TY - JOUR
T1 - Depolarization and cardiolipin depletion in aged rat brain mitochondria
T2 - Relationship with oxidative stress and electron transport chain activity
AU - Sen, Tanusree
AU - Sen, Nilkantha
AU - Jana, Sirsendu
AU - Khan, Firoj Hossain
AU - Chatterjee, Uttara
AU - Chakrabarti, Sasanka
N1 - Funding Information:
This work is supported by a research grant from DST (SR/SO/HS-05/2004), Government of India, New Delhi. We acknowledge the kind help and suggestions of Dr. K.P. Mohanakumar, Indian Institute of Chemical Biology, Calcutta.
PY - 2007/4
Y1 - 2007/4
N2 - A noticeable loss of cardiolipin, a significant accumulation of fluorescent products of lipid peroxidation and an increased ability to produce reactive oxygen species in vitro are characteristics of aged rat brain mitochondria, as has been demonstrated in this study. In contrast mitochondrial electron transport chain activity is not significantly compromised except a marginal decline in complex IV activity in aged rat brain. On the other hand, a striking loss of mitochondrial membrane potential occurs in brain mitochondria during aging, which may be attributed to peroxidative membrane damage in this condition. Such mitochondrial dysfunctions as reported here may lead to uncoupling of oxidative phosphorylation, ATP depletion and activation of apoptotic cascade in aged rat brain.
AB - A noticeable loss of cardiolipin, a significant accumulation of fluorescent products of lipid peroxidation and an increased ability to produce reactive oxygen species in vitro are characteristics of aged rat brain mitochondria, as has been demonstrated in this study. In contrast mitochondrial electron transport chain activity is not significantly compromised except a marginal decline in complex IV activity in aged rat brain. On the other hand, a striking loss of mitochondrial membrane potential occurs in brain mitochondria during aging, which may be attributed to peroxidative membrane damage in this condition. Such mitochondrial dysfunctions as reported here may lead to uncoupling of oxidative phosphorylation, ATP depletion and activation of apoptotic cascade in aged rat brain.
KW - Brain aging
KW - Cardiolipin
KW - Electron transport chain
KW - Lipid peroxidation
KW - Mitochondria
KW - Mitochondrial membrane potential
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U2 - 10.1016/j.neuint.2007.01.007
DO - 10.1016/j.neuint.2007.01.007
M3 - Article
C2 - 17331620
AN - SCOPUS:33947508734
SN - 0197-0186
VL - 50
SP - 719
EP - 725
JO - Neurochemistry International
JF - Neurochemistry International
IS - 5
ER -