Cytotoxic effects of suramin against HepG2 cells through activation of intrinsic apoptotic pathway

Ahmed Tayel, Mohamed A. Ebrahim, Ahmed Salah Ibrahim, Amal M. El-Gayar, Mohammed M. Al-Gayyar

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: To investigate the cytotoxic activity of suramin against HepG2 human HCC cell line. Methods: HepG2 cells were treated with 15, 30 and 45 uM suramin. HepG2 cell proliferation was measured by MTT and lactate dehydrogenase (LDH) assays. Heparan sulfate proteoglycans (HSPGs), glucuronic acid and glucosamine levels were measured. Moreover, apoptosis was assessed by measuring caspase-8 and caspase-9 activities. The effect of suramin on HepG2 cells was compared with the same doses of a standard drug, cisplatin. Results: Suramin blocked heparanase leading to dose-dependent increase in HSPGs and reduction in glucuronic acid and glucosamine levels. Suramin reduced HepG2 cells survival and showed cell cytotoxicity in a dose-dependent manner with LD50 45.04 uM, compared with cisplatin (LD50 28.9 uM) (p<0.05). Moreover, suramin was able to increase the activity of caspase-9 but not of caspase-8. Conclusion: Suramin possesses cytotoxic properties, which can be partially explained by its ability to inhibit heparanase and restore HSPGs. Suramin activates intrinsic apoptosis without affecting the extrinsic pathway.

Original languageEnglish (US)
Pages (from-to)1048-1054
Number of pages7
JournalJournal of B.U.ON.
Volume19
Issue number4
StatePublished - Oct 1 2014
Externally publishedYes

Fingerprint

Suramin
Hep G2 Cells
Heparan Sulfate Proteoglycans
Glucuronic Acid
Caspase 9
Caspase 8
Lethal Dose 50
Glucosamine
Cisplatin
Apoptosis
L-Lactate Dehydrogenase
Cell Survival
Cell Proliferation
Cell Line

Keywords

  • Caspase-8
  • Caspase-9
  • Glucosamine
  • Glucuronic acid
  • Heparan sulfate proteoglycans
  • Heparanase

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Tayel, A., Ebrahim, M. A., Ibrahim, A. S., El-Gayar, A. M., & Al-Gayyar, M. M. (2014). Cytotoxic effects of suramin against HepG2 cells through activation of intrinsic apoptotic pathway. Journal of B.U.ON., 19(4), 1048-1054.

Cytotoxic effects of suramin against HepG2 cells through activation of intrinsic apoptotic pathway. / Tayel, Ahmed; Ebrahim, Mohamed A.; Ibrahim, Ahmed Salah; El-Gayar, Amal M.; Al-Gayyar, Mohammed M.

In: Journal of B.U.ON., Vol. 19, No. 4, 01.10.2014, p. 1048-1054.

Research output: Contribution to journalArticle

Tayel, A, Ebrahim, MA, Ibrahim, AS, El-Gayar, AM & Al-Gayyar, MM 2014, 'Cytotoxic effects of suramin against HepG2 cells through activation of intrinsic apoptotic pathway', Journal of B.U.ON., vol. 19, no. 4, pp. 1048-1054.
Tayel A, Ebrahim MA, Ibrahim AS, El-Gayar AM, Al-Gayyar MM. Cytotoxic effects of suramin against HepG2 cells through activation of intrinsic apoptotic pathway. Journal of B.U.ON. 2014 Oct 1;19(4):1048-1054.
Tayel, Ahmed ; Ebrahim, Mohamed A. ; Ibrahim, Ahmed Salah ; El-Gayar, Amal M. ; Al-Gayyar, Mohammed M. / Cytotoxic effects of suramin against HepG2 cells through activation of intrinsic apoptotic pathway. In: Journal of B.U.ON. 2014 ; Vol. 19, No. 4. pp. 1048-1054.
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