Dendritic cells (DC) facilitate detachment of squamous carcinoma cells (SCC), while SCC promote an immature CD16+ DC phenotype and control DC migration

Lalitha V. Ramanathapuram, Dustin Hopkin, Zoya Bronislavovna Kurago

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In the inflammatory mucosal microenvironment of head and neck SCC (HNSCC), DC express CD16 and are usually in direct contact with tumor cells. Mucosal and inflammation-associated DC develop from monocytes, and monocyte-derived DC are used in HNSCC immunotherapy. However, beyond apoptotic tumor cell uptake and presentation of tumor antigens by DC, HNSCC cell interactions with DC are poorly understood. Using co-cultures of monocyte-derived DC and two established HNSCC cell lines that represent well- and poorly-differentiated SCC, respectively, we found that carcinoma cells induced significant increases in CD16 expression on DC while promoting a CD1a+CD86dim immature phenotype, similar to that observed in HNSCC specimens. Moreover, HNSCC cells affected steady-state and CCL21-induced migration of DC, and these effects were donor-dependent. The CCL21-induced migration directly correlated with HNSCC-mediated effects on CCR7 and CD38 expression on DC-SIGN-high DC. The dominant pattern seen in six out of nine donors was the increase in steady-state and CCL21-induced DC migration in co-cultures with HNSCC, while the reverse pattern, i.e., decreased DC migration in co-cultures with SCC, was identified in two donors. A split in migratory DC behavior, i.e. increase with one HNSCC cell line and a decrease with the second cell line, was observed in one donor. Remarkably, the numbers of live detached HNSCC cells were orders of magnitude higher in DC-HNSCC co-cultures than in parallel HNSCC cell cultures without DC. This study provides novel insights into the effects of DC-HNSCC interactions relevant to the tumor microenvironment.

Original languageEnglish (US)
Pages (from-to)41-55
Number of pages15
JournalCancer Microenvironment
Volume6
Issue number1
DOIs
StatePublished - Apr 1 2013
Externally publishedYes

Fingerprint

Dendritic Cells
Cell Movement
Squamous Cell Carcinoma
Phenotype
Neck
Head
Coculture Techniques
Monocytes
Cell Line
Tumor Microenvironment
Neoplasm Antigens
Cell Communication
Immunotherapy
Neoplasms
Cell Culture Techniques

Keywords

  • CD16
  • Dendritic cells
  • Head and neck squamous cell carcinoma
  • Tumor microenvironment

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Dendritic cells (DC) facilitate detachment of squamous carcinoma cells (SCC), while SCC promote an immature CD16+ DC phenotype and control DC migration. / Ramanathapuram, Lalitha V.; Hopkin, Dustin; Kurago, Zoya Bronislavovna.

In: Cancer Microenvironment, Vol. 6, No. 1, 01.04.2013, p. 41-55.

Research output: Contribution to journalArticle

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