clone encoding this enzyme from bullfrog (Rana catesbeiana) adrenal tissues and characterized the enzyme expressed in COS cells. To explore the tissue distribution of the enzyme we performed Northern blot analysis. Unexpectedly we found that a large amount of the mRNA exists in the ovary as well as the adrenal gland. It was present in oocytes, but not in follicular cells. The P450 protein was not found during early stages of oocyte development, and at the final stage of maturation the message appeared to be translated. Evidence was obtained that the stage-dependent translation of the mRNA was due to the regulated polyadenylation at its 3′-end. In oocytes of various organisms several maternal mRNAs are known to add poly(A) tails in the cytoplasm during the progesterone-induced maturation.

Original languageEnglish (US)
Pages (from-to)471-477
Number of pages7
JournalEndocrine Research
Volume22
Issue number4
DOIs
StatePublished - Jan 1 1996

Fingerprint

Cytochrome P-450 Enzyme System
Oocytes
Rana catesbeiana
Enzymes
Steroid 11-beta-Hydroxylase
Stored Messenger RNA
Mineralocorticoids
Messenger RNA
Polyadenylation
COS Cells
Protein Biosynthesis
Tissue Distribution
Adrenal Glands
Northern Blotting
Glucocorticoids
Progesterone
Ovary
Mitochondria
Cytoplasm
Complementary DNA

ASJC Scopus subject areas

  • Endocrinology

Cite this

Cytochrome P450(11 β) : Ectopic expression in oocyte and its physiological implication. / Okamoto, Mitsuhiro; Takemori, Hiroshi; Halder, Sunil K.; Nonaka, Yasuki.

In: Endocrine Research, Vol. 22, No. 4, 01.01.1996, p. 471-477.

Research output: Contribution to journalArticle

Okamoto, Mitsuhiro ; Takemori, Hiroshi ; Halder, Sunil K. ; Nonaka, Yasuki. / Cytochrome P450(11 β) : Ectopic expression in oocyte and its physiological implication. In: Endocrine Research. 1996 ; Vol. 22, No. 4. pp. 471-477.
@article{394f6d0e9b2f4471b49f3c2dcbc01eec,
title = "Cytochrome P450(11 β): Ectopic expression in oocyte and its physiological implication",
abstract = "Cytochrome P450(11 β) (steroid 11 β-hydroxylase) catalyzes an essential step for biosynthesis of glucocorticoid as well as mineralocorticoid, and therefore is believed to exist specifically in the adrenocortical mitochondria. We previously isolated a complementary DNA clone encoding this enzyme from bullfrog (Rana catesbeiana) adrenal tissues and characterized the enzyme expressed in COS cells. To explore the tissue distribution of the enzyme we performed Northern blot analysis. Unexpectedly we found that a large amount of the mRNA exists in the ovary as well as the adrenal gland. It was present in oocytes, but not in follicular cells. The P450 protein was not found during early stages of oocyte development, and at the final stage of maturation the message appeared to be translated. Evidence was obtained that the stage-dependent translation of the mRNA was due to the regulated polyadenylation at its 3′-end. In oocytes of various organisms several maternal mRNAs are known to add poly(A) tails in the cytoplasm during the progesterone-induced maturation.",
author = "Mitsuhiro Okamoto and Hiroshi Takemori and Halder, {Sunil K.} and Yasuki Nonaka",
year = "1996",
month = "1",
day = "1",
doi = "10.1080/07435809609043733",
language = "English (US)",
volume = "22",
pages = "471--477",
journal = "Endocrine Research",
issn = "0743-5800",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - Cytochrome P450(11 β)

T2 - Ectopic expression in oocyte and its physiological implication

AU - Okamoto, Mitsuhiro

AU - Takemori, Hiroshi

AU - Halder, Sunil K.

AU - Nonaka, Yasuki

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Cytochrome P450(11 β) (steroid 11 β-hydroxylase) catalyzes an essential step for biosynthesis of glucocorticoid as well as mineralocorticoid, and therefore is believed to exist specifically in the adrenocortical mitochondria. We previously isolated a complementary DNA clone encoding this enzyme from bullfrog (Rana catesbeiana) adrenal tissues and characterized the enzyme expressed in COS cells. To explore the tissue distribution of the enzyme we performed Northern blot analysis. Unexpectedly we found that a large amount of the mRNA exists in the ovary as well as the adrenal gland. It was present in oocytes, but not in follicular cells. The P450 protein was not found during early stages of oocyte development, and at the final stage of maturation the message appeared to be translated. Evidence was obtained that the stage-dependent translation of the mRNA was due to the regulated polyadenylation at its 3′-end. In oocytes of various organisms several maternal mRNAs are known to add poly(A) tails in the cytoplasm during the progesterone-induced maturation.

AB - Cytochrome P450(11 β) (steroid 11 β-hydroxylase) catalyzes an essential step for biosynthesis of glucocorticoid as well as mineralocorticoid, and therefore is believed to exist specifically in the adrenocortical mitochondria. We previously isolated a complementary DNA clone encoding this enzyme from bullfrog (Rana catesbeiana) adrenal tissues and characterized the enzyme expressed in COS cells. To explore the tissue distribution of the enzyme we performed Northern blot analysis. Unexpectedly we found that a large amount of the mRNA exists in the ovary as well as the adrenal gland. It was present in oocytes, but not in follicular cells. The P450 protein was not found during early stages of oocyte development, and at the final stage of maturation the message appeared to be translated. Evidence was obtained that the stage-dependent translation of the mRNA was due to the regulated polyadenylation at its 3′-end. In oocytes of various organisms several maternal mRNAs are known to add poly(A) tails in the cytoplasm during the progesterone-induced maturation.

UR - http://www.scopus.com/inward/record.url?scp=0030474849&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030474849&partnerID=8YFLogxK

U2 - 10.1080/07435809609043733

DO - 10.1080/07435809609043733

M3 - Article

C2 - 8969898

AN - SCOPUS:0030474849

VL - 22

SP - 471

EP - 477

JO - Endocrine Research

JF - Endocrine Research

SN - 0743-5800

IS - 4

ER -