Detection of cell surface dopamine receptors

Jiping Xiao, Clare M Bergson

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Dopamine receptors are a class of metabotropic G protein-coupled receptors. Plasma membrane expression is a key determinant of receptor signaling, and one that is regulated both by extra and intracellular cues. Abnormal dopamine receptor signaling is implicated in several neuropsychiatric disorders, including schizophrenia and attention deficit hyperactivity disorder, as well as drug abuse. Here, we describe in detail the application of two complementary applications of protein biotinylation and enzyme-linked immunoabsorbent assay (ELISA) for detecting and quantifying levels of dopamine receptors expressed on the cell surface. In the biotinylation method, cell surface receptors are labeled with Sulfo-NHS-biotin. The charge on the sulfonyl facilitates water solubility of the reactive biotin compound and prevents its diffusion across the plasma membrane. In the ELISA method, surface labeling is achieved with antibodies specific to extracellular epitopes on the receptors, and by fixing the cells without detergent such that the plasma membrane remains intact.

Original languageEnglish (US)
Title of host publicationDopamine
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages3-13
Number of pages11
ISBN (Print)9781627032506
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume964
ISSN (Print)1064-3745

Fingerprint

Dopamine Receptors
Cell Surface Receptors
Biotinylation
Cell Membrane
Enzymes
Attention Deficit Disorder with Hyperactivity
Biotin
G-Protein-Coupled Receptors
Detergents
Solubility
Substance-Related Disorders
Cues
Epitopes
Schizophrenia
Water
Antibodies
Proteins

Keywords

  • ADHD
  • Biotinylation
  • DAPI
  • ELISA
  • Plasma membrane
  • Schizophrenia

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Xiao, J., & Bergson, C. M. (2013). Detection of cell surface dopamine receptors. In Dopamine: Methods and Protocols (pp. 3-13). (Methods in Molecular Biology; Vol. 964). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-251-3_1

Detection of cell surface dopamine receptors. / Xiao, Jiping; Bergson, Clare M.

Dopamine: Methods and Protocols. Humana Press Inc., 2013. p. 3-13 (Methods in Molecular Biology; Vol. 964).

Research output: Chapter in Book/Report/Conference proceedingChapter

Xiao, J & Bergson, CM 2013, Detection of cell surface dopamine receptors. in Dopamine: Methods and Protocols. Methods in Molecular Biology, vol. 964, Humana Press Inc., pp. 3-13. https://doi.org/10.1007/978-1-62703-251-3_1
Xiao J, Bergson CM. Detection of cell surface dopamine receptors. In Dopamine: Methods and Protocols. Humana Press Inc. 2013. p. 3-13. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-251-3_1
Xiao, Jiping ; Bergson, Clare M. / Detection of cell surface dopamine receptors. Dopamine: Methods and Protocols. Humana Press Inc., 2013. pp. 3-13 (Methods in Molecular Biology).
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