Delivering defined amounts of purified protein with high precision into living cells

Research output: Contribution to journalArticlepeer-review

Abstract

Here, we detail a protocol using electroporation to precisely deliver defined amounts of purified protein into CAD cells. This method allows one million cells to be electroporated with protein simultaneously, with high delivery efficiency and low cell death. Further, by circumventing the normal biosynthetic pathway, proteins can be studied without the complication of post-translational modifications and before a transcriptional response can be initiated. This protocol will be useful for any researcher who is interested in protein concentration-dependent cellular phenotypes. For complete details on the use and execution of this protocol, please refer to Skruber et al. (2020).

Original languageEnglish (US)
Article number100272
JournalSTAR Protocols
Volume2
Issue number1
DOIs
StatePublished - Mar 19 2021
Externally publishedYes

Keywords

  • Cell biology
  • Cell culture
  • Cell-based assays
  • Microscopy
  • Molecular/chemical probes
  • Protein expression and purification

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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