Defining the biomechanical properties of the rabbit trachea

Matthew C. Jones, Frederick Rueggeberg, Hunter Allen Faircloth, Aaron J. Cunningham, Carrie M. Bush, John Drew Prosser, Jennifer L Waller, Gregory N Postma, Paul Maurice Weinberger

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objectives/Hypothesis: Surgical advancements rely heavily on validated animal models. The New Zealand White (NZW) rabbit is a widely used model for airway research, including regenerative medicine applications. Currently, the biomechanical properties of the normal rabbit trachea are not known. Our objective was to define these properties to assist in the standardization and understanding of future airway research using this model. Study Design: Laboratory-based study. Methods: Fresh tracheas from four adult NZW rabbits were dissected into 20 segments. To examine the biomechanical properties, segments were subjected to uniaxial tension (n = 9) and compression (n = 11) testing. Yield and maximum load (tension) and force at 50% displacement (compression) were recorded, and differences between segments were examined using analysis of covariance. Results: Normative data for native rabbit trachea show mean maximum load = 6.44 newtons (N), yield load = 5.93 N, and compressive strength = 2.10 N. In addition to establishing the baseline measurements, statistically significant differences in tensile measures based on location along the trachea and diameter were identified. Proximal segments had significantly higher maximum load (P = .0029) and yield load (P = .0062) than distal segments. Association between diameter and both maximum load (P = .0139) and yield load (P = .0082) was observed. Conclusions: The adult NZW rabbit trachea is intrinsically less able to withstand tensile and compressive forces, compared to other airway models such as sheep or cadaveric human. Establishment of normative values will enable future research into changes in tracheal biomechanical properties during regenerative medicine manipulation and processing.

Original languageEnglish (US)
Pages (from-to)2352-2358
Number of pages7
JournalLaryngoscope
Volume124
Issue number10
DOIs
StatePublished - Oct 1 2014

Fingerprint

Trachea
Rabbits
Regenerative Medicine
Compressive Strength
Research
Sheep
Animal Models

Keywords

  • Allograft
  • Biomechanical properties
  • Biomechanics
  • New Zealand White rabbit
  • Structural properties
  • Tensile strength
  • Tissue engineering
  • Trachea
  • Tracheal transplant
  • Tracheal transplantation

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Medicine(all)

Cite this

Defining the biomechanical properties of the rabbit trachea. / Jones, Matthew C.; Rueggeberg, Frederick; Faircloth, Hunter Allen; Cunningham, Aaron J.; Bush, Carrie M.; Prosser, John Drew; Waller, Jennifer L; Postma, Gregory N; Weinberger, Paul Maurice.

In: Laryngoscope, Vol. 124, No. 10, 01.10.2014, p. 2352-2358.

Research output: Contribution to journalArticle

Jones, Matthew C. ; Rueggeberg, Frederick ; Faircloth, Hunter Allen ; Cunningham, Aaron J. ; Bush, Carrie M. ; Prosser, John Drew ; Waller, Jennifer L ; Postma, Gregory N ; Weinberger, Paul Maurice. / Defining the biomechanical properties of the rabbit trachea. In: Laryngoscope. 2014 ; Vol. 124, No. 10. pp. 2352-2358.
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abstract = "Objectives/Hypothesis: Surgical advancements rely heavily on validated animal models. The New Zealand White (NZW) rabbit is a widely used model for airway research, including regenerative medicine applications. Currently, the biomechanical properties of the normal rabbit trachea are not known. Our objective was to define these properties to assist in the standardization and understanding of future airway research using this model. Study Design: Laboratory-based study. Methods: Fresh tracheas from four adult NZW rabbits were dissected into 20 segments. To examine the biomechanical properties, segments were subjected to uniaxial tension (n = 9) and compression (n = 11) testing. Yield and maximum load (tension) and force at 50{\%} displacement (compression) were recorded, and differences between segments were examined using analysis of covariance. Results: Normative data for native rabbit trachea show mean maximum load = 6.44 newtons (N), yield load = 5.93 N, and compressive strength = 2.10 N. In addition to establishing the baseline measurements, statistically significant differences in tensile measures based on location along the trachea and diameter were identified. Proximal segments had significantly higher maximum load (P = .0029) and yield load (P = .0062) than distal segments. Association between diameter and both maximum load (P = .0139) and yield load (P = .0082) was observed. Conclusions: The adult NZW rabbit trachea is intrinsically less able to withstand tensile and compressive forces, compared to other airway models such as sheep or cadaveric human. Establishment of normative values will enable future research into changes in tracheal biomechanical properties during regenerative medicine manipulation and processing.",
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AU - Postma, Gregory N

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KW - Tracheal transplant

KW - Tracheal transplantation

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