Defective antibody-mediated opsonisation of S. pneumoniae in high risk patients detected by flow cytometry

S. K. Obaro, D. C. Henderson, M. A. Monteil

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Invasive pneumococcal infection continues to be a significant cause of morbidity and mortality especially in patients with antibody deficiencies and disorders affecting the reticulo-endothelial system. Current recommendations for prophylaxis in these patients include immunization with 23-valent pneumococcal polysaccharide capsular vaccines. Post-immunization responses are commonly assessed by measuring serum antibody levels. However, there is no defined protective antibody range and this approach fails to determine the functional capacity of the antibodies. A simple, reproducible flow cytometric method of assessing the antibody-mediated opsonic activity against S. pneumoniae is described. This assay detected defective opsonic function in post-immunized at-risk patients who developed invasive pneumococcal infection.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalImmunology Letters
Volume49
Issue number1-2
DOIs
Publication statusPublished - Jan 1996

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Keywords

  • Flow cytometry
  • Functional antibody deficiency
  • Opsonisation
  • Phagocytosis
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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