DAF/Crry double deficiency in mice exacerbates nephrotoxic serum-induced proteinuria despite markedly reduced systemic complement activity

Takashi Miwa, Lin Zhou, Ruxandra Tudoran, John D. Lambris, Michael P. Madaio, Masaomi Nangaku, Hector Molina, Wen Chao Song

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Decay-accelerating factor (DAF) and complement receptor 1-related gene/protein y (Crry) are two membrane-anchored complement regulatory proteins in rodent. Although both proteins are broadly distributed and exert complement regulation at the same steps of the complement cascade, DAF knockout mice are viable whereas Crry knockout mice die in utero as a result of maternal complement attack. The latter outcome has prevented the dissection of overlapping functions of DAF and Crry in adult mouse tissues in vivo. By crossing female DAF-/-/Crry-/-/C3-/- mice with male DAF-/-/Crry+/-/C3+/- mice, we circumvented maternal complement attack during fetal development and generated viable DAF-/-/Crry-/-/C3+/- mice to address the consequence of DAF/Crry double deficiency. DAF-/-/Crry-/-/C3+/- mice were born at the expected frequency and survived to adulthood. However, they were found to have greatly reduced systemic complement activity due, at least in part, to spontaneous C3 activation and consumption. Plasma C3 proteins in DAF-/-/Crry-/-/C3+/- mice were 30% of that of wild-type mice, and serum complement activity, as assessed by zymosan and immune complex C3 opsonization assays, was 90% reduced in DAF-/-/Crry-/-/C3+/- mice. Remarkably, despite greatly reduced systemic complement activity, DAF-/-/Crry-/-/C3+/- mice developed more severe proteinuria after induction of nephrotoxic serum nephritis as compared with DAF-/-/Crry+/-/C3+/- and DAF-/-/Crry-/-/C3-/- littermate controls. The results highlight the critical and overlapping role of Crry and DAF in vivo in preventing complement activation and tissue injury.

Original languageEnglish (US)
Pages (from-to)139-146
Number of pages8
JournalMolecular Immunology
Volume44
Issue number1-3
DOIs
StatePublished - Jan 1 2007

Fingerprint

CD55 Antigens
Proteinuria
Serum
Knockout Mice
Mothers
Complement C1
Complement Receptors
Zymosan
Nephritis
Complement Activation
Fetal Development

Keywords

  • Anti-GBM
  • CD55
  • Complement
  • Crry
  • DAF
  • Glomerulonephritis
  • Inflammation

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology

Cite this

DAF/Crry double deficiency in mice exacerbates nephrotoxic serum-induced proteinuria despite markedly reduced systemic complement activity. / Miwa, Takashi; Zhou, Lin; Tudoran, Ruxandra; Lambris, John D.; Madaio, Michael P.; Nangaku, Masaomi; Molina, Hector; Song, Wen Chao.

In: Molecular Immunology, Vol. 44, No. 1-3, 01.01.2007, p. 139-146.

Research output: Contribution to journalArticle

Miwa, Takashi ; Zhou, Lin ; Tudoran, Ruxandra ; Lambris, John D. ; Madaio, Michael P. ; Nangaku, Masaomi ; Molina, Hector ; Song, Wen Chao. / DAF/Crry double deficiency in mice exacerbates nephrotoxic serum-induced proteinuria despite markedly reduced systemic complement activity. In: Molecular Immunology. 2007 ; Vol. 44, No. 1-3. pp. 139-146.
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AU - Zhou, Lin

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AU - Lambris, John D.

AU - Madaio, Michael P.

AU - Nangaku, Masaomi

AU - Molina, Hector

AU - Song, Wen Chao

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