Deletion of microRNA miR-223 increases Langerhans cell cross-presentation

Qing Sheng Mi, Ying Ping Xu, He Wang, Rui Qun Qi, Zheng Dong, Li Zhou

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Langerhans cells (LCs) are skin-residential dendritic cells that regulate skin immunity. MicroRNAs (miRNAs) are key regulators in the control of biological functions in a variety of cell types. Deletion of all miRNAs interrupts the homeostasis and function of epidermal LCs. However, the roles of individual miRNAs in regulating LC development and function are still completely unknown. MiRNA miR-223 is especially expressed in the myeloid compartment. Here, we reported that miR-223 is highly expressed in freshly isolated epidermal LCs, and tested whether miR-223 regulates LC development and function using miR-223 knockout (KO) mice. We found that the number, maturation, migration and phagocytic capacity of LCs were comparable between miR-223KO and wild-type mice. However, lack of miR-223 significantly increases LCs-mediated antigen-specific CD8 + T cell proliferation in vivo and in vitro, while LCs from KO and WT mice showed comparable stimulation for antigen-specific CD4 + T cells. Our data suggest that miR-223 negatively regulates LC cross-presentation, but may not be required for normal LC homeostasis and development.

Original languageEnglish (US)
Pages (from-to)395-400
Number of pages6
JournalInternational Journal of Biochemistry and Cell Biology
Volume45
Issue number2
DOIs
StatePublished - Feb 1 2013

Fingerprint

Cross-Priming
Langerhans Cells
MicroRNAs
T-cells
Skin
Antigens
Cell proliferation
Knockout Mice
Homeostasis
CD8 Antigens
T-Lymphocytes
CD4 Antigens
Immunity

Keywords

  • Cross-presentation
  • Langerhans cells
  • MicroRNA
  • miR-223

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Deletion of microRNA miR-223 increases Langerhans cell cross-presentation. / Mi, Qing Sheng; Xu, Ying Ping; Wang, He; Qi, Rui Qun; Dong, Zheng; Zhou, Li.

In: International Journal of Biochemistry and Cell Biology, Vol. 45, No. 2, 01.02.2013, p. 395-400.

Research output: Contribution to journalArticle

Mi, Qing Sheng ; Xu, Ying Ping ; Wang, He ; Qi, Rui Qun ; Dong, Zheng ; Zhou, Li. / Deletion of microRNA miR-223 increases Langerhans cell cross-presentation. In: International Journal of Biochemistry and Cell Biology. 2013 ; Vol. 45, No. 2. pp. 395-400.
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