Cytotoxicity of adhesive systems of different hydrophilicities on cultured odontoblast-like cells

Luciana Bianchi, Ana Paula Dias Ribeiro, Marcela Rocha De Oliveira Carrilho, David H. Pashley, Carlos Alberto De Souza Costa, Josimeri Hebling

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study evaluated the cytotoxicity of experimental adhesive systems (EASs) on odontoblast-like cells. Paper discs (n = 132) were impregnated with 10 μL of each EAS - R1, R2, R3, R4, and R5 (in an ascending order of hydrophilicity), followed by photoactivation. R1 and R2 are nonsolvated hydrophobic blends, R3 represents a simplified etch-and-rinse adhesive system, and R4 and R5 represent simplified self-etch adhesive systems. Discs were immersed in Dulbecco's modified Eagle's medium for 24 h to obtain eluates applied on MDPC-23 cell cultures. No material was applied on discs used as control (R0). Cell viability [3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay], total protein (TP) production, alkaline phosphatase (ALP) activity, type of cell death, and degree of monomer conversion Fourier transform infrared (%DC-FTIR) were evaluated. Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (α = 0.05). Considering R0 (control) as having 100% of cell viability, R1, R2, R3, R4, and R5 reduced the metabolic activity of cells by 36.4, 3.1, 0.2, 21.5, and 65.7%, respectively, but only R1 and R5 differed from R0. Comparing with R0, lower TP production was observed for R1, R4, and R5, while ALP activity decreased for R1 and R5. Necrotic cell death was predominant for all EASs, but only R1, R4, and R5 differed from R0. Only R5 presented a different apoptotic cell death ratio from R0. R1 presented the lowest %DC (ca. 37%), whereas R4 and R5 presented the highest (ca. 56%). In conclusion, R2 and R3 were not toxic to the MDPC-23 cells, suggesting that the degree of hydrophilicity or %DC of the EASs alone were not responsible for their cytopathic effects. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 101B: 1498-1507, 2013.

Original languageEnglish (US)
Pages (from-to)1498-1507
Number of pages10
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume101
Issue number8
DOIs
StatePublished - Jun 11 2013

Fingerprint

Odontoblasts
Hydrophilicity
Cytotoxicity
Hydrophobic and Hydrophilic Interactions
Adhesives
Cell death
Cell Death
Phosphatases
Cells
Alkaline Phosphatase
Cell Survival
Proteins
Eagles
Poisons
Fourier Analysis
Bromides
Cell culture
Assays
Fourier transforms
Cell Culture Techniques

Keywords

  • adhesive system
  • cytotoxicity
  • hydrophilicity
  • odontoblast-like MDPC-23 cells

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

Bianchi, L., Ribeiro, A. P. D., De Oliveira Carrilho, M. R., Pashley, D. H., De Souza Costa, C. A., & Hebling, J. (2013). Cytotoxicity of adhesive systems of different hydrophilicities on cultured odontoblast-like cells. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 101(8), 1498-1507. https://doi.org/10.1002/jbm.b.32971

Cytotoxicity of adhesive systems of different hydrophilicities on cultured odontoblast-like cells. / Bianchi, Luciana; Ribeiro, Ana Paula Dias; De Oliveira Carrilho, Marcela Rocha; Pashley, David H.; De Souza Costa, Carlos Alberto; Hebling, Josimeri.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 101, No. 8, 11.06.2013, p. 1498-1507.

Research output: Contribution to journalArticle

Bianchi, L, Ribeiro, APD, De Oliveira Carrilho, MR, Pashley, DH, De Souza Costa, CA & Hebling, J 2013, 'Cytotoxicity of adhesive systems of different hydrophilicities on cultured odontoblast-like cells', Journal of Biomedical Materials Research - Part B Applied Biomaterials, vol. 101, no. 8, pp. 1498-1507. https://doi.org/10.1002/jbm.b.32971
Bianchi, Luciana ; Ribeiro, Ana Paula Dias ; De Oliveira Carrilho, Marcela Rocha ; Pashley, David H. ; De Souza Costa, Carlos Alberto ; Hebling, Josimeri. / Cytotoxicity of adhesive systems of different hydrophilicities on cultured odontoblast-like cells. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2013 ; Vol. 101, No. 8. pp. 1498-1507.
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