35 yaş üzeri in-vitro fertilizasyon over follikül sıvı proteomların çözümlenmesi

Translated title of the contribution: Decoding the proteome of In-Vitro fertilization ovarian follicular fluid for women over 35 years

Sabry Mohamed, Ibrahim M.A. Hassanin, Abdou Saeed Ait-Allah, Sabri Mahmoud Mohamed, Yaser A. Helmy, Ayman Al-Hendy

Research output: Contribution to journalArticle

1 Scopus citations


Aim: The study of follicular fluid using proteomic techniques could provide a useful tool for understanding follicular fluid components and their effect on pregnancy outcome. The aim of the study is to identify and catalog follicular fluid proteins in women 35 years of age or older. Material and Method: Follicular fluid was collected from 21 couples, of which 11 couples achieved successful pregnancy and 10 couples failed to get pregnant. Samples were analyzed by multidimensional chromatography coupled with in-line nanospray ionization mass spectrometry on an LTQ XL ion trap mass spectrometer. We used the Biomarker Analysis Program from PDQuest software to identify protein constituents in pregnant and non-pregnant groups. Results: In total, 1024 protein specimens were identified. The proteins identified were consistent throughout the experiment and within each of the analyzed specimens. Discussion: A compiled listing of follicular fluid proteins could be a potential starting point for the identification and evaluation of important proteins involved in the development of oocytes; the results of our study may fill a noticeable knowledge-gap in the understanding of follicular fluid proteome.

Original languageTurkish
Pages (from-to)60-64
Number of pages5
JournalJournal of Clinical and Analytical Medicine
Issue number1
StatePublished - Jan 2016



  • Follicular fluid
  • In-vitro fertilization
  • Proteome

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Mohamed, S., Hassanin, I. M. A., Ait-Allah, A. S., Mohamed, S. M., Helmy, Y. A., & Al-Hendy, A. (2016). 35 yaş üzeri in-vitro fertilizasyon over follikül sıvı proteomların çözümlenmesi. Journal of Clinical and Analytical Medicine, 7(1), 60-64. https://doi.org/10.4328/JCAM.3417