Decreased protein nitration in macrophages that overexpress indoleamine 2, 3-dioxygenase

Derin B. Keskin, Brendan Marshall, David Munn, Andrew L. Mellor, Debra A. Gearhart

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The activity of indoleamine 2, 3-dioxygenase (IDO; E.C. 1.13.11.42) catalyzes the oxidative cleavage of tryptophan to form kynurenine. IDO activity consumes superoxide anions; therefore, we postulated that over-expression of IDO might mitigate superoxide-anion dependent, oxidative modification of cellular proteins in vitro. We prepared and characterized RAW 264.7 macrophages that were stably transfected with either an IDO expression vector or the control (empty) vector. We detected IDO mRNA, protein, and enzyme activity in the IDO-transfected macrophages, but not in the macrophages transfected with the empty vector. To generate superoxide anions in situ, we treated the IDO- and control-transfected cultures with xanthine or hypoxanthine, and then used ELISA methods to quantitate the relative levels of oxidatively modified proteins in total cell lysates. The levels of protein carbonyls were similar in IDO-transfected and vector-transfected macrophages; however, protein nitration was significantly less in IDO-transfected cells compared to control transfectants. In addition, steady-state levels of superoxide anions were significantly lower in the IDO-transfected cultures compared with control transfectants. Our results are consistent with the concept that, besides degrading tryptophan, IDO activity may protect cells from oxidative damage.

Original languageEnglish (US)
Pages (from-to)82-102
Number of pages21
JournalCellular and Molecular Biology Letters
Volume12
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Indoleamine-Pyrrole 2,3,-Dioxygenase
Nitration
Macrophages
Superoxides
Proteins
Tryptophan
Kynurenine
Hypoxanthine
Xanthine
Enzyme activity
Enzyme-Linked Immunosorbent Assay
Messenger RNA
Enzymes

Keywords

  • Indoleamine 2, 3-dioxygenase
  • Oxidative stress
  • Protein oxidation
  • RAW 264.7 macrophages
  • Superoxide anion

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Decreased protein nitration in macrophages that overexpress indoleamine 2, 3-dioxygenase. / Keskin, Derin B.; Marshall, Brendan; Munn, David; Mellor, Andrew L.; Gearhart, Debra A.

In: Cellular and Molecular Biology Letters, Vol. 12, No. 1, 01.01.2007, p. 82-102.

Research output: Contribution to journalArticle

Keskin, Derin B. ; Marshall, Brendan ; Munn, David ; Mellor, Andrew L. ; Gearhart, Debra A. / Decreased protein nitration in macrophages that overexpress indoleamine 2, 3-dioxygenase. In: Cellular and Molecular Biology Letters. 2007 ; Vol. 12, No. 1. pp. 82-102.
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