Deregulation of arginase induces bone complications in high-fat/high-sucrose diet diabetic mouse model

Anil Bhatta, Rajnikumar Sangani, Ravindra Bharat Kolhe, Haroldo Alfredo Flores Toque, Michael Cain, Abby Wong, Nicole Howie, Rahul Shinde, Mohammed Elsayed Elsalanty, Lin Yao, Norman Chutkan, Monty Hunter, Ruth B Caldwell, Carlos M Isales, Robert William Caldwell, Sadanand T Fulzele

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A balanced diet is crucial for healthy development and prevention of musculoskeletal related diseases. Diets high in fat content are known to cause obesity, diabetes and a number of other disease states. Our group and others have previously reported that activity of the urea cycle enzyme arginase is involved in diabetes-induced dysregulation of vascular function due to decreases in nitric oxide formation. We hypothesized that diabetes may also elevate arginase activity in bone and bone marrow, which could lead to bone-related complications. To test this we determined the effects of diabetes on expression and activity of arginase, in bone and bone marrow stromal cells (BMSCs). We demonstrated that arginase 1 is abundantly present in the bone and BMSCs. We also demonstrated that arginase activity and expression in bone and bone marrow is up-regulated in models of diabetes induced by HFHS diet and streptozotocin (STZ). HFHS diet down-regulated expression of healthy bone metabolism markers (BMP2, COL-1, ALP, and RUNX2) and reduced bone mineral density, bone volume and trabecular thickness. However, treatment with an arginase inhibitor (ABH) prevented these bone-related complications of diabetes. In-vitro study of BMSCs showed that high glucose treatment increased arginase activity and decreased nitric oxide production. These effects were reversed by treatment with an arginase inhibitor (ABH). Our study provides evidence that deregulation of l-arginine metabolism plays a vital role in HFHS diet-induced diabetic complications and that these complications can be prevented by treatment with arginase inhibitors. The modulation of l-arginine metabolism in disease could offer a novel therapeutic approach for osteoporosis and other musculoskeletal related diseases.

Original languageEnglish (US)
Pages (from-to)211-220
Number of pages10
JournalMolecular and Cellular Endocrinology
Volume422
DOIs
StatePublished - Feb 15 2016

Fingerprint

Arginase
Deregulation
High Fat Diet
Nutrition
Sucrose
Bone
Fats
Bone and Bones
Medical problems
Mesenchymal Stromal Cells
Musculoskeletal Diseases
Diet
Diabetes Complications
Metabolism
Arginine
Nitric Oxide
Bone Marrow
Activity Cycles
Streptozocin
Bone Density

Keywords

  • Arginase
  • Bone complications
  • Diabetic mouse model
  • High-fat/high-sucrose diet

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Deregulation of arginase induces bone complications in high-fat/high-sucrose diet diabetic mouse model. / Bhatta, Anil; Sangani, Rajnikumar; Kolhe, Ravindra Bharat; Flores Toque, Haroldo Alfredo; Cain, Michael; Wong, Abby; Howie, Nicole; Shinde, Rahul; Elsalanty, Mohammed Elsayed; Yao, Lin; Chutkan, Norman; Hunter, Monty; Caldwell, Ruth B; Isales, Carlos M; Caldwell, Robert William; Fulzele, Sadanand T.

In: Molecular and Cellular Endocrinology, Vol. 422, 15.02.2016, p. 211-220.

Research output: Contribution to journalArticle

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AU - Sangani, Rajnikumar

AU - Kolhe, Ravindra Bharat

AU - Flores Toque, Haroldo Alfredo

AU - Cain, Michael

AU - Wong, Abby

AU - Howie, Nicole

AU - Shinde, Rahul

AU - Elsalanty, Mohammed Elsayed

AU - Yao, Lin

AU - Chutkan, Norman

AU - Hunter, Monty

AU - Caldwell, Ruth B

AU - Isales, Carlos M

AU - Caldwell, Robert William

AU - Fulzele, Sadanand T

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