Cytotoxicity, calcium release, and pH changes generated by novel calcium phosphate cement formulations

Rania M. Khashaba, Petra E. Lockwood, Jill B. Lewis, Regina L W Messer, Norman B. Chutkan, James L. Borke

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Few published studies describe the biological properties of calcium phosphate cements (CPCs) for dental applications. We measured several biologically relevant properties of 3 CPCs over an extended (8 wk) interval. Monocalcium phosphate, calcium oxide, and synthetic hydroxyapatite were combined with either modified polyacrylic acid, light-activated modified polyalkenoic acid, or 35% w/w polymethyl vinyl ether maleic acid to obtain Types I, II, and III CPCs, respectively. Set cements were placed in direct contact with L929 fibroblasts for up to 8 weeks. Media Ca+2 and pH were determined by atomic absorption spectroscopy and pH electrode respectively. Cell mitochondrial function was measured by MTT assay. Type I cements suppressed mitochondrial activity > 90% (vs. Teflon controls), but significantly (p < 0.05) improved to control levels over 8 weeks. Type II cements suppressed mitochondrial activity > 90% at all times. Type III cements elevated mitochondrial activity significantly after 7 wks. The pH profiles approached neutrality by 24 h, and all cements released calcium into the storage medium at all periods (24 h - 8 wk). We concluded that several types of cements had long-term biological profiles that show promise for dental applications.

Original languageEnglish (US)
Pages (from-to)297-303
Number of pages7
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume93
Issue number2
DOIs
StatePublished - May 1 2010

Fingerprint

Calcium phosphate
Cytotoxicity
Calcium
Cements
carbopol 940
Dental Cements
Polytetrafluoroethylene
Durapatite
Spectrum Analysis
Tooth
Electrodes
Fibroblasts
Phosphates
Acids
Light
Atomic spectroscopy
calcium phosphate
Absorption spectroscopy
Hydroxyapatite
Polytetrafluoroethylenes

Keywords

  • Calcium phosphate(s)
  • Cell culture
  • Cytotoxicity
  • Dental/craniofacial material

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Cytotoxicity, calcium release, and pH changes generated by novel calcium phosphate cement formulations. / Khashaba, Rania M.; Lockwood, Petra E.; Lewis, Jill B.; Messer, Regina L W; Chutkan, Norman B.; Borke, James L.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 93, No. 2, 01.05.2010, p. 297-303.

Research output: Contribution to journalArticle

Khashaba, Rania M. ; Lockwood, Petra E. ; Lewis, Jill B. ; Messer, Regina L W ; Chutkan, Norman B. ; Borke, James L. / Cytotoxicity, calcium release, and pH changes generated by novel calcium phosphate cement formulations. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2010 ; Vol. 93, No. 2. pp. 297-303.
@article{ca4178c3635c48259b008ad5d5664802,
title = "Cytotoxicity, calcium release, and pH changes generated by novel calcium phosphate cement formulations",
abstract = "Few published studies describe the biological properties of calcium phosphate cements (CPCs) for dental applications. We measured several biologically relevant properties of 3 CPCs over an extended (8 wk) interval. Monocalcium phosphate, calcium oxide, and synthetic hydroxyapatite were combined with either modified polyacrylic acid, light-activated modified polyalkenoic acid, or 35{\%} w/w polymethyl vinyl ether maleic acid to obtain Types I, II, and III CPCs, respectively. Set cements were placed in direct contact with L929 fibroblasts for up to 8 weeks. Media Ca+2 and pH were determined by atomic absorption spectroscopy and pH electrode respectively. Cell mitochondrial function was measured by MTT assay. Type I cements suppressed mitochondrial activity > 90{\%} (vs. Teflon controls), but significantly (p < 0.05) improved to control levels over 8 weeks. Type II cements suppressed mitochondrial activity > 90{\%} at all times. Type III cements elevated mitochondrial activity significantly after 7 wks. The pH profiles approached neutrality by 24 h, and all cements released calcium into the storage medium at all periods (24 h - 8 wk). We concluded that several types of cements had long-term biological profiles that show promise for dental applications.",
keywords = "Calcium phosphate(s), Cell culture, Cytotoxicity, Dental/craniofacial material",
author = "Khashaba, {Rania M.} and Lockwood, {Petra E.} and Lewis, {Jill B.} and Messer, {Regina L W} and Chutkan, {Norman B.} and Borke, {James L.}",
year = "2010",
month = "5",
day = "1",
doi = "10.1002/jbm.b.31494",
language = "English (US)",
volume = "93",
pages = "297--303",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "0021-9304",
publisher = "Heterocorporation",
number = "2",

}

TY - JOUR

T1 - Cytotoxicity, calcium release, and pH changes generated by novel calcium phosphate cement formulations

AU - Khashaba, Rania M.

AU - Lockwood, Petra E.

AU - Lewis, Jill B.

AU - Messer, Regina L W

AU - Chutkan, Norman B.

AU - Borke, James L.

PY - 2010/5/1

Y1 - 2010/5/1

N2 - Few published studies describe the biological properties of calcium phosphate cements (CPCs) for dental applications. We measured several biologically relevant properties of 3 CPCs over an extended (8 wk) interval. Monocalcium phosphate, calcium oxide, and synthetic hydroxyapatite were combined with either modified polyacrylic acid, light-activated modified polyalkenoic acid, or 35% w/w polymethyl vinyl ether maleic acid to obtain Types I, II, and III CPCs, respectively. Set cements were placed in direct contact with L929 fibroblasts for up to 8 weeks. Media Ca+2 and pH were determined by atomic absorption spectroscopy and pH electrode respectively. Cell mitochondrial function was measured by MTT assay. Type I cements suppressed mitochondrial activity > 90% (vs. Teflon controls), but significantly (p < 0.05) improved to control levels over 8 weeks. Type II cements suppressed mitochondrial activity > 90% at all times. Type III cements elevated mitochondrial activity significantly after 7 wks. The pH profiles approached neutrality by 24 h, and all cements released calcium into the storage medium at all periods (24 h - 8 wk). We concluded that several types of cements had long-term biological profiles that show promise for dental applications.

AB - Few published studies describe the biological properties of calcium phosphate cements (CPCs) for dental applications. We measured several biologically relevant properties of 3 CPCs over an extended (8 wk) interval. Monocalcium phosphate, calcium oxide, and synthetic hydroxyapatite were combined with either modified polyacrylic acid, light-activated modified polyalkenoic acid, or 35% w/w polymethyl vinyl ether maleic acid to obtain Types I, II, and III CPCs, respectively. Set cements were placed in direct contact with L929 fibroblasts for up to 8 weeks. Media Ca+2 and pH were determined by atomic absorption spectroscopy and pH electrode respectively. Cell mitochondrial function was measured by MTT assay. Type I cements suppressed mitochondrial activity > 90% (vs. Teflon controls), but significantly (p < 0.05) improved to control levels over 8 weeks. Type II cements suppressed mitochondrial activity > 90% at all times. Type III cements elevated mitochondrial activity significantly after 7 wks. The pH profiles approached neutrality by 24 h, and all cements released calcium into the storage medium at all periods (24 h - 8 wk). We concluded that several types of cements had long-term biological profiles that show promise for dental applications.

KW - Calcium phosphate(s)

KW - Cell culture

KW - Cytotoxicity

KW - Dental/craniofacial material

UR - http://www.scopus.com/inward/record.url?scp=77951163162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951163162&partnerID=8YFLogxK

U2 - 10.1002/jbm.b.31494

DO - 10.1002/jbm.b.31494

M3 - Article

C2 - 20235188

AN - SCOPUS:77951163162

VL - 93

SP - 297

EP - 303

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 0021-9304

IS - 2

ER -