Cytotoxic effects of NaOCl on vital tissue

E. L. Pashley, N. L. Birdsong, K. Bowman, D. H. Pashley

Research output: Contribution to journalArticlepeer-review

185 Scopus citations


The cytotoxicity of various dilutions of sodium hypochlorite was evaluated using three independent biological models. Dilutions as low as 1:1000 caused complete hemolysis of RBC's in vitro. Undiluted and 1:10 dilutions produced moderate to severe irritation to rabbit eyes which healed after 24 to 48 h. Intradermal injections of undiluted, 1:1, 1:2, and 1:4 dilutions of NaOCl produced skin ulcerations following intradermal injections. Although the use of 5% NaOCl for biomechanical preparation of root canals is a clinically acceptable and highly effective procedure, it must be used judiciously and with great caution to prevent it from reaching the periapex where it can elicit severe inflammatory reactions.

Original languageEnglish (US)
Pages (from-to)525-528
Number of pages4
JournalJournal of endodontics
Issue number12
StatePublished - Dec 1985

ASJC Scopus subject areas

  • Dentistry(all)


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