Depletion of ixba causes nitric oxide (no) production in rat aortic smooth muscle cells

C. Go, Y. Fei, G. Liou, J. D. Calravas

Research output: Contribution to journalArticle

Abstract

NO production in RASM stimulated with interleukin-l (IL-1β) involves the activation of nuclear factor-K (NF-KB} (Mülsch et al. Faseh J 9: A556.1995). Previously we reported the induction of NO synlhase ( iNOS ) and NO production by the protein synthesis inhibitor cycloheximide (CH), independently of other known inducers. Moreover. CH caused a laic-onset superinduction of lipopolysaccharide (LPS)-stimulated iNOS activity. These findings suggest the involvement of a CH-sensitive inhibitor in the activation of NOS. We hypothesized that protein synthesis inhibition by CH reduces levels of 1KB, a labile inhibitor of NF-KB. leading to NF KB activation and subsequent iNOS gene expression. Western blot analysis of RASM treated with lOuM CH and I0ng/ml LPS for 24hrs revealed elevated iNOS protein levels. In addition, CH caused a lime dependent decline in 1KB protein levels and a concommitant rise of a slower migrating isolbmi. Both the increase in iNOS activity and decline in 1KB protein levels by CH were significantly reduced by various protease inhibitors. Electrophoretic mobility shift assays confirmed that CH alone activated NFKB and augmented LPS-activaled NpKB. These results provide evidence that reduction in licBa basal levels leads to increased NF-KB-mediated iNOS gene expression and enzyme activity. (Supponed by HI, 52458L.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - Dec 1 1996

Fingerprint

cycloheximide
Cycloheximide
smooth muscle
myocytes
Smooth Muscle Myocytes
nitric oxide
Muscle
Rats
Nitric Oxide
Cells
rats
lipopolysaccharides
Lipopolysaccharides
Chemical activation
Gene expression
Proteins
Gene Expression
protein synthesis inhibitors
gene expression
Electrophoretic mobility

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Depletion of ixba causes nitric oxide (no) production in rat aortic smooth muscle cells. / Go, C.; Fei, Y.; Liou, G.; Calravas, J. D.

In: FASEB Journal, Vol. 10, No. 3, 01.12.1996.

Research output: Contribution to journalArticle

Go, C. ; Fei, Y. ; Liou, G. ; Calravas, J. D. / Depletion of ixba causes nitric oxide (no) production in rat aortic smooth muscle cells. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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