Dendrimer-triglycine-EGF nanoparticles for tumor imaging and targeted nucleic acid and drug delivery

Quan Yuan, Eunmee Lee, William Andrew Yeudall, Hu Yang

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We designed an epidermal growth factor (EGF)-containing polyamidoamine (PAMAM) Generation 4 dendrimer vector labeled with quantum dots for targeted imaging and nucleic acid delivery. 1 H NMR, SDS-PAGE, and Western blotting were applied to characterize the synthesized G4.0-GGG-EGF nanoparticles. Targeting efficiency, cell viability, proliferation, and intracellular signal transduction were evaluated using HN12, NIH3T3, and NIH3T3/EGFR cells. We found that EGF-conjugated dendrimers did not stimulate growth of EGFR-expressing cells at the selected concentration. Consistent with this, minimal stimulation of post-receptor signaling pathways was observed. These nanoparticles can localize within cells that express the EGFR in a receptor-dependent manner, whereas uptake into cells lacking the receptor was low. A well characterized vimentin shRNA (shVIM) and yellow fluorescent protein (YFP) siRNA were used to test the delivery and transfection efficiency of the constructed targeted vector. Significant knockdown of expression was observed, indicating that this vector is useful for introduction of nucleic acids or drugs into cells by a receptor-targeted mechanism.

Original languageEnglish (US)
Pages (from-to)698-704
Number of pages7
JournalOral Oncology
Volume46
Issue number9
DOIs
StatePublished - Sep 1 2010
Externally publishedYes

Fingerprint

glycyl-glycyl-glycine
Dendrimers
Epidermal Growth Factor
Nanoparticles
Nucleic Acids
Pharmaceutical Preparations
Neoplasms
Small Interfering RNA
Quantum Dots
Vimentin
Transfection
Polyacrylamide Gel Electrophoresis
Signal Transduction
Cell Survival
Western Blotting
Cell Proliferation
Growth

Keywords

  • Dendrimer
  • EGF
  • Gene delivery
  • Gene knockdown
  • HNSCC
  • Imaging
  • Nanoparticles
  • Quantum dots
  • RNAi
  • SiRNA
  • Vimentin
  • YFP

ASJC Scopus subject areas

  • Oral Surgery
  • Oncology
  • Cancer Research

Cite this

Dendrimer-triglycine-EGF nanoparticles for tumor imaging and targeted nucleic acid and drug delivery. / Yuan, Quan; Lee, Eunmee; Yeudall, William Andrew; Yang, Hu.

In: Oral Oncology, Vol. 46, No. 9, 01.09.2010, p. 698-704.

Research output: Contribution to journalArticle

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