Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf

Mahendra Jain, Rakhee Kapadia, Ravirajsinh N. Jadeja, Menaka C. Thounaojam, Ranjitsinh V. Devkar, S. H. Mishra

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Objective: To evaluate the cytotoxicity and hepatoprotective potentials of extracts, fractions or isolated compound from the leaves of Feronia limonia (F. limonia). Methods: Qualitative phytochemical analysis of extracts, fractions or compound was performed by means of thin layer chromatography and spectroscopic assays. The % purity of compound was measured by analytical HPLC. Extracts, fractions or compound have been individually evaluated for their cytotoxicity effects (10, 20, 100, 250, 500, 750 and 1 000 μg/mL). Based on the inhibitory concentration (IC 50 ) obtained from the cell viability assay, graded concentrations of extracts, fractions or isolated compound were assessed (10, 20, 50, 100, 200 μg/mL) for its hepatoprotective potential against CCl 4 -induced hepatotoxicity by monitoring activity levels of serum glutamatic pyruvatic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT). Results: Results indicated that the methanol extract of F. limonia was non-toxic and hepatoprotective in nature as compared with the petroleum ether extract. The acetone fraction of methanolic extract also showed similar properties but the subsequent two fractions were cytotoxic. However, the pure compound isolated from the penultimate fraction of methanolic extract was non-toxic and hepatoprotective in nature. Biochemical investigations (SGOT, SGPT) further corroborated these cytological observations. Conclusions: It can be concluded from this study that F. limonia methanol extract, some fractions and pure isolated compound herein exhibit hepatoprotective activity. However, cytotoxicity recorded in the penultimate fraction and investigation of structural details of pure compound warrants further study.

Original languageEnglish (US)
Pages (from-to)443-447
Number of pages5
JournalAsian Pacific Journal of Tropical Biomedicine
Volume1
Issue number6
DOIs
StatePublished - Dec 2011
Externally publishedYes

Fingerprint

Plumbaginaceae
Bioassay
Cytotoxicity
Biological Assay
Aspartate Aminotransferases
Transaminases
Methanol
Assays
Thin layer chromatography
Phytochemicals
Thin Layer Chromatography
Acetone
Serum
Inhibitory Concentration 50
Cell Survival
High Pressure Liquid Chromatography
Cells
Monitoring

Keywords

  • Cytotoxicity
  • Feronia limonia leaf
  • HPLC
  • HepG2
  • Hepatoprotective
  • Hepatoprotective activity
  • Isolated compound
  • Phytochemical analysis
  • SGOT
  • SGPT
  • Spectroscopic assay
  • TLC

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Cite this

Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf. / Jain, Mahendra; Kapadia, Rakhee; Jadeja, Ravirajsinh N.; Thounaojam, Menaka C.; Devkar, Ranjitsinh V.; Mishra, S. H.

In: Asian Pacific Journal of Tropical Biomedicine, Vol. 1, No. 6, 12.2011, p. 443-447.

Research output: Contribution to journalArticle

Jain, Mahendra ; Kapadia, Rakhee ; Jadeja, Ravirajsinh N. ; Thounaojam, Menaka C. ; Devkar, Ranjitsinh V. ; Mishra, S. H. / Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf. In: Asian Pacific Journal of Tropical Biomedicine. 2011 ; Vol. 1, No. 6. pp. 443-447.
@article{4567bcf6a0ac4a92bfdb6cec15c0756a,
title = "Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf",
abstract = "Objective: To evaluate the cytotoxicity and hepatoprotective potentials of extracts, fractions or isolated compound from the leaves of Feronia limonia (F. limonia). Methods: Qualitative phytochemical analysis of extracts, fractions or compound was performed by means of thin layer chromatography and spectroscopic assays. The {\%} purity of compound was measured by analytical HPLC. Extracts, fractions or compound have been individually evaluated for their cytotoxicity effects (10, 20, 100, 250, 500, 750 and 1 000 μg/mL). Based on the inhibitory concentration (IC 50 ) obtained from the cell viability assay, graded concentrations of extracts, fractions or isolated compound were assessed (10, 20, 50, 100, 200 μg/mL) for its hepatoprotective potential against CCl 4 -induced hepatotoxicity by monitoring activity levels of serum glutamatic pyruvatic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT). Results: Results indicated that the methanol extract of F. limonia was non-toxic and hepatoprotective in nature as compared with the petroleum ether extract. The acetone fraction of methanolic extract also showed similar properties but the subsequent two fractions were cytotoxic. However, the pure compound isolated from the penultimate fraction of methanolic extract was non-toxic and hepatoprotective in nature. Biochemical investigations (SGOT, SGPT) further corroborated these cytological observations. Conclusions: It can be concluded from this study that F. limonia methanol extract, some fractions and pure isolated compound herein exhibit hepatoprotective activity. However, cytotoxicity recorded in the penultimate fraction and investigation of structural details of pure compound warrants further study.",
keywords = "Cytotoxicity, Feronia limonia leaf, HPLC, HepG2, Hepatoprotective, Hepatoprotective activity, Isolated compound, Phytochemical analysis, SGOT, SGPT, Spectroscopic assay, TLC",
author = "Mahendra Jain and Rakhee Kapadia and Jadeja, {Ravirajsinh N.} and Thounaojam, {Menaka C.} and Devkar, {Ranjitsinh V.} and Mishra, {S. H.}",
year = "2011",
month = "12",
doi = "10.1016/S2221-1691(11)60097-X",
language = "English (US)",
volume = "1",
pages = "443--447",
journal = "Asian Pacific Journal of Tropical Biomedicine",
issn = "2221-1691",
publisher = "Elsevier BV",
number = "6",

}

TY - JOUR

T1 - Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia limmonia Linn leaf

AU - Jain, Mahendra

AU - Kapadia, Rakhee

AU - Jadeja, Ravirajsinh N.

AU - Thounaojam, Menaka C.

AU - Devkar, Ranjitsinh V.

AU - Mishra, S. H.

PY - 2011/12

Y1 - 2011/12

N2 - Objective: To evaluate the cytotoxicity and hepatoprotective potentials of extracts, fractions or isolated compound from the leaves of Feronia limonia (F. limonia). Methods: Qualitative phytochemical analysis of extracts, fractions or compound was performed by means of thin layer chromatography and spectroscopic assays. The % purity of compound was measured by analytical HPLC. Extracts, fractions or compound have been individually evaluated for their cytotoxicity effects (10, 20, 100, 250, 500, 750 and 1 000 μg/mL). Based on the inhibitory concentration (IC 50 ) obtained from the cell viability assay, graded concentrations of extracts, fractions or isolated compound were assessed (10, 20, 50, 100, 200 μg/mL) for its hepatoprotective potential against CCl 4 -induced hepatotoxicity by monitoring activity levels of serum glutamatic pyruvatic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT). Results: Results indicated that the methanol extract of F. limonia was non-toxic and hepatoprotective in nature as compared with the petroleum ether extract. The acetone fraction of methanolic extract also showed similar properties but the subsequent two fractions were cytotoxic. However, the pure compound isolated from the penultimate fraction of methanolic extract was non-toxic and hepatoprotective in nature. Biochemical investigations (SGOT, SGPT) further corroborated these cytological observations. Conclusions: It can be concluded from this study that F. limonia methanol extract, some fractions and pure isolated compound herein exhibit hepatoprotective activity. However, cytotoxicity recorded in the penultimate fraction and investigation of structural details of pure compound warrants further study.

AB - Objective: To evaluate the cytotoxicity and hepatoprotective potentials of extracts, fractions or isolated compound from the leaves of Feronia limonia (F. limonia). Methods: Qualitative phytochemical analysis of extracts, fractions or compound was performed by means of thin layer chromatography and spectroscopic assays. The % purity of compound was measured by analytical HPLC. Extracts, fractions or compound have been individually evaluated for their cytotoxicity effects (10, 20, 100, 250, 500, 750 and 1 000 μg/mL). Based on the inhibitory concentration (IC 50 ) obtained from the cell viability assay, graded concentrations of extracts, fractions or isolated compound were assessed (10, 20, 50, 100, 200 μg/mL) for its hepatoprotective potential against CCl 4 -induced hepatotoxicity by monitoring activity levels of serum glutamatic pyruvatic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT). Results: Results indicated that the methanol extract of F. limonia was non-toxic and hepatoprotective in nature as compared with the petroleum ether extract. The acetone fraction of methanolic extract also showed similar properties but the subsequent two fractions were cytotoxic. However, the pure compound isolated from the penultimate fraction of methanolic extract was non-toxic and hepatoprotective in nature. Biochemical investigations (SGOT, SGPT) further corroborated these cytological observations. Conclusions: It can be concluded from this study that F. limonia methanol extract, some fractions and pure isolated compound herein exhibit hepatoprotective activity. However, cytotoxicity recorded in the penultimate fraction and investigation of structural details of pure compound warrants further study.

KW - Cytotoxicity

KW - Feronia limonia leaf

KW - HPLC

KW - HepG2

KW - Hepatoprotective

KW - Hepatoprotective activity

KW - Isolated compound

KW - Phytochemical analysis

KW - SGOT

KW - SGPT

KW - Spectroscopic assay

KW - TLC

UR - http://www.scopus.com/inward/record.url?scp=79959389954&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959389954&partnerID=8YFLogxK

U2 - 10.1016/S2221-1691(11)60097-X

DO - 10.1016/S2221-1691(11)60097-X

M3 - Article

C2 - 23569810

AN - SCOPUS:79959389954

VL - 1

SP - 443

EP - 447

JO - Asian Pacific Journal of Tropical Biomedicine

JF - Asian Pacific Journal of Tropical Biomedicine

SN - 2221-1691

IS - 6

ER -