Deletion of protein kinase D1 in osteoprogenitor cells results in decreased osteogenesis in vitro and reduced bone mineral density in vivo

Wendy B. Bollag, Vivek Choudhary, Qing Zhong, Ke Hong Ding, Jianrui Xu, Ranya Elsayed, Kanglun Yu, Yun Su, Lakiea J. Bailey, Xing Ming Shi, Mohammed Elsalanty, Maribeth H. Johnson, Meghan E. McGee-Lawrence, Carlos M. Isales

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Abstract

Protein kinase D1 (PRKD1) is thought to play a role in a number of cellular functions, including proliferation and differentiation. We hypothesized that PRKD1 in bone marrow-derived mesenchymal stem cells (BMMSC) could modulate osteogenesis. In BMMSCs from floxed PRKD1 mice, PRKD1 ablation with adenovirus-mediated Cre-recombinase expression inhibited BMMSC differentiation in vitro. In 3- and 6-month-old conditional knockout mice (cKO), in which PRKD1 was ablated in osteoprogenitor cells by osterix promoter-driven Cre-recombinase, bone mineral density (BMD) was significantly reduced compared with floxed control littermates. Microcomputed tomography analysis also demonstrated a decrease in trabecular thickness and bone volume fraction in cKO mice at these ages. Dynamic bone histomorphometry suggested a mineralization defect in the cKO mice. However, by 9 months of age, the bone appeared to compensate for the lack of PRKD1, and BMD was not different. Taken together, these results suggest a potentially important role for PRKD1 in bone formation.

Original languageEnglish (US)
Pages (from-to)22-31
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume461
DOIs
StatePublished - Feb 5 2018

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Keywords

  • Bone
  • Mesenchymal stem cells
  • Mineralization
  • Osteoprogenitor
  • Osterix
  • Protein kinase D1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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