DCLK1 Is Detectable in Plasma of Patients with Barrett’s Esophagus and Esophageal Adenocarcinoma

Joshua Whorton, Sripathi M. Sureban, Randal May, Dongfeng Qu, Stan A. Lightfoot, Mohammad Madhoun, Milton Johnson, William M. Tierney, John T. Maple, Kenneth J Vega, Courtney W. Houchen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Doublecortin-like kinase 1 (DCLK1), a putative tumor stem cell marker has been shown to be highly expressed in the stromal and epithelial compartments in colon and pancreatic cancer as well as Barrett’s esophagus (BE) and esophageal adenocarcinoma (EAC).

Aim: To prospectively investigate whether the immunohistochemical expression of DCLK1 was associated with detectable DCLK1 plasma expression in patients with existing BE and EAC.

Methods: Immunohistochemistry was performed on paraffin-embedded sections using DCLK1 antibody and scored based on staining intensity and tissue involvement. Purified human plasma samples were subjected to Western blot and ELISA analysis.

Results: Forty (40) patients were enrolled: 10 controls (normal endoscopy) and 30 with BE/EAC (13 nondysplastic BE [NDBE], 9 dysplastic BE [DBE] and 8 EAC). Mean epithelial DCLK1 staining was as follows: controls = 0.11, NDBE = 3.83, DBE = 6.0, EAC = 7.17. Mean stromal DCLK1 staining was as follows: NDBE = 5.83, DBE = 5.375, EAC = 10.83. DCLK1 was detected by plasma Western blot in 1 control and in all patients with BE/EAC p < 0.0005. Plasma DCLK1 was elevated by ELISA in EAC compared to other groups, p < 0.05.

Conclusions: Increased expression of DCLK1 was observed in the epithelium, stroma and plasma of patients with BE/EAC. Furthermore, the presence of detectable DCLK1 in plasma of BE/EAC patients may provide a less invasive, detection tool in those patients as well as represent a novel molecular marker distinguishing between normal esophageal mucosa and BE or EAC.

Original languageEnglish (US)
Pages (from-to)509-513
Number of pages5
JournalDigestive Diseases and Sciences
Volume60
Issue number2
DOIs
StatePublished - Jan 23 2015
Externally publishedYes

Fingerprint

Barrett Esophagus
Adenocarcinoma
Phosphotransferases
Staining and Labeling
Western Blotting
Enzyme-Linked Immunosorbent Assay
Neoplastic Stem Cells
Esophageal Neoplasms
Pancreatic Neoplasms
Paraffin
Colonic Neoplasms
Endoscopy
Epithelium
Immunohistochemistry

Keywords

  • Barrett’s esophagus
  • DCLK1
  • Esophageal adenocarcinoma
  • Serum biomarker
  • Tumor stem cell marker

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Whorton, J., Sureban, S. M., May, R., Qu, D., Lightfoot, S. A., Madhoun, M., ... Houchen, C. W. (2015). DCLK1 Is Detectable in Plasma of Patients with Barrett’s Esophagus and Esophageal Adenocarcinoma. Digestive Diseases and Sciences, 60(2), 509-513. https://doi.org/10.1007/s10620-014-3347-4

DCLK1 Is Detectable in Plasma of Patients with Barrett’s Esophagus and Esophageal Adenocarcinoma. / Whorton, Joshua; Sureban, Sripathi M.; May, Randal; Qu, Dongfeng; Lightfoot, Stan A.; Madhoun, Mohammad; Johnson, Milton; Tierney, William M.; Maple, John T.; Vega, Kenneth J; Houchen, Courtney W.

In: Digestive Diseases and Sciences, Vol. 60, No. 2, 23.01.2015, p. 509-513.

Research output: Contribution to journalArticle

Whorton, J, Sureban, SM, May, R, Qu, D, Lightfoot, SA, Madhoun, M, Johnson, M, Tierney, WM, Maple, JT, Vega, KJ & Houchen, CW 2015, 'DCLK1 Is Detectable in Plasma of Patients with Barrett’s Esophagus and Esophageal Adenocarcinoma', Digestive Diseases and Sciences, vol. 60, no. 2, pp. 509-513. https://doi.org/10.1007/s10620-014-3347-4
Whorton, Joshua ; Sureban, Sripathi M. ; May, Randal ; Qu, Dongfeng ; Lightfoot, Stan A. ; Madhoun, Mohammad ; Johnson, Milton ; Tierney, William M. ; Maple, John T. ; Vega, Kenneth J ; Houchen, Courtney W. / DCLK1 Is Detectable in Plasma of Patients with Barrett’s Esophagus and Esophageal Adenocarcinoma. In: Digestive Diseases and Sciences. 2015 ; Vol. 60, No. 2. pp. 509-513.
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AU - Whorton, Joshua

AU - Sureban, Sripathi M.

AU - May, Randal

AU - Qu, Dongfeng

AU - Lightfoot, Stan A.

AU - Madhoun, Mohammad

AU - Johnson, Milton

AU - Tierney, William M.

AU - Maple, John T.

AU - Vega, Kenneth J

AU - Houchen, Courtney W.

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N2 - Background: Doublecortin-like kinase 1 (DCLK1), a putative tumor stem cell marker has been shown to be highly expressed in the stromal and epithelial compartments in colon and pancreatic cancer as well as Barrett’s esophagus (BE) and esophageal adenocarcinoma (EAC).Aim: To prospectively investigate whether the immunohistochemical expression of DCLK1 was associated with detectable DCLK1 plasma expression in patients with existing BE and EAC.Methods: Immunohistochemistry was performed on paraffin-embedded sections using DCLK1 antibody and scored based on staining intensity and tissue involvement. Purified human plasma samples were subjected to Western blot and ELISA analysis.Results: Forty (40) patients were enrolled: 10 controls (normal endoscopy) and 30 with BE/EAC (13 nondysplastic BE [NDBE], 9 dysplastic BE [DBE] and 8 EAC). Mean epithelial DCLK1 staining was as follows: controls = 0.11, NDBE = 3.83, DBE = 6.0, EAC = 7.17. Mean stromal DCLK1 staining was as follows: NDBE = 5.83, DBE = 5.375, EAC = 10.83. DCLK1 was detected by plasma Western blot in 1 control and in all patients with BE/EAC p < 0.0005. Plasma DCLK1 was elevated by ELISA in EAC compared to other groups, p < 0.05.Conclusions: Increased expression of DCLK1 was observed in the epithelium, stroma and plasma of patients with BE/EAC. Furthermore, the presence of detectable DCLK1 in plasma of BE/EAC patients may provide a less invasive, detection tool in those patients as well as represent a novel molecular marker distinguishing between normal esophageal mucosa and BE or EAC.

AB - Background: Doublecortin-like kinase 1 (DCLK1), a putative tumor stem cell marker has been shown to be highly expressed in the stromal and epithelial compartments in colon and pancreatic cancer as well as Barrett’s esophagus (BE) and esophageal adenocarcinoma (EAC).Aim: To prospectively investigate whether the immunohistochemical expression of DCLK1 was associated with detectable DCLK1 plasma expression in patients with existing BE and EAC.Methods: Immunohistochemistry was performed on paraffin-embedded sections using DCLK1 antibody and scored based on staining intensity and tissue involvement. Purified human plasma samples were subjected to Western blot and ELISA analysis.Results: Forty (40) patients were enrolled: 10 controls (normal endoscopy) and 30 with BE/EAC (13 nondysplastic BE [NDBE], 9 dysplastic BE [DBE] and 8 EAC). Mean epithelial DCLK1 staining was as follows: controls = 0.11, NDBE = 3.83, DBE = 6.0, EAC = 7.17. Mean stromal DCLK1 staining was as follows: NDBE = 5.83, DBE = 5.375, EAC = 10.83. DCLK1 was detected by plasma Western blot in 1 control and in all patients with BE/EAC p < 0.0005. Plasma DCLK1 was elevated by ELISA in EAC compared to other groups, p < 0.05.Conclusions: Increased expression of DCLK1 was observed in the epithelium, stroma and plasma of patients with BE/EAC. Furthermore, the presence of detectable DCLK1 in plasma of BE/EAC patients may provide a less invasive, detection tool in those patients as well as represent a novel molecular marker distinguishing between normal esophageal mucosa and BE or EAC.

KW - Barrett’s esophagus

KW - DCLK1

KW - Esophageal adenocarcinoma

KW - Serum biomarker

KW - Tumor stem cell marker

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