Cytotoxicity of endodontic materials over 6-weeks ex vivo

Martha G Brackett, A. Marshall, P. E. Lockwood, J. B. Lewis, Regina L W Messer, S. Bouillaguet, J. C. Wataha

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Aim: To test the hypothesis that extending the time of a traditional ex vivo cytotoxicity test helps to identify trends in the behaviour of root core materials and sealers, which could ultimately aid in predicting their clinical safety and performance. Methodology: Endodontic sealers and core specimens were initially tested in direct contact with L929 fibroblasts for 72 h. Cell response was estimated by measuring cellular succinate dehydrogenase activity relative to Teflon controls. Cytotoxicity (% of more active cells) was reassessed after 1, 3, 4 and 6 weeks, with the specimens stored in a physiologically balanced salt-solution between tests. Results: Distinct trends in cytotoxicity among both core materials and sealers were observed over the 6-week test. Four of the six sealers and two of the three core materials showed cell viabilities of <30% of Teflon after 6 weeks (>70% cytotoxicity). Conclusions: The current results suggest that some endodontic materials have an elevated biological risk for extended intervals.

Original languageEnglish (US)
Pages (from-to)1072-1078
Number of pages7
JournalInternational Endodontic Journal
Volume41
Issue number12
DOIs
StatePublished - Dec 1 2008

Keywords

  • Cell-culture
  • MTT
  • Periapical inflammation
  • Polymers
  • Pulpal infection
  • Succinate dehydrogenase

ASJC Scopus subject areas

  • Dentistry(all)

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