Defective antibody-mediated opsonisation of S. pneumoniae in high risk patients detected by flow cytometry

S. K. Obaro, D. C. Henderson, Michele Anne Monteil

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Invasive pneumococcal infection continues to be a significant cause of morbidity and mortality especially in patients with antibody deficiencies and disorders affecting the reticulo-endothelial system. Current recommendations for prophylaxis in these patients include immunization with 23-valent pneumococcal polysaccharide capsular vaccines. Post-immunization responses are commonly assessed by measuring serum antibody levels. However, there is no defined protective antibody range and this approach fails to determine the functional capacity of the antibodies. A simple, reproducible flow cytometric method of assessing the antibody-mediated opsonic activity against S. pneumoniae is described. This assay detected defective opsonic function in post-immunized at-risk patients who developed invasive pneumococcal infection.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalImmunology Letters
Volume49
Issue number1-2
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Pneumonia
Flow Cytometry
Antibodies
Pneumococcal Infections
Immunization
Morbidity
Mortality
Serum

Keywords

  • Flow cytometry
  • Functional antibody deficiency
  • Opsonisation
  • Phagocytosis
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Defective antibody-mediated opsonisation of S. pneumoniae in high risk patients detected by flow cytometry. / Obaro, S. K.; Henderson, D. C.; Monteil, Michele Anne.

In: Immunology Letters, Vol. 49, No. 1-2, 01.01.1996, p. 83-89.

Research output: Contribution to journalArticle

Obaro, S. K. ; Henderson, D. C. ; Monteil, Michele Anne. / Defective antibody-mediated opsonisation of S. pneumoniae in high risk patients detected by flow cytometry. In: Immunology Letters. 1996 ; Vol. 49, No. 1-2. pp. 83-89.
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