Denaturation Temperatures of Dentin Matrices. I. Effect of Demineralization and Dehydration

Steven R. Armstrong, Julie L P Jessop, Erik Winn, Franklin Chi Meng Tay, David Henry Pashley

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The denaturation temperature (Td) of dentin collagen in mineralized versus demineralized teeth was examined as a function of dentin age and the extent of dehydration. Using differential scanning calorimetry, Td of mineralized dentin was shown to be between 160°C to 186°C, depending on whether it was from young or old dentin that was hydrated or dehydrated, respectively. Demineralized dentin exhibited a Td of 65.6°C that increased with dehydration to 176°C. The presence of apatite crystallites or interpeptide bonding increased the Td of demineralized matrices. Interpeptide hydrogen bonding seems to stabilize collagen to thermal challenge. Water breaks interpeptide hydrogen bonds making collagen more susceptible to thermal denaturation. Rises in intracanal temperature are unlikely to cause extensive denaturation of mineralized root dentin walls. However, hydrated or partially dehydrated root canal walls that have been partially demineralized with chelating agents or mild acids may be susceptible to thermal denaturation.

Original languageEnglish (US)
Pages (from-to)638-641
Number of pages4
JournalJournal of Endodontics
Volume32
Issue number7
DOIs
StatePublished - Jul 1 2006

Fingerprint

Dentin
Dehydration
Temperature
Collagen
Hot Temperature
Apatites
Dental Pulp Cavity
Differential Scanning Calorimetry
Hydrogen Bonding
Chelating Agents
Hydrogen
Tooth
Acids
Water

Keywords

  • Collagen
  • dentin
  • differential scanning calorimetry
  • glass transition

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Denaturation Temperatures of Dentin Matrices. I. Effect of Demineralization and Dehydration. / Armstrong, Steven R.; Jessop, Julie L P; Winn, Erik; Tay, Franklin Chi Meng; Pashley, David Henry.

In: Journal of Endodontics, Vol. 32, No. 7, 01.07.2006, p. 638-641.

Research output: Contribution to journalArticle

Armstrong, Steven R. ; Jessop, Julie L P ; Winn, Erik ; Tay, Franklin Chi Meng ; Pashley, David Henry. / Denaturation Temperatures of Dentin Matrices. I. Effect of Demineralization and Dehydration. In: Journal of Endodontics. 2006 ; Vol. 32, No. 7. pp. 638-641.
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